Cell-lysing strain, sludge reduction microbial inoculant and application thereof
A technology of sludge reduction and use of bacterial agents, which is applied in the direction of biological sludge treatment, bacteria, and separation of microorganisms, can solve the problems of harsh reaction conditions, difficulty in large-scale application, and high cost, and achieve easy control of reaction conditions and good Environmental benefit, low energy consumption effect
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Embodiment 1
[0045] Example 1: Isolation, screening, identification and application of dominant lytic bacteria
[0046] This embodiment mainly includes the following parts: 1. Separating and screening the dominant bacterial strains from domesticated sludge; 2. Identifying the dominant bacterial strains; determining the species relationship of the dominant bacterial strains; The screened dominant strains are the best process for sludge reduction treatment.
[0047] 1. Isolate and screen the dominant strains from the domesticated sludge, specifically including the following steps:
[0048] Step 1. Sludge domestication: domesticate the sewage sludge under the aeration condition of 0.1-1.0vvm, and at a temperature of 55-80°C, domestication time: 5-60 days;
[0049] Step 2, after carrying out the gradient dilution of the domesticated mature sludge, adopt the dilution pouring method and the three-section line method to separate and purify, obtain the bacterial strain of single colony, and the b...
Embodiment 2
[0071] Embodiment 2: Analysis of extracellular enzyme activity of dominant bacterial strain
[0072] Surplus sludge is a kind of organic wastewater containing a high concentration of suspended solids. Its main components include some small molecular soluble organic matter (monosaccharides, amino acids, etc.) and refractory macromolecular organic matter (mainly including proteins, polysaccharides, lipids compounds), among which carbohydrates accounted for 20% of the total organic matter. The study found that in the dissolving process of excess sludge, glucosidase, protease and α-amylase are closely related to the degradation of extracellular polymers in sludge, and the hydrolysis of protein is a key factor. Therefore, the activity of extracellular proteases secreted by bacteria is crucial.
[0073]Inoculate the dominant strains of the present invention Geobacillus sp. THE-14, Geobacillus sp. THE-4 and Geobacillus sp. THE-5 to the corresponding The protease activity was determ...
Embodiment 3
[0074] Example 3: Dissolving sludge performance of dominant strains
[0075] The dominant bacterial strains of the present invention are Geobacillus sp. THE-14, Geobacillus sp. THE-4, Geobacillus sp. THE-5, THE-14, THE-4 and THE -5 The mixed strains of the three and the control strain YL-1 were respectively inserted into the corresponding solid medium, activated and cultivated at 50-85°C for 12 hours, and the activated Geobacillus sp. ) THE-14, Geobacillus sp. THE-4 and Geobacillus sp. THE-5, and the three mixed strains were inoculated into a 250ml Erlenmeyer flask containing 50ml liquid medium, 50- Cultivate at 85°C for 12 hours, shake at a rate of 120rpm, and activate repeatedly for 3 times. After the activated mature cells were centrifuged in a desktop centrifuge (6000rpm, 10min), the cells were collected, and the cells were blown evenly with sterile water. The volume of dissolved sludge in each group was 360ml, and the volume of the added bacterial solution was 40ml. An ...
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