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Method for constructing specific chromatograms of horsetail and horsetail preparation

A construction method and a technology of characteristic maps, which are applied in the field of construction of characteristic maps of equisetum and its preparations, can solve the problems of complicated preparation method of test solution, short preparation time of test solution, good separation of characteristic maps, etc. The effect of shortening preparation time, peak response value, good separation effect and large number of characteristic peaks

Active Publication Date: 2021-12-07
BEIJING KANGRENTANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007]Therefore, the technical problem to be solved in the present invention is to overcome the time-consuming measurement of horsetail and its preparation characteristic spectrum in the prior art, low efficiency, and the method for preparing the test solution. Complicated, can not take into account the shortcomings of short preparation time of the test solution and good resolution of the characteristic map, so as to provide a method for constructing the characteristic map of horsetail and its preparations

Method used

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  • Method for constructing specific chromatograms of horsetail and horsetail preparation
  • Method for constructing specific chromatograms of horsetail and horsetail preparation
  • Method for constructing specific chromatograms of horsetail and horsetail preparation

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preparation example Construction

[0061] The preparation method of horsetail standard decoction freeze-dried powder includes: taking horsetail decoction pieces, placing them in a casserole, soaking for 30 minutes, adding water 14 times the amount of the decoction pieces after decocting, boiling with high fire, decocting with slow fire for 20 minutes, filtering while hot, cooling, Set aside; add 10 times the amount of water to the decoction pieces for the second decoction, boil with strong fire, decoct with slow fire for 15 minutes, filter while it is hot, and cool for later use; combine the filtrates, concentrate under reduced pressure, and freeze-dry to obtain. The present invention has used 18 batches of horsetail standard decoction freeze-dried powder, and the batch number and the origin of each batch of horsetail standard decoction freeze-dried powder are as follows:

[0062]

[0063]

[0064] The preparation method of horsetail formula granules includes: taking horsetail decoction pieces, adding 16 t...

Embodiment 1

[0077] This embodiment provides a method for constructing a characteristic map of horsetail and its preparation, comprising the following steps,

[0078] (1) Preparation of the reference substance solution of the reference substance: take 4mg kaempferol, accurately weighed, add 200ml of methanol with a volume fraction of 75%, and make a 20 μg / ml kaempferol reference substance solution;

[0079] (2) Preparation of contrasting medicinal material reference substance solution: get 0.6g horsetail contrasting medicinal material, put in a stoppered Erlenmeyer flask, add 30ml of mixed solvent (75% methanol 24ml, hydrochloric acid 6ml) of volume fraction 75% methanol and hydrochloric acid aqueous solution, Weigh the weight, heat to reflux for 1 hour, take it out, let it cool, weigh again, make up the lost weight with a mixed solvent of methanol and hydrochloric acid with a volume fraction of 75%, shake well, filter the filtrate, and obtain the reference substance solution of the referen...

experiment example 1

[0092] The investigation of experimental example 1 wavelength

[0093]Get horsetail test sample (lot number is S1), prepare need testing solution according to the method in embodiment 1 step (3), then need testing solution is carried out 210-400nm full-wavelength scanning, obtains the three-dimensional chromatogram of full-wavelength scanning Figure, see Figure 4 , the results show that the peak at 310nm is more abundant, and the peak shape is better than other wavelengths. Therefore, 310nm is selected as the detection wavelength.

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Abstract

The invention belongs to the technical field of drug detection, and particularly relates to a construction method of specific chromatograms of horsetail and horsetail preparation. According to the method, a to-be-detected horsetail sample is determined by adopting high performance liquid chromatography, and the specific chromatogram of the horsetail is obtained. According to the method, a mixed solution of methanol and hydrochloric acid is used as a solvent for preparing a test solution, so that the preparation method of the test solution is greatly simplified, the operation is simple and convenient, the preparation time of the test solution is shortened, the optimal elution condition is established, and the detection duration of the specific chromatograms of the horsetail and the horsetail preparation is shortened; the characteristic peaks obtained by the construction method of the characteristic chromatograms of the horsetail and the horsetail preparation are relatively good in peak shape and separation degree, relatively good in repeatability, and relatively good in separation efficiency, peak capacity and sensitivity. The method is short in analysis time and high in working efficiency, and has the advantages of being simple, convenient, stable, high in precision, good in repeatability, scientific and reliable.

Description

technical field [0001] The invention belongs to the technical field of drug detection, and in particular relates to a method for constructing characteristic maps of horsetail and preparations thereof. Background technique [0002] Equisetum is one of the traditional Chinese medicines. It is the dried aboveground part of Equisetum hyemale L., a plant of the family Equisetum. , liver meridian. Traditional medicine believes that horsetail has the effect of evacuating wind-heat, improving eyesight and reducing cloudiness. It is used for wind-heat red eyes, wind-flowing tears, and cloudy eyes. [0003] At present, the drug forms of horsetail on the market include horsetail decoction pieces, standard horsetail decoction, horsetail formula granules, and some compound medicines containing horsetail. Equisetum standard decoction freeze-dried powder is obtained by decocting horsetail decoction pieces with water twice, filtering while hot, combining filtrates, concentrating under red...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/8634
Inventor 张志强付静史国华贾扬花王改丽
Owner BEIJING KANGRENTANG PHARMA
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