Heparin binding protein determination kit, preparation method and use method

Abstract

The invention discloses a heparin binding protein determination kit, which is characterized by comprising a solid-phase marker, a tracing marker, a neutralization marker, a cleaning solution and a substrate; the solid-phase marker comprises a coated antibody and a solid-phase support, and the coated antibody is marked on the solid-phase support; the tracing marker comprises a labeled antibody and a tracer, and the labeled antibody is labeled on the tracer; the neutralization marker comprises one of microspheres or magnetic beads and a labeled antibody labeled on the microspheres or the magnetic beads; the labeled antibody in the neutralization marker and the labeled antibody in the tracing marker are antibodies aiming at the same epitope; the cleaning solution comprises a buffer solution and a surfactant, and the substrate is a substrate corresponding to the tracer. The kit is combined with a chemiluminescence immunoassay analyzer to detect the content of the heparin binding protein in a sample, and the kit is high in detection sensitivity, good in reproducibility, high in accuracy and wide in detection linear range.

Description

technical field [0001] The invention belongs to the field of in vitro diagnostic reagents, in particular to a heparin-binding protein assay kit, a preparation method and a use method. Background technique [0002] Heparin-binding protein (HBP), also known as azurocidin or CAP37, is a granule protein derived from neutrophils. HBP is a multifunctional and active homologous serine protease with a relative molecular mass of 37kDa. The structure of HBP is a single-chain protein containing 222 amino acids, containing 8 cysteine ​​residues, with a glycosylation site on the 100th, 114th or 145th aspartic acid residue, and the structure is similar to neutral Granulocyte elastase has 45% homology with it and 30%-37% homology with other granule-derived serine proteases. Approximately 74% of HBP is stored in azurophilic granules, 18% in secretory vesicles, and 8% on the plasma membrane. [0003] Studies have shown that HBP can be used as an early diagnostic marker for infectious dise...

AI Technical Summary

Problems solved by technology

However, the amount of protein coated on the ELISA microwell plate of this kit is limited, which in turn affects the content of the immune sandwich complex formed after the reaction, resulting in a narrow linear range of the kit, and the disadvantages of cumbersome operation and long detection time

[0010] CN204882574U discloses a test kit for detecting heparin-binding protein by colloidal gold method, and CN204882575U discloses a test kit for detecting heparin-binding protein by immunofluorescence chromatography. Although these two methods can be detected quickly, they can only be qualitatively or semi-quantitatively detection, the accuracy is low, and the results with high accuracy cannot be provided

[0011] Both CN11929443A and CN112255416A reported a chemiluminescent detection reagent, but both belong to conventional detection principles, and are easily affected by the excess of antigens, resulting in the detection linear range of the kit is not wide enough to meet the clinical needs

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