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ELISA kit for auxiliary diagnosis of esophageal squamous carcinoma

A technology for auxiliary diagnosis and esophageal squamous cell carcinoma, applied in the field of medicine and biology, to achieve the effect of good diagnosis effect and good differentiation effect

Active Publication Date: 2021-12-10
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • ELISA kit for auxiliary diagnosis of esophageal squamous carcinoma
  • ELISA kit for auxiliary diagnosis of esophageal squamous carcinoma
  • ELISA kit for auxiliary diagnosis of esophageal squamous carcinoma

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Example 1: Using human proteome chips to screen markers for the diagnosis of esophageal squamous cell carcinoma

[0041] 1. Experimental sample:

[0042] The sera of 30 patients with esophageal squamous cell carcinoma (esophageal squamous cell carcinoma group) and 24 normal subjects (normal control group) were collected from the specimen bank of the Henan Provincial Key Laboratory of Cancer Epidemiology; among them, the serum of 30 patients with esophageal squamous cell carcinoma Patients with esophageal squamous cell carcinoma confirmed by pathology without any treatment; 24 normal human serums were obtained from healthy subjects, and the inclusion criteria for healthy subjects were: no cardiovascular, respiratory, liver, kidney, gastrointestinal tract , endocrine, blood, mental, or nervous system diseases and the history of the above diseases, no acute or chronic diseases, no evidence of tumor-related, no history of drug allergy, and the results of clinical laboratory...

Embodiment 2

[0061] Example 2: ELISA detects the serum expression levels of autoantibodies against tumor-associated antigens MAGEA1, VCL, TP53, and PRKCZ, and evaluates the value of four anti-tumor-associated antigen autoantibodies for the diagnosis of esophageal squamous cell carcinoma

[0062] Enzyme linked immunosorbent assay (ELISA) was used to detect the expression levels of the four anti-tumor-associated antigen autoantibodies screened in Example 1 in human serum, and the four anti-tumor-associated antigen autoantibodies were evaluated for use in esophageal squamous cell carcinoma. value in cancer diagnosis.

[0063] 1. Experimental sample:

[0064] The samples of 90 patients with esophageal squamous cell carcinoma (esophageal squamous cell carcinoma group) and 90 normal controls (healthy control group) included in this study were all obtained from the specimen bank of the Key Laboratory of Cancer Epidemiology in Henan Province; among them, 90 cases of esophageal squamous cell carcin...

Embodiment 3

[0101] Example 3: ELISA was used to detect the serum expression levels of autoantibodies against tumor-associated antigens MAGEA1, VCL, TP53, and PRKCZ in the serum of a large sample of people, and the value of the four anti-tumor-associated antigen autoantibodies for the diagnosis of esophageal squamous cell carcinoma was evaluated

[0102] The indirect ELISA method was used to further detect the expression levels of the four anti-tumor-associated autoantibodies in the serum of a large sample of people, and to verify the ability of these four anti-tumor-associated autoantibodies to distinguish patients with esophageal squamous cell carcinoma from healthy controls.

[0103] 1. Experimental sample:

[0104] In this study, 126 patients with esophageal squamous cell carcinoma (esophageal squamous cell carcinoma group) and 126 healthy controls (healthy control group) were included. Among them, the serum of 126 patients with esophageal squamous cell carcinoma (esophageal squamous c...

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Abstract

The invention belongs to the technical field of medical biology, and particularly discloses a biomarker for auxiliary diagnosis of esophageal squamous carcinoma. The biomarker for diagnosing esophageal squamous cell carcinoma, provided by the invention, is at least one of autoantibodies of anti-tumor related antigens MAGEA1, VCL, TP53 and PRKCZ. The invention also provides a kit for auxiliary diagnosis of esophageal squamous carcinoma, the kit contains a reagent for detecting the biomarker, and the reagent is used for detecting the biomarker in a sample through enzyme-linked immunosorbent assay, a protein chip, immunoblotting or microfluidic immunoassay. By detecting the expression levels of autoantibodies of anti-tumor related antigens MAGEA1, VCL, TP53 and PRKCZ in human serum, esophageal squamous cell carcinoma patients and normal people can be effectively distinguished, and the kit can be used for auxiliary diagnosis of esophageal squamous cell carcinoma.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and specifically discloses an ELISA kit for auxiliary diagnosis of esophageal squamous cell carcinoma. Background technique [0002] Esophageal cancer is one of the most common malignant tumors in the world, and its morbidity and mortality are relatively high. Cancer statistics released in 2020 show that esophageal cancer has a higher incidence in Asia, especially East Asia, and men are higher than women in the regional distribution of esophageal cancer. In China, which is located in East Asia, the number of new cases of esophageal cancer in 2018 was about 324,000, accounting for about 50% of the world; the number of deaths was 301,000, accounting for about 55% of the world. The epidemic has intensified, and the prevention and treatment of esophageal cancer is urgent. According to clinical histopathological types, esophageal cancer is mainly divided into esophageal adenocarcinoma (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/543
CPCG01N33/57407G01N33/57488G01N33/543
Inventor 叶华王鹏韩卓方志豪史健翔代丽萍王科妍
Owner ZHENGZHOU UNIV
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