Preparation method of guar gum enzymatic hydrolysate with high GM ratio
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A technology for guar gum and enzymatic hydrolysis products is applied in the field of preparation of guar gum enzymatic hydrolysis products to achieve the effects of promoting intestinal development, improving intestinal antioxidant and immune capabilities, and improving growth performance and disease resistance.
Active Publication Date: 2021-12-10
NANJING YIXIAN BIOTECHNOLOGY CO LTD
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Problems solved by technology
At present, there are no reports on the directional preparation process and functional differences of guar gum enzymatic hydrolyzates of different GM ratios. Research on the preparation technology and functional effects of guar gum enzymatic hydrolyzates with specific structures is conducive to promoting guar gum enzymatic hydrolysis Targeted application of products in actual production
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Embodiment 1
[0039] Example 1: The present invention provides a high Gm ratio of Guar bean adhesively preparation method, such as Figure 9 As shown, the specific steps are as follows:
[0040] 1 kg Guoli adhesive in the autoclave of 135 ° C pressure degradation 2 h, in accordance with the mass ratio of solid fluid than 1:15, the high-pressure degradation of Guar bean gum is filled with hydrochloric acid, and the pH is 4.0 to each gram. The pressurized degradation substrate was added to the beta-mannan 1000 u, and the plant hydrolase 700 U was enzymatically hydrolyzed, and the temperature of the temperature of 55 ° C was reacted 6 h, after the reaction, the enzymatic hydrolyzide was cooled, horizontal The spiral centrifuge is separated from the supernatant, concentrated by vacuum reduction (the vacuum is -0.08 MPa, temperature 60 ° C, time 2 h), spray dry, and produce high Gm ratio of Guar beans enzymatic hydrolysis product.
[0041] The molecular weight distribution of the high Gm ratio of the...
Embodiment A
[0050] Preferred Example A, a special mating feed for promoting sequivial sequenisencinity and intestinal health development, including the following weight materials:
[0051] raw material Proportion Fish powder 8% Wheat flour 10% Glutinum 4% Enzymatic algae powder 36% Sea motor 38.5% Phospholipid 1% Polyvilty 1% Multi-ore 1% High GM ratio of Guar bean adhesive 0.1% Composite lysozyme 0.4%
[0052] Each raw material in the formulation is mixed in a scale in the table, 60 S is mixed in a mixer at a rotational speed of 200 R / min, and stirred at a mass ratio of 15 min according to the mass ratio of the raw material 2: 1, and a reunion wet material is prepared.
Embodiment B
[0053] Example B, a special mating feed for promoting sequivial sexy and intestinal health development, including the following weight materials:
[0054] raw material Proportion Fish powder 8% Wheat flour 10% Glutinum 4% Enzymatic algae powder 36% Sea motor 38% Phospholipid 1% Polyvilty 1% Multi-ore 1% High GM ratio of Guar bean adhesive 0.2% Composite lysozyme 0.8%
[0055] Each raw material in the formulation is mixed in a scale in the table, 60 S is mixed in a mixer at a rotational speed of 200 R / min, and stirred at a mass ratio of 15 min according to the mass ratio of the raw material 2: 1, and a reunion wet material is prepared.
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Abstract
The invention provides a preparation method of a guar gum enzymolysis product with a high GM ratio, which comprises application of the guar gum enzymolysis product in a young sea cucumber feed, guar gum solid powder is used as a raw material, and the guar gum enzymolysis product with the high GM ratio is directionally prepared through solid pressurization, composite enzymolysis, centrifugal concentration and spray drying. The method has the advantages that the high-pressure degradation technology and the composite enzymolysis technology are combined, the molecular weight of the product is reduced through high-pressure-assisted enzymolysis, the GM ratio of the product is directionally increased through composite enzymolysis, and the guar gum enzymolysis product with the high GM ratio is a novel functional guar gum enzymolysis product. The guar gum enzymolysis product with the high GM ratio, the compound lysozyme and various nutritional raw materials are reasonably compounded, so that the intestinal development of the young sea cucumber can be effectively promoted, the antioxidant and immunocompetence of the intestinal tract can be improved, the intestinal flora can be adjusted, the intestinal function of the young sea cucumber can be comprehensively strengthened, the burden of bait conversion on the intestinal function of the young sea cucumber during the culture period of the young sea cucumber can be solved, the growth performance and disease resistance of the young stichopus japonicus are improved, and the adult stichopus japonicus quality and culture benefits are guaranteed.
Description
Technical field [0001] The present invention relates to the field of biotechnology, and is specifically a method for preparing a high Gm ratio of Guar bean enzymatic product. Background technique [0002] Sea Cemetery is one of my country's traditional Haizhen. At present, domestic artificial breeding technology is relatively mature, but the farming industry is still facing many problems, especially in the period of the young cucumber, the intestinal environment is fragile, and the bait is quickly converted from algae to artificially mixing feed, more prone to appear. Issues where physique and disease resistance have declined, increasing the risk of young ginseng disease and death. The intestinal tract is a place for sea cucumber nutrition and absorption, and it is also the first immune defense line against pathogens intrusion, where a large amount of microorganisms affect the digestion, metabolism and regeneration of sea cucumber. The seedlings have not yet developed, the intest...
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