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Method for increasing polysaccharide content of shiitake mushroom variety 808

A technology for polysaccharide content and variety is applied in the field of increasing the polysaccharide content of the Lentinus edodes variety 808, and achieves the effects of increasing the polysaccharide content of the Lentinus edodes, having a good application prospect and enhancing the additional economic benefits of the Lentinus edodes.

Active Publication Date: 2021-11-30
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Based on the above analysis, a method to regulate the polysaccharide content of Lentinus edodes mycelia by selecting specific metal ions and appropriate concentrations has not yet been found in the industry

Method used

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  • Method for increasing polysaccharide content of shiitake mushroom variety 808
  • Method for increasing polysaccharide content of shiitake mushroom variety 808
  • Method for increasing polysaccharide content of shiitake mushroom variety 808

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of Lentinus edodes 808 Fermentation Broth

[0033] 1.1 Preparation and identification of mother species

[0034] Choose 808, the main variety of shiitake mushrooms, inoculate it on a PDA slant medium plate (200g of potatoes, 20g of glucose, 20g of agar), place it at 25°C, and activate the strains under dark conditions.

[0035] 1.2 Preparation of fermentation medium

[0036] Based on the results of 1.1, after the 10d mycelia covered the plate, start to prepare the fermentation medium (g / L): (sucrose 35g, peptone 5g, yeast powder 2.5g, KH 2 PO 4 1g, MgSO 4 0.5g, vitamin B1 0.5g, natural pH), using a 250ml Erlenmeyer flask with a capacity of 50ml, sterilizing in an autoclave at 121°C for 30min. After the culture medium is cooled, use a hole puncher to punch holes on the activated plate to make strain blocks, and inoculate 5 strain blocks in each bottle of fermentation medium.

Embodiment 2

[0038] Biomass of Lentinus edodes mycelia under different metal ions and their concentrations

[0039] After the Lentinus edodes bacterial strain of Example 1 and the culture medium were prepared, 5 kinds of metal ions (Na + 、K + , Ca 2+ , Fe 2+ and Mn 2+ ), static culture at 25°C. Biomass and total polysaccharide content of different metal ions and their concentrations at 60d as figure 1 and figure 2 shown.

[0040] The amount of biomass indicates the size of mycelium growth rate, from figure 1 It can be seen that different metals can promote the growth of mycelium, but there are differences in the optimal induction concentration of different metals (P2+ When the concentration is 200mg / L, the biomass is the highest, indicating that Fe at this concentration 2+ The promotion effect on mycelial growth is the most obvious, much higher than the induction of other metal ions, which is about 2 times that of no metal ions. From figure 2 As a result, it can be found that o...

Embodiment 3

[0042] Different Fe 2+ Polysaccharide content of Lentinus edodes mycelium at induction time

[0043] Experimental condition is the same as embodiment 2, and the polysaccharide content of shiitake mushroom mycelia is as follows: image 3 shown. according to image 3 From the results we can see that when adding Fe 2+ After treatment, the content of intracellular polysaccharide (IPS) was higher than that of the control, and the content added after 7 days of shaker culture was the highest, that is, the Fe(2) group. The content of intracellular polysaccharide reached 2.61%, which was 2.51 times that of the control. The exopolysaccharide (EPS) was higher than that of the control group, that is, the Fe(3) group, which was 0.32 mg / mL, which was 1.21 times that of the control group, only when it was added to the shaker culture and then rested for 10 days. The total polysaccharides increased significantly after 10 days of rest in the shaker culture, that is, the Fe(3) group reached ...

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Abstract

The invention discloses a method of a culture medium formula for increasing content of lentinan. The method comprises the following specific steps: preparing a culture medium, preparing a mother strain, preparing fermentation liquor, adding Fe<2+> with a proper concentration, inoculating, standing and culturing at 25 DEG C for 60 d, and collecting mycelia to extract polysaccharide; and preparing a cultivation bag, adding Fe<2+> with a proper concentration, inoculating, mycelium growing, fruiting, collecting sporocarps, and extracting sporocarp polysaccharide. Exogenous metal ions with a proper concentration are added within optimal induction time, growth of mycelia is promoted, expression quantity and enzyme activity of a key enzyme gene for polysaccharide synthesis are improved, the polysaccharide content of the shiitake mushroom mycelia is obviously improved, meanwhile, Fe <2+> with a proper concentration improves activity of a key enzyme for sporocarp polysaccharide synthesis, and the sporocarp polysaccharide content is increased. The method provides support for optimizing a shiitake mushroom fermentation mode, improving the content of shiitake mushroom mycelia and sporocarp polysaccharide and boosting development of shiitake mushroom processing industry.

Description

technical field [0001] The invention belongs to the technical field of agricultural planting, and in particular relates to a method for increasing the polysaccharide content of shiitake mushroom variety 808. Background technique [0002] Mushrooms (Lentinus edodes) belong to Basidiomycetes, Agaricaceae, Pleurotaceae, also known as mushrooms, winter wild rice, flower mushrooms and so on. Our country has a long history of shiitake cultivation and covers a wide range. Among them, the shiitake mushroom variety 808 has the best benefits and the best quality. It is currently the main shiitake mushroom variety in my country. Because shiitake mushroom has a special fragrance, it is very popular among consumers. Its production and consumption in the world are second only to Agaricus bisporus. It is also one of the important industries with strong competitiveness in the adjustment of my country's agricultural industry structure. Lentinan is one of the main active ingredients in Lenti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/38C12P19/04A01G18/40A01G18/20C12R1/645
CPCC12N1/14C12N1/38C12P19/04A01G18/40A01G18/20
Inventor 向泉桔侯诗瑶何茂兰张慧娟辜运富陈强
Owner SICHUAN AGRI UNIV
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