Method for extracting camellia polyphenol

An extraction method, the technology of camellia, is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, anti-toxins, etc., to achieve the effects of increasing the extraction rate, accelerating the dissolution rate, and reducing the extraction cost

Active Publication Date: 2021-11-02
广州果润化妆品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method uses more organic solvents

Method used

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  • Method for extracting camellia polyphenol
  • Method for extracting camellia polyphenol

Examples

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Embodiment 1

[0027] The extraction method of embodiment 1 camellia polyphenols

[0028] (1) Preparation of camellia powder: freeze and pulverize camellia for 10 minutes at -5°C, crush the frozen camellia with a cooking machine, and pass through a 14-mesh sieve.

[0029] (2) Preparation of extraction solution: Add camellia powder into the extraction solution, the weight ratio of solid to liquid is 1:5, the ultrasonic extraction time is 30min, the ultrasonic frequency is 10kHz, the ultrasonic power is 20W, and the ultrasonic temperature is 40°C. Extract solution, the biological enzyme is prepared by cellulase, isolated enzyme and superoxide dismutase with a mass ratio of 1:2:0.5, and the extract solution is composed of 20% acetone, 0.5% Biological enzyme, 2% glacial acetic acid with a volume fraction of 40% and the balance of water are prepared.

[0030] (3) Filtration: the adsorbent is added to the extract obtained in step (2), the mass ratio of the adsorbent and the extract is 0.05:1, the...

Embodiment 2

[0033] The extraction method of embodiment 2 camellia polyphenols

[0034] (1) Preparation of camellia powder: the camellia was frozen and pulverized for 15 minutes at a freezing temperature of 2° C., and the frozen camellia was pulverized with a cooking machine and passed through a 14-mesh sieve.

[0035] (2) Preparation of extraction solution: Add camellia powder into the extraction solution, the weight ratio of solid to liquid is 1:10, the ultrasonic extraction time is 40min, the ultrasonic frequency is 20kHz, the ultrasonic power is 25W, and the ultrasonic temperature is 50°C. Extract, the biological enzyme is made from cellulase, isolated enzyme and superoxide dismutase with a mass ratio of 3:5:1.5, and in mass percentage, the extract is composed of 40% acetone, 1.5% Biological enzyme, 5% volume fraction is 50% glacial acetic acid and the balance is made from water.

[0036] (3) Filtration: the adsorbent is added to the extract obtained in step (2), the mass ratio of the...

Embodiment 3

[0039] The extraction method of embodiment 3 camellia polyphenols

[0040] (1) Preparation of camellia powder: Frozen and pulverized camellias for 13 minutes at a freezing temperature of -1°C, pulverized the frozen camellias with a cooking machine, and passed through a 14-mesh sieve.

[0041] (2) Preparation of extraction solution: Add camellia powder into the extraction solution, the weight ratio of solid to liquid is 1:8, the ultrasonic extraction time is 35min, the ultrasonic frequency is 15kHz, the ultrasonic power is 23W, and the ultrasonic temperature is 45°C. Extract solution, the biological enzyme is prepared by cellulase, isolated enzyme and superoxide dismutase with a mass ratio of 2:3:1, and the extract solution is composed of 30% acetone, 1% Biological enzyme, 3% volume fraction is 45% glacial acetic acid and the balance is made from water.

[0042] (3) Filtration: the adsorbent is added to the extract obtained in step (2), the mass ratio of the adsorbent and the ...

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Abstract

The invention provides a method for extracting camellia polyphenol. The method comprises the following extraction steps: (1), preparing camellia powder: freezing, crushing and sieving camellia; (2), performing preparation of a leaching solution: adding camellia powder into the extracting solution, performing ultrasonic extraction, preparing the extracting solution, and preparing the extracting solution from acetone, bio-enzyme, glacial acetic acid and water; (3), performing filtering: adding an adsorbent into the extract liquid prepared in the step (2), performing oscillating, adsorbing and filtering to obtain polyphenol filtrate; (4), performing extraction: extracting the polyphenol filtrate obtained in the step (3) by using saturated n-butyl alcohol and ethyl acetate respectively to prepare a camellia polyphenol crude extract; and (5), performing concentrating and drying: concentrating and drying the camellia polyphenol crude extract prepared in the step (4) to obtain the camellia polyphenol. According to the extraction method, less organic solvent is used, the operation is simple, the extraction rate of the camellia polyphenol is higher than 19%, and the purity of the camellia polyphenol is higher than 95%.

Description

technical field [0001] The invention relates to the field of polyphenol extraction, in particular to a method for extracting camellia polyphenols. Background technique [0002] Polyphenols are one of the important components of natural medicines with various functional activities, which may have a good effect on the prevention of diseases. For example, polyphenols can prevent chronic diseases such as cardiovascular and cerebrovascular diseases, have a strong ability to scavenge free radicals, reduce oxidation, reduce the chance of tumor formation and reduce the risk of cancer. For example, CN201710612581.8 preparation method of tea polyphenols in Southwest Camellia, the method uses dried Southwest Camellia as raw material, reflux extraction with 50-80% acetone aqueous solution as solvent, the extract is concentrated and dried, then dissolved in water, filtered , after the filtrate is adsorbed by DM130 macroporous resin, the impurities are eluted with distilled water, and th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/82A61P39/06A61P9/00A61P35/00A61K133/00
CPCA61K36/82A61P39/06A61P9/00A61P35/00A61K2236/19A61K2236/333A61K2236/51
Inventor 李艳李梓源章文浩孙学华范冠宝
Owner 广州果润化妆品有限公司
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