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Method for increasing yield of microalgae source fucoxanthin
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A technology of algae-derived rock and algae xanthin, which is applied in the field of increasing the yield of microalgae-derived fucoxanthin, can solve the problems of culture failure, no successful cases, and harsh culture conditions, so as to achieve easy industrial production, increase yield, The effect of simple method
Pending Publication Date: 2021-11-30
JINAN UNIVERSITY
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However, so far, except for the commercial production of fucoxanthin by cultivating Phaeodactylum tricornutum (Phaeodactylum tricornutum) by the Israeli company Algatechnologies, there are no other successful cases.
The main reason is that marine microalgae have strict requirements on temperature, light and other cultivation conditions, and the existing cultivation systems (outdoor track pool, pipeline photobioreactor, etc.) cannot effectively control the cultivation conditions, and are also prone to contamination of bacteria, fungi, Protozoa or miscellaneous algae, resulting in extremely low biomass concentration and fucoxanthin content obtained, or even culture failure, which in turn exacerbates the high production cost of fucoxanthin
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Embodiment 1
[0032] Example 1: Phase changes of cell growth and fucoxanthin accumulation in Odontella aurita cultured under LED red light, blue light and full-spectrum white light.
[0033] Experimental material: Odontella aurita (expanded and cultivated)
[0034] Preparation of medium: 1.5g / L NaNO 3 , 20.758g / L NaCl, 3.477g / L NaCl 2 SO 4 , 0.587g / L KCl, 0.17g / L NaHCO 4 , 9.395g / L MgCl 2 ·6H 2 O, 1.316g / L CaCl 2 2H 2 O, 0.0845g / L KBr, 0.0225g / L H 3 BO 3 , 0.0027g / L NaF, 0.0214g / L SrCl 2 ·6H 2 O, 0.00436g / L EDTA-Na 2 , 0.0315g / LFeCl 3 ·6H 2 O, 1.781×10 -4 g / L MnCl 2 4H 2 O, 2.3×10 -5 g / L ZnSO 4 4H 2 O, 1.19×10 -5 g / LCoCl 2 ·6H 2 O, 2.5×10 -6 g / L CuSO 4 ·5H 2 O, 1.84×10 -6 g / L Na 3 VO 4 , 1.94×10 -6 g / L KCrO 4 , add the above ingredients to 1L deionized water, after 121℃, 20min high temperature sterilization treatment, add 0.6g / L NaSiO which has been sterilized 3 4H 2 O, 0.03g / L NaH 2 PO 4 ·H 2 O, 2×10 -4 μg / L Vitamin B1, 1×10 -6 Biotin, 1 x 10 -6 μg / L v...
Embodiment 2
[0039] Example 2: Effects of Different LED Red Light and Blue Light Ratio on the Growth, Fucoxanthin Accumulation and Yield of Odontella aurita
[0040] Experimental material: Odontella aurita (expanded and cultivated)
[0041] Preparation of medium: 1.5g / L NaNO 3 , 20.758g / L NaCl, 3.477g / L NaCl 2 SO 4 , 0.587g / L KCl, 0.17g / L NaHCO 4 , 9.395g / L MgCl 2 ·6H 2 O, 1.316g / L CaCl 2 2H 2 O, 0.0845g / L KBr, 0.0225g / L H 3 BO 3 , 0.0027g / L NaF, 0.0214g / L SrCl 2 ·6H 2 O, 0.00436g / L EDTA-Na 2 , 0.0315g / LFeCl 3 ·6H 2 O, 1.781×10 -4 g / L MnCl 2 4H 2 O, 2.3×10 -5 g / L ZnSO 4 4H 2 O, 1.19×10 -5 g / LCoCl 2 ·6H 2 O, 2.5×10 -6 g / L CuSO 4 ·5H2 O, 1.84×10 -6 g / L Na 3 VO 4 , 1.94×10 -6 g / L KCrO 4 , add the above ingredients to 1L deionized water, after 121℃, 20min high temperature sterilization treatment, add 0.6g / L NaSiO which has been sterilized 3 4H 2 O, 0.03g / L NaH 2 PO 4 ·H 2 O, 2×10 -4 μg / L Vitamin B1, 1×10 -6 Biotin, 1 x 10 -6 μg / L Vitamin B12. .
[0042] e...
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Abstract
The invention provides a method for increasing the yield of microalgae source fucoxanthin. The method comprises the following steps of: 1) obtaining an odontoglossum longibrachiatum strain from an alga strain chamber, transferring the odontoglossum longibrachiatum into a culture bottle filled with an mL1 liquid culture medium, and performing static culture; 2) transferring the odontoglossum longibrachiatum strain cultured in the step 1) into a 1000mL culture bottle filled with the mL1 liquid culture medium, and performing static culture; 3) inoculating the odontoglossum longibrachiatum strain cultured in the step 2) into a closed photobioreactor; 4) washing the strain obtained in the step 3) with a fresh mL1 liquid culture medium, and transferring into a 20L closed photobioreactor according to the initial inoculation density OD750 of 0.60 + / -0.02; 5) adjusting the power of red light and blue light to be 1: 9, 2: 8, 3: 7, 4: 6, 5: 5, 6: 4, 7: 3, 8: 2 and 9: 1 respectively; and 6) measuring the biomass concentration at regular time every day in the culture process. A foundation is laid for developing a fair-price fucoxanthin product and meeting the healthy and high-quality life of people.
Description
technical field [0001] The invention relates to the field of microalgae biological resources and biotechnology, in particular to a method for increasing the yield of microalgae source fucoxanthin. Background technique [0002] Fucoxanthin is a natural carotenoid with multiple beneficial effects on human health, and its molecular formula is C 42 h 58 o 6 , which is characterized by the molecule containing 5,6-monoepoxide and ethylenic bond. As a light-harvesting pigment, fucoxanthin is widely found in the cells of most marine microalgae and macroalgae. Compared with macroalgae, marine microalgae not only have the advantages of high fucoxanthin content (up to 100 times the content in macroalgae), but also have the advantages of fast growth, high photosynthetic efficiency, carbon sequestration and emission reduction, and available The photobioreactor has the advantages of year-round production and is an ideal raw material for the production of fucoxanthin. However, so far,...
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