Biosensor based on silver@polydopamine nanosphere composite structure and preparation method

A technology of dopamine nanometer and biosensor, which is applied in material analysis using radiation diffraction, material analysis using radiation, Raman scattering, etc. It can solve problems such as low detection limit, low protein sensitivity, and poor biocompatibility. Achieve the effect of simple and green preparation process, enhanced Raman signal, and low cost

Pending Publication Date: 2021-12-03
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many deficiencies in the biosensors on the market, such as low sensitivity of biosensors to specific proteins, low detection limit, poor specificity, poor stability, poor biocompatibility, etc.

Method used

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  • Biosensor based on silver@polydopamine nanosphere composite structure and preparation method
  • Biosensor based on silver@polydopamine nanosphere composite structure and preparation method
  • Biosensor based on silver@polydopamine nanosphere composite structure and preparation method

Examples

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Effect test

preparation example Construction

[0043] A method for preparing a biosensor based on a silver@polydopamine nanosphere composite structure, comprising the following steps:

[0044] S1, synthesizing polydopamine;

[0045] S2, adding the purified polydopamine aqueous solution into a round-bottomed flask, slowly adding ice-cold silver nitrate aqueous solution dropwise under magnetic stirring, and reacting;

[0046] S3. Take the prepared silver@polydopamine aqueous solution into a centrifuge tube, add 4-MBA ethanol solution, vortex and store;

[0047] S4. Add 100 μL of 500 μM SH-PEG-COOH to step S3, and continue to store;

[0048]S5. Add EDC / NHS (EDC 40mg / ml; NHS 110mg / ml), and shake gently in the dark, then add cTn I-Antibody to react;

[0049] S6, adding BSA solution, reacting and sealing, that is, the prepared nanolabel;

[0050] S7. Add the nano-label and the serum to the centrifuge tube successively, vortex, place it at room temperature, and drop it onto the grooved glass slide for reaction.

[0051] Furth...

Embodiment 1

[0061] Preparation of three-dimensional silver@polydopamine nanocomposite material, the specific steps are as follows:

[0062] (1) Dissolve 0.5 g of dopamine hydrochloride in 10 mL of ultrapure water and set aside. Add 2mL of ammonia water, 40mL of absolute ethanol, and 90mL of ultrapure water into a round-bottomed flask, and stir with an open magnetic force for 30min at room temperature. Then quickly add the dopamine aqueous solution, open the reaction at room temperature for 24 to 48 hours, and centrifugally purify the polydopamine aqueous solution and ethanol at a ratio of 1:3, and finally disperse in the aqueous solution.

[0063] (2) Add 5 mL of the purified polydopamine aqueous solution into a round-bottomed flask, slowly add 8 mL of 10 mM ice-cold silver nitrate aqueous solution dropwise under magnetic stirring, and react for 1 h.

[0064] The specific steps for preparing a biosensor from the above-mentioned three-dimensional silver@polydopamine nanocomposite material...

Embodiment 2

[0074] The specific steps of preparing the three-dimensional silver@polydopamine nanocomposite material are the same as those in Example 1.

[0075] The specific steps for preparing a biosensor from the above-mentioned three-dimensional silver@polydopamine nanocomposite material are as follows:

[0076] (3) with embodiment 1.

[0077] (4) with embodiment 1.

[0078] (5) After 24 hours, at room temperature of 25°C, add EDC / NHS (EDC 40mg / ml; NHS 110mg / mL 5μL each), and after 15min in the dark, add 10μL 0.1mg / mL CK-MB- Antibody, react at 4°C for 24h.

[0079] (6) With embodiment 1.

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Abstract

The invention discloses a biosensor based on a silver@polydopamine nanosphere composite structure and a preparation method. The composite structure is characterized in that silver nanoparticles with the diameter of 5-30nm are uniformly loaded on polydopamine nanospheres with the diameter of about 350-450nm, Raman molecularly 4-mercaptobenzoic acid is adsorbed on the surfaces of the polydopamine nanospheres, the polydopamine has excellent biocompatibility and oxidation resistance, so that rapid oxidation of the silver nanoparticles and inactivation of protein can be prevented, and by utilizing the excellent surface enhanced Raman effect of the silver nanoparticles, the marker detection can be realized only by acquiring related data through a laser Raman spectrometer. The invention also discloses an application of the sensor in Raman detection of a myocardial infarction biomarker-troponin. According to the present invention, the troponin detection sensitivity is high, the specificity is high, and the biocompatibility is high.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a biosensor based on a silver@polydopamine nanosphere composite structure and a preparation method. Background technique [0002] Acute myocardial infarction (AMI) is myocardial necrosis caused by acute and persistent coronary ischemia and hypoxia. Clinically, there are often severe and persistent substernal pain, which cannot be completely relieved by rest and nitrates, accompanied by increased serum myocardial enzyme activity and progressive ECG changes, which may be complicated by arrhythmia, shock or heart failure, which can be life-threatening in severe cases . Troponin (cTn I) is currently recognized as a reliable indicator for the diagnosis of myocardial infarction with the highest specificity and the longest duration. cTn I<0.2μg / L is normal; >1.5μg / L is the critical value for diagnosis (using ELISA method). The cTn I concentration in the blood gener...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N23/20G01N23/04
CPCG01N21/65G01N23/20G01N23/04
Inventor 王丁侯旭敏何斌刘韦卓鲍丽萍王现英李慧珺
Owner UNIV OF SHANGHAI FOR SCI & TECH
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