Rigidity-controllable three-dimensional porous gel scaffold material as well as preparation method and application thereof

Abstract

The invention discloses a rigidity-controllable three-dimensional porous gel scaffold material and a preparation method and application thereof. According to the scaffold material, calcium carbonate / glucolactone is adopted as a composite ionic cross-linking system to prepare alginate hydrogel, then silk fibroin / collagen is compounded and encapsulated in the gel, so pores and pore diameters in the hydrogel are reduced, porous structures which are communicated with one another are increased, the adhesion force of the silk fibroin / collagen on the hydrogel is increased, and the porous structures are more beneficial to adhesion and growth of cells. The three-dimensional tissue engineering scaffold material provided by the invention has good biocompatibility, is beneficial to adhesion of cells and acceleration of a tissue regeneration process, is beneficial to differentiation of bone mesenchymal stem cells, and further promotes formation of new bones. The preparation method disclosed by the invention is simple, relatively low in cost and easy to operate and control, and has a very wide application prospect in the field of bone tissue engineering.

Description

technical field [0001] The invention relates to the technical field of tissue engineering materials, in particular to a three-dimensional porous gel scaffold material with controllable stiffness and its preparation method and application. Background technique [0002] Tissue engineering is an emerging discipline that combines cell biology and material science to construct tissues or organs in vitro or in vivo. Reconstruction in three aspects, structure and function, and achieve permanent replacement. Regenerative tissue engineering refers to planting isolated autologous high-concentration stem cells on some kind of cell scaffold or extracellular matrix after in vitro culture and expansion, and then implanting this cell hybrid material into the tissue defect site, and gradually degrading the biological material At the same time, the planted stem cells continue to differentiate and proliferate, so as to achieve the purpose of repairing tissue defects. In order to obtain well...

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Problems solved by technology

[0005] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a three-dimensional porous gel scaffold material with controllable stiffness and its preparation method and application, so as to solve the problem that the existing two-dimensional scaffold materials lack the ability of cells to activate and differentiate in vivo. The real three-dimensional matrix mechanical microenvironment in which the behavior is performed, and the quasi-three-dimensional platform cannot accurately simulate the stiffness of the bone matrix, etc.

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