Indicator capable of quickly responding to tiny pH value change as well as preparation method and application of indicator
An acid-base indicator and fast-response technology, applied in the field of biochemistry, can solve the problems of ineffective indication of discoloration range, narrow mutation range, and detection limit, etc., and achieve rapid response to pH changes, obvious discoloration, and rapid detection
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Embodiment 1
[0025] Example 1 The preparation of an ultra-sensitive indicator with a mutation color point of pH 6.2
[0026] Prepare 0.015% lactoferrin solution: weigh 1.5mg lactoferrin and dissolve it in 10mL glycerin; prepare 0.2% nitrazine indicator: weigh 0.2g nitrazine and dissolve it in 100mL absolute ethanol ; Preparation of 0.1mol / L citric acid solution: take 21.01g of citric acid and dissolve it in 1000mL of pure water; Mix 19.0mL citric acid solution and 81.0mL sodium citrate solution to make a pH6.0 SSC buffer solution; mix the above-mentioned 100μL lactoferrin solution and 1.5mL nitrate solution evenly, and use the pH6.0 SSC solution to make it volume to 10mL to make an ultra-sensitive indicator with a mutation color point of pH 6.2.
[0027] Such as figure 1 As shown, soak the filter paper or dust-free cloth with the ultra-sensitive indicator with a mutation point of pH 6.2 and dry it, then add detection solutions with different pH values dropwise. When the pH ≥ 6.2, the i...
Embodiment 2
[0028] Example 2 Preparation of an ultra-sensitive indicator whose mutation color point is pH 4.4
[0029] Prepare 0.025% myosin solution: weigh 2.5mg of myosin to dissolve and dilute to 100mL glycerin; prepare 0.1% bromocresol green-nitrazine indicator: weigh 0.02g bromocresol green and 0.08g Nitrazine reagent, dissolved and fixed volume in 100mL absolute ethanol; prepare 0.1mol / L citric acid solution: take 21.01g of citric acid and dissolve it in 1000mL pure water, prepare 0.1mol / L sodium citrate solution: take Sodium citrate 29.41g was dissolved and fixed in 1000mL pure water; 65.5mL citric acid solution and 34.5mL sodium citrate solution were mixed to make a SSC buffer solution with pH 4.0; the above 50μL myosin solution and 2mL bromocresol The green-nitrazine indicator was mixed evenly, and the volume was adjusted to 10 mL with pH 4.0 SSC solution to prepare an ultra-sensitive indicator with a mutation color point of pH 4.4.
Embodiment 3
[0030] Example 3 Preparation of Ultra-sensitive Indicator with Mutant Chromogenic Point of pH 3.8
[0031] Prepare 0.02% bovine serum albumin solution: weigh 20mg lactoferrin and dissolve it in 100mL glycerol; prepare 0.47% bromocresol green-methyl orange-methyl red indicator: weigh 0.25g bromocresol at the same time Green, 0.1g methyl orange, 0.12g methyl red reagent, dissolve and dilute in 100mL absolute ethanol; prepare 0.1mol / L sodium acetate solution: take 8.2g sodium acetate and dissolve and dilute in 1000mL pure water; prepare 0.1mol / L acetic acid solution: 2.86mL acetic acid solution was diluted to 500mL with ultrapure water; 15.0mL acetic acid solution and 75.0mL sodium acetate solution were mixed to make an ABS buffer solution with pH 3.5; the above 200μL bovine serum albumin solution and Mix 1.5mL of bromocresol green-methyl orange-methyl red indicator evenly, and dilute to 10mL with pH 3.5 ABS solution to make an ultra-sensitive indicator with a mutation color poin...
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