Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Pinctada fucata pallium cell in-vitro culture solution as well as preparation method and use method thereof

A technology of pearl oyster and mantle cells is applied in the field of preparation, in vitro culture medium of mantle cells of n. The effect of improving the survival rate

Pending Publication Date: 2021-11-02
三亚热带水产研究院 +1
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the in vitro culture of mantle cells, the cells often enter the decay stage prematurely, resulting in the inability to complete subsequent operations
[0004] So far, there has been no report on the use of Chinese herbal ingredients to improve the survival rate of cells in vitro

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Basal cell culture medium 200ml / L, tea polyphenols 1.5ml / L, fetal bovine serum 3.5ml / L, pearl oyster body fluid 2.5ml / L, kanamycin 0.05g / L, balanced salt solution 200ml / L, glucose 100ml / L L, add double distilled water to make up to 1L. After being sterilized by ultraviolet lamps, all the mixtures are evenly mixed, and can be used for in vitro culture of Hepu pinnacle mantle cells under the condition of ensuring sterility and constant temperature.

Embodiment 2

[0021] Basal cell culture medium 300ml / L, tea polyphenols 1.7ml / L, fetal bovine serum 2.5ml / L, pearl oyster body fluid 2.0ml / L, kanamycin 0.05g / L, balanced salt solution 200ml / L, glucose 100ml / L L, add double distilled water to make up to 1L. After being sterilized by ultraviolet lamps, all the mixtures are evenly mixed, and can be used for in vitro culture of Hepu pinnacle mantle cells under the condition of ensuring sterility and constant temperature.

Embodiment 3

[0023] Basal cell culture medium 250ml / L, tea polyphenols 2.0ml / L, fetal bovine serum 2.5ml / L, pearl oyster body fluid 3.0ml / L, kanamycin 0.08g / L, balanced salt solution 200ml / L, glucose 100ml / L L, add double distilled water to make up to 1L. After being sterilized by ultraviolet lamps, all the mixtures are evenly mixed, and can be used for in vitro culture of Hepu pinnacle mantle cells under the condition of ensuring sterility and constant temperature.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of cell culture, and discloses a pinctada fucata pallium cell in-vitro culture solution, a preparation method and a use method, and the cell in-vitro culture solution comprises the following components: a basic cell culture medium, tea polyphenol, fetal calf serum, pearl oyster body fluid, kanamycin, a balanced salt solution and glucose. The Chinese herbal medicine extract tea polyphenol, pearl oyster body fluid and other components are added into the basic cell culture medium, the purposes of bacteriostasis, oxidation resistance and cell aging resistance are achieved in a reasonable proportion, and the pearl oyster body fluid culture medium has the advantages of being free of toxic and side effects, not prone to causing environmental pollution and free of residues; and the survival rate of mantle cell culture is increased, the operation cost is reduced, the operation difficulty is reduced, and the bottleneck of mantle cell in-vitro culture is broken through.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to an in vitro culture medium, a preparation method and a use method of the mantle cells of Hepu pinnacle oyster. Background technique [0002] Pinctada fucata, also known as Pinctada martensii, belongs to Mollusca, Lamellibranchia, Pterioida, and Pearl Oyster in terms of animal taxonomy. (Pteriidate) and Pinctada are the main shellfish species that produce seawater pearls in the world, and are also one of the main shellfish that produce seawater cultured pearls "South Pearl" in my country. Hepu pearl oysters are mainly distributed in tropical and subtropical waters. In my country, they are mainly distributed in the coastal areas of Hainan, Guangdong, Guangxi, Taiwan and other provinces. In foreign countries, they are distributed in coastal countries on the west coast of the Pacific Ocean, from the south of Chiba County in Japan to the Philippines, Vietnam, It is dis...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/07
CPCC12N5/0601C12N2500/76C12N2500/82
Inventor 方伟陈明强李有宁王雨邓正华魏海军于刚
Owner 三亚热带水产研究院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com