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Alpha2 macroglobulin rapid detection kit and detection method

A technology for detection kits and macroglobulins, which is applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of long detection time, inaccuracy, and imprecise detection methods, and achieve the effect of reducing protein decomposition and deformation

Active Publication Date: 2021-12-03
长春晨裕伽康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problems of impreciseness, inaccuracy and long detection time of the above existing detection methods, the present invention provides a new α2 macroglobulin rapid detection kit and detection method, the scheme is as follows:

Method used

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  • Alpha2 macroglobulin rapid detection kit and detection method
  • Alpha2 macroglobulin rapid detection kit and detection method
  • Alpha2 macroglobulin rapid detection kit and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Preparation of reagent a

[0045] Reagent aⅠ

[0046] Reagent a: Includes phosphate buffered saline.

[0047] Reagent aⅡ

[0048]Reagent a: including phosphate buffer; also including lauryl ether, the concentration is 4.0g / L.

[0049] The preparation method of the reagent a includes: adding all the substances included in the reagent a into the buffer solution therein, and mixing them uniformly.

[0050] Reagent aⅢ

[0051] Reagent a: includes phosphate buffer; also includes lauryl ether, the concentration is 4.0g / L; also includes ethylene glycol, zinc sodium EDTA. The concentration of polyethylene glycol is 0.06wt%, and the concentration of zinc sodium EDTA is 0.07wt%

[0052] The preparation method of the reagent a includes: adding all the substances included in the reagent a into the buffer solution therein, and mixing them uniformly.

[0053] It has been verified that the formulation of the reagent a provided by the present invention can stabilize the...

Embodiment 2

[0063] Example 2 Preparation of reagent b

[0064] Reagent bⅠ

[0065] Reagent b: includes goat anti-human α2-macroglobulin antibody; also includes phosphate buffered saline, the concentration of goat anti-human α2-macroglobulin antibody is 100 mg / mL.

[0066] The preparation method of the reagent b includes: adding all the substances included in the reagent b into the buffer solution therein, and mixing them uniformly.

[0067] Compared with reagent bⅡ, the antibody was slightly turbid. It can be seen that the formula ratio at this time is not stable enough to use goat anti-human α2-macroglobulin antibody alone, and the anti-interference ability of the detection kit is weak.

[0068] Reagent bⅡ

[0069] Preparation of goat anti-human α2-macroglobulin antibody-coated latex particles: take polystyrene latex particles with a particle size of 120 nm and a surface carboxylation of 200 nm in a ratio of 3:1, add N-carbamoylmethyl ethanesulfonic acid with a pH of 6.5 Acid (ACES) b...

Embodiment 3

[0101] Embodiment 3 α2-macroglobulin detection method, comprises the following steps:

[0102] S1. Mix reagent a with the sample, mix, and incubate;

[0103] S2. Read the absorbance value and record it as absorbance value I;

[0104] S3. Add reagent b, mix, incubate, and read the absorbance value again, which is recorded as absorbance value II;

[0105] S4. Calculate the difference between the absorbance value II and the absorbance value I, and according to the difference between the concentration of α2 macroglobulin in the standard solution and the obtained difference, make a Logit-Logit5P function curve of the regression equation of the difference to the concentration of α2 macroglobulin in the sample , according to the difference obtained from the sample to be tested, and substitute it into the function curve to calculate the concentration of α2 macroglobulin in the sample to be tested.

[0106] The amount of sample described in S1 is 30.0 μl, the amount of reagent a is 200...

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Abstract

The invention discloses an alpha2 macroglobulin rapid detection kit and a detection method, and relates to the technical field of biological detection methods. The kit comprises the following components: a reagent a comprising a phosphate buffer solution; a reagent b comprising an anti-alpha2 macroglobulin antibody; a standard substance solution, namely a solution containing an alpha2 macroglobulin standard substance; and a quality control solution, namely a solution containing an alpha2 macroglobulin standard substance. When the kit is used for rapidly detecting the alpha2 macroglobulin, an obtained regression function curve has high linearity in a range of 15-500mg / L, the detection limit is 15mg / L, the detection time can be shortened to about 5min from about 15min of an existing method, and the detection speed is greatly improved.

Description

technical field [0001] The invention relates to the technical field of biological detection methods, in particular to an α2 macroglobulin rapid detection kit and a detection method. Background technique [0002] α2-macroglobulin (α2-macroglobulin, α2MG or AMG) is the protein with the largest molecular weight in plasma. The molecular weight is about 652,000-800,000, the sugar content is about 8%, and it is composed of 4 subunits. It is closely related to the development and function of lymphoreticular system cells. The most prominent property of α2MG is that it can bind to a variety of molecules and ions. In particular, it can combine with many proteolytic enzymes and affect the activity of these enzymes. Such as binding to many endopeptidases including serine, sulfhydryl, carboxyl proteolytic enzymes and some metalloproteolytic enzymes. These proteolytic enzymes include plasmin, pepsin, chymotrypsin, trypsin, and cathepsin D. Studies have shown that the interaction of α...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N33/96G01N21/31
CPCG01N33/68G01N33/577G01N33/96G01N21/31G01N2333/4713
Inventor 朱积宝王旭
Owner 长春晨裕伽康生物科技有限公司
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