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Method for preparing structural grease by adopting enzyme method

A technology of enzymatic preparation and structured lipids, which is applied in the field of deep processing of oils and fats, can solve the problems of unsuitability for industrial production, many by-products, and difficult reactions, etc., and achieve the effects of low raw material cost, avoiding difficult control, and high yield

Pending Publication Date: 2021-11-23
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The hydrolysis method is a method of generating diglycerides by enzymatically catalyzing the hydrolysis of triglycerides. The products of the hydrolysis method depend on the degree of reaction. The products of partial hydrolysis are monoglycerides, diglycerides and free fatty acids, and the products of complete hydrolysis are Free fatty acids and glycerin, the hydrolysis reaction is not easy to control, and there are many by-products, so it is not suitable for industrial production

Method used

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  • Method for preparing structural grease by adopting enzyme method
  • Method for preparing structural grease by adopting enzyme method
  • Method for preparing structural grease by adopting enzyme method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) Accurately weigh 20.0g of soybean oil sample, 20.0g of distilled water, and 0.047g of lipase AY "Amano" 400SD derived from Candidacylindracea and add them to the beaker, seal it, control the reaction temperature to 35°C, and react under magnetic stirring 4h. After the reaction, lipase AY "Amano" 400SD and distilled water are recovered to obtain a fat mixture rich in free fatty acids. As detected by HPLC-ELSD, the content of free fatty acids is shown in Table 1.

[0047] (2) Take 24.0g of oil mixture, add 1.0g of glycerin, and add 1.21g of lipase Lipozyme RM IM derived from Rhizomucor miehei to catalyze the reaction, control the reaction temperature at 65°C, and react for 8h under magnetic stirring to control the temperature of the reaction system The pressure is 100Pa. After the reaction, the lipase Lipozyme RM IM is recovered. The product is washed with water, centrifuged to remove glycerin, and molecularly distilled at 0.5Pa and 180°C to obtain a product rich in ...

Embodiment 2

[0049] Same as Example 1, but the mass ratio of soybean oil and distilled water in step (1) is modified to 10:1 (distilled water 2.0g), and all the other parameters remain unchanged.

[0050] Detected by HPLC-ELSD, the content of free fatty acid in step (1) and the yield of diglyceride in step (2) are shown in Table 1.

Embodiment 3

[0052] Same as Example 1, but the mass ratio of soybean oil and distilled water in step (1) is modified to 20:1 (distilled water 1.0g), and all other parameters remain unchanged.

[0053] Detected by HPLC-ELSD, the content of free fatty acid in step (1) and the yield of diglyceride in step (2) are shown in Table 1.

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Abstract

The invention discloses a method for preparing structural grease by adopting an enzyme method, and belongs to the field of deep processing of grease. The method comprises the following steps: 1), carrying out hydrolysis reaction on a lipase catalysis oil-water system with hydrolytic activity on glyceride, and recovering lipase and water after the reaction is finished, so as to obtain a grease mixture rich in free fatty acid; and 2) adding glycerol, adopting lipase with esterification activity to catalyze glycerol and free fatty acid to generate esterification reaction to synthesize diglyceride. Compared with a chemical method, the method is mild in reaction condition and environment-friendly. Compared with a method for preparing diacylglycerol through direct esterification of glycerol and fatty acid, the method disclosed by the invention is low in raw material cost and high in diacylglycerol yield, and meanwhile, the defects that the reaction for preparing diacylglycerol through a hydrolysis method is difficult to control and many by-products are generated are avoided. The method adopted by the invention is low in production cost, the product is easy to separate, and meanwhile, diglyceride with higher yield is prepared.

Description

technical field [0001] The invention relates to a method for preparing structured lipid by enzymatic method, which belongs to the field of deep processing of oil. Background technique [0002] Diacylglycerol (DAG) is a structural lipid in which one fatty acid in a triglyceride is substituted by a hydroxyl group, and has special physiological functions. Diacylglycerol can be divided into two stereoisomers, and its molecular structure is shown in Formula 1, wherein R or R1 is a fatty acid group: [0003] [0004] Animal and human experiments have shown that 1,3-DAG is digested by the human body to generate glycerol and free fatty acids, which are converted into energy in the human body. Compared with triglyceride, the unique metabolic characteristics of 1,3-DAG make it in It plays a certain role in controlling hyperlipidemia and obesity. In addition, as an important type of polyol-type nonionic surfactant, glyceryl glycolate has lubricating, antistatic, emulsifying and ot...

Claims

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Application Information

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IPC IPC(8): C12P7/64
CPCC12P7/6454
Inventor 王小三江聪陈烨时旭旺黄雅祺王熠璠王笑寒
Owner JIANGNAN UNIV