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Peripheral blood-based esophageal squamous carcinoma screening marker and kit

A technology of esophageal squamous cell carcinoma and markers, which is applied in the field of medicine and biology, can solve problems such as complex experimental steps, low sensitivity, and narrow detection dynamic range, and achieve good differentiation effect, high sensitivity and specificity, and good diagnostic effect

Pending Publication Date: 2021-10-29
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

NMR is characterized by no damage to the components to be measured, simple sample pretreatment, but low sensitivity and narrow detection dynamic range; GC-MS has good sensitivity and reproducibility, but derivatization methods are generally used for sample pretreatment , making the experimental steps more complicated

Method used

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  • Peripheral blood-based esophageal squamous carcinoma screening marker and kit
  • Peripheral blood-based esophageal squamous carcinoma screening marker and kit
  • Peripheral blood-based esophageal squamous carcinoma screening marker and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Screening of serum differential metabolic markers for esophageal squamous cell carcinoma

[0039] 1. Experimental samples

[0040] According to strict screening and exclusion criteria, 100 age- and sex-matched healthy subjects (normal control group) and 100 patients with esophageal squamous cell carcinoma (esophageal squamous cell carcinoma group) were collected from the First Affiliated Hospital of Zhengzhou University. The average age of 100 healthy subjects: 58.5 years old, ranging from 42-80 years old; the average age of 100 patients with esophageal squamous cell carcinoma: 59.5 years old, ranging from 42-83 years old.

[0041] The inclusion criteria for healthy subjects were: aged between 42 and 80 years old; no tumor was diagnosed by physical examination, no previous history of cancer; no other major systemic diseases; no chronic disease history of long-term medication.

[0042] The inclusion criteria for patients with esophageal squamous cell carcinom...

Embodiment 2

[0070] Example 2: Evaluation of the ability of differential metabolite diagnosis to distinguish patients with esophageal squamous cell carcinoma from healthy people

[0071] 1. The ability of a single differential metabolite diagnosis to distinguish patients with esophageal squamous cell carcinoma from normal people:

[0072] S-sulfo-L-cysteine ​​in the esophageal squamous cell carcinoma group (100 patients with esophageal squamous cell carcinoma) and normal control group (100 healthy subjects) serum samples detected based on UPLC-Q / TOF-MS in Example 1 acid, symmetric dimethylarginine, and 4-methoxyphenylacetic acid content data were analyzed, and the receiver operating curve (ROC curve) was used to evaluate each differential metabolite to diagnose and distinguish patients with esophageal squamous cell carcinoma and normal human ability. The ROC curves of S-sulfo-L-cysteine, symmetrical dimethylarginine, and 4-methoxyphenylacetic acid in differentiating patients with esophage...

Embodiment 3

[0096] Example 3: Application of three differential metabolites in the screening of esophageal squamous cell carcinoma

[0097] 1. Collection of serum samples

[0098] According to strict screening and exclusion criteria, 50 age- and gender-matched healthy subjects (normal control group) and 50 patients with esophageal squamous cell carcinoma (esophageal squamous cell carcinoma group) were collected from the First Affiliated Hospital of Zhengzhou University.

[0099] The inclusion criteria for healthy subjects were: aged between 42 and 80 years old; no tumor was diagnosed by physical examination, no previous history of cancer; no other major systemic diseases; no chronic disease history of long-term medication.

[0100] The inclusion criteria for patients with esophageal squamous cell carcinoma were: aged between 42 and 83 years old; patients with esophageal squamous cell carcinoma confirmed by endoscopy and histopathology, who had not received radiotherapy or chemotherapy; no...

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Abstract

The invention belongs to the technical field of medical biology, and particularly discloses a marker and a kit for diagnosing esophageal squamous carcinoma. The marker for diagnosing the esophageal squamous carcinoma, provided by the invention, is at least one of S-sulfo-L cysteine, symmetric dimethylarginine and 4-methoxyphenylacetic acid, and a detection reagent of the marker can be used for preparing a product for diagnosing the esophageal squamous carcinoma. The invention also provides a kit for diagnosing esophageal squamous carcinoma, the kit contains a detection reagent for detecting the marker, and the detection reagent is a reagent for detecting the marker in a sample through chromatography-mass spectrometry or chromatography-mass spectrometry. By detecting the expression levels of S-sulfo-L cysteine, symmetric dimethylarginine and 4-methoxyphenylacetic acid in human serum, esophageal squamous cell carcinoma can be effectively detected, the detection sensitivity reaches up to 80%, the specificity reaches up to 71%, and the kit can be used for large-scale screening of asymptomatic crowds in high-incidence areas of esophageal squamous cell carcinoma, and screening and early detection of asymptomatic high-risk groups are facilitated.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and specifically discloses a screening marker and kit for esophageal squamous cell carcinoma based on peripheral blood. Background technique [0002] Esophageal squamous cell carcinoma is one of the most common digestive system malignancies worldwide. According to the latest data from the World Health Organization (WH0) in 2018, there are 570,000 new cases of esophageal squamous cell carcinoma each year worldwide, and about 510,000 deaths, ranking seventh in cancer incidence and sixth in mortality worldwide. According to the analysis of the incidence and death of malignant tumors in China in 2015 released by the National Cancer Center, the number of new cases of esophageal squamous cell carcinoma in my country accounts for half of the world. [0003] Since the onset of esophageal squamous cell carcinoma is very insidious, early detection and diagnosis are extremely difficult. Clinic...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N27/62
CPCG01N30/02G01N27/62
Inventor 王立东徐帅杨苗苗胡景峰钟侃徐瑞华魏梦霞韩文莉雷玲玲赵学科宋昕王盼盼范宗民
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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