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Production method of two-channel microfluidic cathode photoelectrochemical sensor based on SOD@Au@PANI signal amplification

A photoelectrochemical and signal amplification technology, applied in chemical instruments and methods, electrochemical variables of materials, material analysis through optical means, etc., can solve the problems of cumbersome detection methods, high detection limit, high cost, etc., and achieve automatic The effect of detection, low detection limit, and strong ability to resist the interference of reducing substances

Inactive Publication Date: 2021-11-30
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are many detection methods for cardiac troponin, such as radioimmunoassay, enzyme-linked immunoassay, electrochemiluminescence analysis, etc., but most of the detection methods are cumbersome, complicated to operate, expensive, and have high detection limits. Therefore, It is of great significance to establish a fast, simple and sensitive detection method

Method used

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  • Production method of two-channel microfluidic cathode photoelectrochemical sensor based on SOD@Au@PANI signal amplification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A preparation method of a dual-channel microfluidic cathode photoelectrochemical sensor based on SOD@Au@PANI signal amplification, the preparation steps are as follows:

[0028] (1) Use the computer design software AUTOCAD to design and draw the graphics of the dual-channel microfluidic chip;

[0029] (2) Use a 3D printer to print the designed channel pattern, and make a dual-channel microfluidic chip with polydimethylsiloxane PDMS. The dual-channel microfluidic cathode photoelectrochemical sensor is made of a microfluidic screen-printed electrode base plate, and The fluidic lower chip and the microfluidic upper chip are composed of three parts. Among them, the bottom plate of the microfluidic screen printing electrode is indium tin oxide ITO conductive glass, which is used as an etching double working electrode, printed conductive carbon paste as a counter electrode, and printed Ag- AgCI slurry is used as a reference electrode, the microfluidic lower chip includes work...

Embodiment 2

[0038] A preparation method of a dual-channel microfluidic cathode photoelectrochemical sensor based on SOD@Au@PANI signal amplification, the preparation steps are as follows:

[0039] (1) Use the computer design software AUTOCAD to design and draw the graphics of the dual-channel microfluidic chip;

[0040] (2) Use a 3D printer to print the designed channel pattern, and make a dual-channel microfluidic chip with polydimethylsiloxane PDMS. The dual-channel microfluidic cathode photoelectrochemical sensor is made of a microfluidic screen-printed electrode base plate, and The fluidic lower chip and the microfluidic upper chip are composed of three parts. Among them, the bottom plate of the microfluidic screen printing electrode is indium tin oxide ITO conductive glass, which is used as an etching double working electrode, printed conductive carbon paste as a counter electrode, and printed Ag- AgCI slurry is used as a reference electrode, the microfluidic lower chip includes work...

Embodiment 3

[0049] A preparation method of a dual-channel microfluidic cathode photoelectrochemical sensor based on SOD@Au@PANI signal amplification, the preparation steps are as follows:

[0050] (1) Use the computer design software AUTOCAD to design and draw the graphics of the dual-channel microfluidic chip;

[0051] (2) Use a 3D printer to print the designed channel pattern, and make a dual-channel microfluidic chip with polydimethylsiloxane PDMS. The dual-channel microfluidic cathode photoelectrochemical sensor is made of a microfluidic screen-printed electrode base plate, and The fluidic lower chip and the microfluidic upper chip are composed of three parts. Among them, the bottom plate of the microfluidic screen printing electrode is indium tin oxide ITO conductive glass, which is used as an etching double working electrode, printed conductive carbon paste as a counter electrode, and printed Ag- AgCI slurry is used as a reference electrode, the microfluidic lower chip includes work...

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Abstract

The invention relates to a production method of a two-channel microfluidic cathode photoelectrochemical sensor based on SOD@Au@PANI signal amplification. The two-channel microfluidic cathode photoelectrochemical sensor is composed of a micro-fluidic screen printing electrode bottom plate, a micro-fluidic lower chip and a micro-fluidic upper chip, the micro-fluidic screen printing electrode bottom plate is indium tin oxide (ITO) conductive glass and is used as an etching double working electrode, a printing conductive carbon paste is used as a counter electrode, a printing Ag-AgCI paste is used as a reference electrode, and a Pd / I-BiOBr-OVs nano material is used for modifying a working electrode to obtain a relatively strong cathode light current signal; and the three electrodes of the cathode photoelectrochemical two-channel are integrated on the microfluidic cathode photoelectrochemical sensor, automatic detection can be realized under the control of an injection pump, and an accurate detection result can be quickly obtained without human interference. The two-channel microfluidic cathode photoelectrochemical sensor can realize rapid, efficient, sensitive and automatic detection of cardiac troponin cTnI.

Description

technical field [0001] The invention relates to a dual-channel microfluidic cathode photoelectrochemical sensor, specifically, a method for preparing a dual-channel microfluidic cathode photoelectrochemical sensor based on SOD@Au@PANI signal amplification is designed. Background technique [0002] Acute myocardial infarction is a very serious disease. With the improvement of people's living standards, the incidence of myocardial infarction is becoming more and more widespread, including middle-aged and elderly people, family history of coronary heart disease, hypertension, diabetes, dyslipidemia, smoking, Long-term staying up late and high workplace stress are all high-risk groups for acute myocardial infarction, and the incidence rate is increasing year by year, but the age of onset is decreasing year by year. Clinical attention should be paid attention to. Early detection and early treatment can greatly increase the cure rate; therefore, early diagnosis has important clini...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/17G01N27/26B01L3/00
CPCG01N21/17G01N27/26B01L3/502707B01L2300/12
Inventor 杜宇徐坤魏琴冯金慧马洪敏任祥
Owner UNIV OF JINAN