Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method of bimetallic nano-enzyme composite material with in-situ tumor microenvironment regulation and anti-tumor effects

A bimetallic nanotechnology, tumor microenvironment technology, applied in non-active ingredients medical preparations, medical preparations containing active ingredients, antitumor drugs, etc., can solve the problem of low tumor microenvironment response sensitivity and nanozyme catalytic efficiency It can improve the efficiency of tumor treatment, optimize the enzymatic reaction conditions, and achieve the effect of small nano-particle size.

Active Publication Date: 2021-11-12
HARBIN ENG UNIV
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problems of low nanoenzyme catalytic efficiency and low response sensitivity of tumor microenvironment in the existing tumor treatment, and provide a bimetallic nanoenzyme composite material with in situ regulation of tumor microenvironment and anti-tumor effect preparation method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of bimetallic nano-enzyme composite material with in-situ tumor microenvironment regulation and anti-tumor effects
  • Preparation method of bimetallic nano-enzyme composite material with in-situ tumor microenvironment regulation and anti-tumor effects
  • Preparation method of bimetallic nano-enzyme composite material with in-situ tumor microenvironment regulation and anti-tumor effects

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0047] Specific Embodiment 1: In this embodiment, a method for preparing a bimetallic nanoenzyme composite material with in situ regulation of tumor microenvironment and anti-tumor effect is characterized in that the preparation method is specifically completed according to the following steps:

[0048] 1. Synthesis of bimetallic nanoparticles CoFe 2 o 4 :

[0049] ①. Add the cobalt source and the iron source to the solvent respectively, and stir evenly to obtain a mixed solution A;

[0050] ②. Put the mixed solution A in a three-necked round bottom flask, then vacuumize, and then raise the temperature of the mixed solution A to 90°C~120°C under the condition of magnetic stirring, and then magnetically stir and the temperature is 90°C~120°C. Reaction, obtain mixed solution B;

[0051] ③. Introduce nitrogen into the mixed solution B, then raise the temperature of the mixed solution B to 260°C-298°C, then react at 260°C-298°C, cool to room temperature, and obtain the reaction...

specific Embodiment approach 2

[0067] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the cobalt source described in step one 1. is cobalt acetylacetonate; the iron source described in step one 1. is iron acetylacetonate; The solvent mentioned is dibenzyl ether, tetraethylene glycol or octadecene. Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0068] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the mass ratio of cobalt source and iron source described in step one 1. is 1: (0.5~2); The mass ratio of the total mass of the cobalt source and the iron source to the solvent is 1:(50-80). Other steps are the same as those in Embodiment 1 or 2.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
The average diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides a preparation method of a bimetallic nano-enzyme composite material with in-situ tumor microenvironment regulation and anti-tumor effects, relates to a preparation method of a nano-enzyme composite material, and aims to solve the problems of low nano-enzyme catalytic efficiency and low tumor microenvironment response sensitivity in existing tumor treatment. The method comprises the following steps: 1, synthesizing bimetallic nanoparticles CoFe2O4; 2, preparing a bimetallic nano-enzyme composite material; 3, performing surface modification to obtain the bimetallic nano-enzyme composite material with in-situ tumor microenvironment regulation and anti-tumor effects. According to the method, the tumor microenvironment is regulated in situ by using GOD enzyme activity and multi-element enzyme activity of nano-enzyme, the enzymatic reaction conditions are optimized, the endogenous power of the tumor microenvironment is utilized to the maximum extent, and the tumor treatment efficiency is increased. By adopting the method, the bimetallic nano-enzyme composite material with in-situ tumor microenvironment regulation and anti-tumor effects can be obtained.

Description

technical field [0001] The invention relates to a preparation method of a nano-enzyme composite material. Background technique [0002] Traditional tumor treatment methods, such as surgery, chemotherapy, and radiotherapy, have poor selectivity, large side effects, and high drug resistance caused by continuous drug use. The development of nanotechnology provides new ideas for solving the above problems. The finely tunable structure and morphology of nanomaterials and the modifiable surface properties directly determine their interaction with biomolecules, cells, tissues, organs and individuals, and thus produce unique biological effects—nanobiological effects. In-depth study of nano-biological effects from individual, cellular and molecular levels, and elucidation of its precise mechanism, has become a very challenging and hot frontier. In 2004, Pasquato et al. named nanomaterials with enzyme catalytic activity as nanozymes for the first time. Nanozyme, as a new type of ar...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K33/26A61K33/24A61K47/69A61P35/00B82Y5/00B82Y30/00B82Y40/00C01G51/00
CPCA61K33/26A61K33/24B82Y30/00B82Y5/00B82Y40/00A61K47/6923A61P35/00C01G51/00C01P2004/64A61K2300/00
Inventor 丁鹤杨飘萍贺飞盖世丽杨丹刘志亮冯莉莉常金虎
Owner HARBIN ENG UNIV