Synbiotic composition and preparation method and application thereof
A composition and synbiotic technology, applied in the direction of bacteria, application, and drug combination used in food preparation, to achieve the effects of improving glucose and lipid metabolism, prolonging the validity period, and promoting the survival rate
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[0039] Wherein, in some more specific implementation cases, the preparation method of the synbiotic composition includes the following steps:
[0040] S1: Lactobacillus plantarum LLY-606 was cultured in MRS medium for 12-16 hours;
[0041]S2: take the Lactobacillus plantarum LLY-606 obtained in step S1, centrifuge to get the sludge, and then dissolve it in sterile anaerobic PBS to obtain a sludge solution;
[0042] S3: Weigh the galacto-oligosaccharide according to the weight ratio, and dissolve the galacto-oligosaccharide with sterile double distilled water to form a galacto-oligosaccharide solution;
[0043] S4: Mix the bacterium slime solution obtained in step S2 with the galacto-oligosaccharide solution obtained in S3, and stir evenly to obtain a synbiotic composition.
Embodiment 1
[0046] This embodiment provides a synbiotic composition, including probiotics and prebiotics, the probiotics are Lactobacillus plantarum LLY-606, the prebiotics are galacto-oligosaccharides, and the synbiotic composition contains a dose of 1×10 5 CFU of Lactobacillus plantarum LLY-606, containing 0.01 g of galacto-oligosaccharides.
Embodiment 2
[0048] This embodiment provides a synbiotic composition, including probiotics and prebiotics, the probiotics are Lactobacillus plantarum LLY-606, the prebiotics are galacto-oligosaccharides, and the synbiotic composition contains a dose of 1×10 12 CFU of Lactobacillus plantarum LLY-606, and contains 2 g of galacto-oligosaccharides.
[0049] The preparation method of synbiotic composition, the method comprises the following steps:
[0050] S1: Lactobacillus plantarum LLY-606 was cultured in MRS medium for 12 hours;
[0051] S2: take the Lactobacillus plantarum LLY-606 obtained in step S1, centrifuge to get the sludge, and then dissolve it in sterile anaerobic PBS to obtain a sludge solution;
[0052] S3: Weigh the galacto-oligosaccharide according to the weight ratio, and dissolve the galacto-oligosaccharide with sterile double distilled water to form a galacto-oligosaccharide solution;
[0053] S4: Mix the bacterium slime solution obtained in step S2 with the galacto-oligosa...
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