Unlock instant, AI-driven research and patent intelligence for your innovation.

Sphingosine polycyclic aromatic hydrocarbon degrading strain JT-M9-H

A JT-M9-H, polycyclic aromatic hydrocarbon technology, applied in the field of pollutant treatment, can solve problems such as differences in treatment effects of treatment methods, and achieve the effects of good tolerance, efficient degradation and good adaptability

Active Publication Date: 2021-11-02
HENAN NORMAL UNIV
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, for organic pollutants in soil, commonly used chemical treatment methods, physical adsorption methods, etc., but due to the different types of organic pollutants, the applicable treatment methods are different, and at the same time, the treatment effects of different treatment methods are also different. Therefore, from the perspective of environmental safety, it is an "eternal" technical subject to explore suitable pollutant degradation treatment methods

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sphingosine polycyclic aromatic hydrocarbon degrading strain JT-M9-H
  • Sphingosine polycyclic aromatic hydrocarbon degrading strain JT-M9-H
  • Sphingosine polycyclic aromatic hydrocarbon degrading strain JT-M9-H

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment, screening can Sphingomonas sp provided herein JT-M9-H strain, the identification process of the present embodiment are described as follows.

[0030] (A) sample source

[0031] Consider PAHs degrading strain resistance required, the inventors Xinxiang Industrial Park Gu Tunsheng living sewage treatment plants as activated sludge sample source, both in order to obtain a filter having a certain tolerance, while the strain of microorganism capable of degrading polycyclic aromatic hydrocarbons .

[0032] (B) screening process

[0033] When screening strain, the screening process is substantially as follows: firstly sludge enriched microbial strain, followed by separation of the strain, then the combined degradation requires targeted screening. Refer to the following specific operations.

[0034] (A) Enrichment culturing:

[0035] 10 g of wet weight of bacteria source (i.e., industrial parks activated sludge samples) was added to the Erlenmeyer flask, add 90 mL steri...

Embodiment 2

[0046] JT-M9-H for Sphingomonas bacteria strains screened Example 1 determined, the potential for degradation of phenanthrene pyrene and human tolerance, cadmium invention has been explored for further study at different angles and clear experiment, the specific circumstances outlined below.

[0047] (A) at different concentrations Phoebe strains, growth at different tolerated cadmium concentration

[0048] Phoebe condition setting different concentrations:

[0049] Separate high concentrations of phenanthrene and phenanthrene pyrene various concentrations (dissolved in n-hexane, n-hexane until volatile), M9 medium and added with sterilized bacteria source, 30 ℃, 160 rpm shaking culture, nutrients will not be added during the culture, at regular intervals, 600nm absorbance measured at.

[0050] The specific growth curve figure 2 Indicated. As can be seen, with increasing concentration of phenanthrene (20 ~ 900mg / L), the growth JT-M9-H is gradually increased, when the Fe concentr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of pollutant treatment, and particularly relates to a sphingosine polycyclic aromatic hydrocarbon degrading strain JT-M9-H and an application of the sphingosine polycyclic aromatic hydrocarbon degrading strain JT-M9-H. The bacterial strain is named as sphingobium JT-M9-H, Sphingobium sp.JT-M9-H, and the preservation number of the bacterial strain is CCTCC (China Center for Type Culture Collection) NO: M2021248; and the strain is preserved in China Center for Type Culture Collection in Wuhan University, Wuhan, China on March 18, 2021. The sphingosine JT-M9-H strain provided by the invention not only can efficiently degrade polycyclic aromatic hydrocarbon pollutants, but also has relatively good tolerance to heavy metal cadmium ion pollution, relatively good adaptability to an oligophosphorus nutrition environment and relatively wide application range to pH, temperature and salinity of growth environment conditions. The sphingosine polycyclic aromatic hydrocarbon degrading strain JT-M9-H has good application potential for degradation treatment of related complex component pollutants.

Description

Technical field [0001] The present application belongs to the technical field of treatment of pollutants, in particular, to a degradation of polycyclic aromatic hydrocarbons in the genus Sphingomonas strain JT-M9-H patent application and its application matters. Background technique [0002] Soil as a contaminant of "sink", by the workers and peasants, mining and other human activities, different pollutants through atmospheric deposition, irrigation and industrial sewage leaks and other ways simultaneously or successively into the same soil, after exceeding its self-cleaning ability, the formation of a combined pollution of soil types. A typical composite contaminants such as POPs hydrophobic polycyclic aromatic hydrocarbons (PAHs) and inorganic contaminants cadmium (Cd), widely present in the soil near the sewage tank, and coking smelters, power plants and coal chemicals middle. Since the composite contaminants in the soil have synergistic or antagonistic interactions, universal...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02B09C1/10A62D101/20C12R1/01
CPCA62D3/02B09C1/10A62D2101/20Y02W10/10
Inventor 李静华欧懿文郑悦陈婷常义爽袁凤玲袁铭玥陈明牛苗苗张昌甜
Owner HENAN NORMAL UNIV