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Preparation method of double-membrane-coated bionic nano diagnosis and treatment probe

A bionic nanometer and membrane-coated technology, which is applied in the research field of tumors and related diseases, can solve the problems of poor biocompatibility, poor targeting, and poor targeting, and achieve strong immune escape ability, prolong blood circulation time, and high The effect of tumor targeting ability

Pending Publication Date: 2021-11-19
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the problems of poor biocompatibility and targeting of the existing nano-delivery systems still need to be solved urgently. In the application, it is found that the nano-delivery system is not only easy to be recognized and cleared by the immune system, but also most of the existing delivery systems have poor targeting, etc. shortcoming

Method used

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  • Preparation method of double-membrane-coated bionic nano diagnosis and treatment probe
  • Preparation method of double-membrane-coated bionic nano diagnosis and treatment probe
  • Preparation method of double-membrane-coated bionic nano diagnosis and treatment probe

Examples

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Embodiment 1

[0038] A preparation method of a double-membrane-coated biomimetic nano-diagnosis probe, comprising the steps of:

[0039] (1) Preparation of long-lasting nanomaterials PLNPs by means of solvothermal method;

[0040] Preparation of long afterglow nanomaterials PLNPs: Accurately weigh 1.4874g of Zn(NO 3 ) 2 ·6H 2 O solid was put into a 50mL round bottom flask, and 10mL Ga(NO 3 ) 3 solution (0.6M), stirring, until Zn(NO 3 ) 2 After fully dissolved, add 300 μL Cr(NO 3 ) 3 solution (0.1M), 1500μL Yb(NO 3 ) 3 solution (0.1M), 150μL Er(NO 3 ) 3 Solution (0.1M) and 10mL ammonium germanate solution (0.1M) were mixed and stirred, and the pH was adjusted, and a coprecipitation reaction occurred to obtain a reaction solution. , pH 8.0) after 24 hours of reaction, the product was washed by centrifugation and roughly ground with an agate mortar, then calcined at 1000°C for 1 hour to obtain the long-lasting nano-luminescent material PLNPs;

[0041] Size screening of long afterg...

Embodiment 2

[0047] A preparation method of a double-membrane-coated biomimetic nano-diagnosis probe, the steps and methods are basically the same as those in Example 1, except that the target cells are tumor cells and platelets.

Embodiment 3

[0049] A preparation method of a double-membrane-coated biomimetic nano-diagnosis probe, the steps and methods are basically the same as in Example 1, except that the target cells are tumor cells and Lactobacillus reuteri. The extraction process of the tumor cell membrane is as follows. The tumor cells in the logarithmic growth phase are digested and collected by centrifugation, and then dispersed in Tris buffer (pH7.4) containing 0.1% protease inhibitors. Crush in an ice bath for 10 minutes, centrifuge at 3200g for 5 minutes, then take the supernatant and centrifuge at 20000g for 30 minutes, and then take the supernatant and centrifuge at 80000g for 90 minutes. The obtained pale yellow solid is the tumor cell membrane. Tumor cells were stored in a -20°C refrigerator for future use. The extraction process of the Lactobacillus reuteri biofilm is as follows. Select the target bacteria for cultivation. When the OD600 value of the bacteria is 0.5 with a microplate reader, centrifu...

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Abstract

The invention relates to a preparation method of a double-membrane-coated bionic nano diagnosis and treatment probe. The preparation method comprises the following steps: (a) preparing a long-afterglow nano material PLNPs by virtue of a solvothermal method; (b) modifying mesoporous silica on the surface of the long-afterglow nano material by a tetraethoxysilane hydrolysis method to obtain PLNPs@SiO2; and (c) coating the surface of the PLNPs@SiO2 with double biological membranes through a liposome extrusion method to obtain the PLNPs@SiO2@CMM. The bionic nano diagnosis and treatment probe has the beneficial effects that the bionic nano diagnosis and treatment probe taking long afterglow as a luminous core is successfully prepared, and the mesoporous silica and the dual-biological hybrid membrane are modified on the surfaces of the long afterglow nano particles, so that a safe and effective synergistic treatment effect can be generated on tumors and related diseases.

Description

technical field [0001] The invention belongs to the research field of tumors and related diseases, and relates to a preparation method of a long afterglow bionic nanometer probe for diagnosis and treatment. Background technique [0002] In recent years, the morbidity and mortality of malignant tumors have been increasing year by year, which not only seriously endangers human health, but also creates a heavy burden for society. Early diagnosis and treatment of tumors is conducive to improving the efficacy of tumor treatment and prolonging the survival period of patients. Therefore, how to diagnose and track tumors through bioimaging is very important for the precise treatment of tumors. Fluorescence bioimaging technology, which has been flourishing recently, can collect the fluorescence emitted by living organisms through sensitive camera equipment filters. With the assistance of imaging contrast agents, fluorescence bioimaging technology can directly observe the complete ph...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K47/02A61K47/46A61K47/52A61K47/69A61P35/00C12N5/09C12N5/0786C12N1/20C12N1/06C12R1/225
CPCA61K49/0019A61K49/0056A61K49/005A61K47/02A61K47/46A61K47/52A61K47/6949A61P35/00B82Y40/00B82Y5/00B82Y20/00B82Y30/00C12N5/0693C12N5/0645C12N1/20C12N1/066
Inventor 王硕刘敬民王志豪
Owner NANKAI UNIV
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