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High-potassium-solubilizing paenibacillus mucilaginosus strain and application thereof

A technology of Paenibacillus and Bacillus, which is applied to high-potassium-dissolving Paenibacillus colloidus strains and its application fields, can solve problems such as poor potassium-solubilizing ability, weak spore-producing ability, and inability to grow plants, so as to improve soil fertility and improve Organic matter and available potassium content, resistance-enhancing effect

Active Publication Date: 2021-10-26
微生物肥料技术研究推广中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Soil is a natural potassium pool, but it mainly exists in potassium-containing primary minerals (mica, potassium feldspar), which cannot be absorbed and utilized by plants
Paenibacillus colloidis can convert insoluble ineffective potassium in potassium-containing minerals such as potassium feldspar into soluble available potassium. Thus limiting its application as potash fertilizer

Method used

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  • High-potassium-solubilizing paenibacillus mucilaginosus strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 strain screening

[0017] The obtaining method of Paenibacillus colloidus GT018-JZ18112, it comprises the following steps:

[0018] 1) Prepare the cells of the type strain

[0019] (11) Isolate and screen the starting strain from wheat rhizosphere soil in Baoding, Hebei;

[0020] (12) The starting strain was subjected to high-temperature treatment at 60°C, 80°C, and 100°C for 20 minutes, then inserted into the potassium-dissolving medium, and cultivated in a constant temperature incubator at 32°C for 48 hours before use.

[0021] 2) Screening of strains with high potassium decomposing performance induced by ultraviolet rays

[0022] (21) Put the bacterial strain prepared in step 1 into 10ml sterile water, shake fully, and disperse the cells for later use;

[0023] (22) The solid potassium solution medium is poured into a plate, and it is set aside after solidification. The composition of the solid potassium solution medium is: 5-10% sucrose, Na 2 HPO 4 1-...

Embodiment 2

[0036] Embodiment 2 field application test

[0037] Cucumber field application test of Paenibacillus coliformis:

[0038] Test field selection: The test field is a fluvo-aquic soil with medium soil texture and medium fertility. The previous crop is tomato. The soil analysis was carried out on the plow layer before the test. The organic matter is 13.6g / kg, the total nitrogen is 1.18g / kg, and the available phosphorus is 11.5mg / kg. kg, available potassium 71mg / kg, pH value is 8.1, and the test crop is cucumber Zhongnong No. 106;

[0039] Test plan and method: This test set 4 treatments, 4 repetitions, using a completely randomized block design. Residential area 6.00m×5.00m=30.00m 2 .

[0040] Treatment 1: Regular fertilization.

[0041] Treatment 2: 10% reduction in conventional fertilization + 10 kg of Paenibacillus colloidus JZ18112 microbial inoculum.

[0042] Preparation of Paenibacillus colloidus GT018-JZ18112 microbial agent: Paenibacillus colloidis powder and diatom s...

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Abstract

The invention discloses a high-potassium-solubilizing paenibacillus mucilaginosus strain. The high-potassium-solubilizing paenibacillus mucilaginosus strain is paenibacillus mucilaginosus GT018-JZ18112 and is preserved in the China General Microbiological Culture Collection Center on May 7, 2019, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.17702. The high-potassium-solubilizing paenibacillus mucilaginosus strain has excellent potassium-dissolving capacity and can improve the soil fertility.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, and more specifically relates to a strain of Paenibacillus colloidus with high potassium dissolving capacity and its application. Background technique [0002] Potassium is one of the three elements of plant nutrition, which plays a very important role in the growth and development of plants, the formation of yield and the quality of plants. Tissues and organs with vigorous plant metabolic activities contain more potassium, and more than 60 kinds of enzyme activities in plants all involve potassium. Potassium can affect the metabolism of nitrogen and carbohydrates, and affect the synthesis of protein, starch and oil. Potassium plays an important role in the transport of substances in plants, and the transporting tissues (phloem cells) of plants contain more potassium (2%). Potassium-deficient plants have thinning mechanical tissues and cell walls, weak stems, easy to lodging...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C05G3/60C05G3/80C12R1/01
CPCC05F11/08C05G3/60C05G3/80
Inventor 江洋吴元凤张少骅孟庆伟马婷
Owner 微生物肥料技术研究推广中心
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