Synbiotic composition and preparation method and application thereof
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A composition and synbiotic technology, applied in the direction of bacteria, application, and drug combination used in food preparation, to achieve the effects of improving glucose and lipid metabolism, prolonging the validity period, and promoting the survival rate
Pending Publication Date: 2021-12-21
BGI INST OF APPLIED AGRI +1
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Moreover, the main function of these probiotics and / or prebiotics products is to promote the establishment of a healthy micro-ecology in the body and improve the body's immunity. There are no probiotics and / or prebiotics products aimed at the healthy metabolism of the body
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preparation example Construction
[0039] Wherein, in some more specific implementation cases, the preparation method of the synbiotic composition includes the following steps:
[0040] S1: Lactobacillus plantarum LLY-606 was cultured in MRS medium for 12-16 hours;
[0041]S2: take the Lactobacillus plantarum LLY-606 obtained in step S1, centrifuge to get the sludge, and then dissolve it in sterile anaerobic PBS to obtain a sludge solution;
[0042] S3: Weigh the galacto-oligosaccharide according to the weight ratio, and dissolve the galacto-oligosaccharide with sterile double distilled water to form a galacto-oligosaccharide solution;
[0043] S4: Mix the bacterium slime solution obtained in step S2 with the galacto-oligosaccharide solution obtained in S3, and stir evenly to obtain a synbiotic composition.
Embodiment 1
[0046] This embodiment provides a synbiotic composition, including probiotics and prebiotics, the probiotics are Lactobacillus plantarum LLY-606, the prebiotics are galacto-oligosaccharides, and the synbiotic composition contains a dose of 1×10 5 CFU of Lactobacillus plantarum LLY-606, containing 0.01 g of galacto-oligosaccharides.
Embodiment 2
[0048] This embodiment provides a synbiotic composition, including probiotics and prebiotics, the probiotics are Lactobacillus plantarum LLY-606, the prebiotics are galacto-oligosaccharides, and the synbiotic composition contains a dose of 1×10 12 CFU of Lactobacillus plantarum LLY-606, and contains 2 g of galacto-oligosaccharides.
[0049] The preparation method of synbiotic composition, the method comprises the following steps:
[0050] S1: Lactobacillus plantarum LLY-606 was cultured in MRS medium for 12 hours;
[0051] S2: take the Lactobacillus plantarum LLY-606 obtained in step S1, centrifuge to get the sludge, and then dissolve it in sterile anaerobic PBS to obtain a sludge solution;
[0052] S3: Weigh the galacto-oligosaccharide according to the weight ratio, and dissolve the galacto-oligosaccharide with sterile double distilled water to form a galacto-oligosaccharide solution;
[0053] S4: Mix the bacterium slime solution obtained in step S2 with the galacto-oligosa...
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Abstract
The invention relates to a synbiotics composition and a preparation method and application thereof. The synbiotics composition is prepared from probiotics (lactobacillus plantarum LLY-606) and prebiotics (galactooligosaccharide). The inventor finds that the probiotics and the prebiotics both have the good glucolipid metabolism improving effect through research, the effect is remarkably improved after the probiotics and the prebiotics are combined for use, the synbiotics composition has an obvious synergistic effect which is specifically expressed in the aspects of obviously improving four blood lipids, improving insulin sensitivity, reducing postprandial blood sugar, reducing body weight, improving cognitive function, improving immunity or regulating intestinal flora and the like; and the probiotics and the prebiotics are reasonably proportioned, so that the efficacy is effectively enhanced, the prebiotics can effectively increase the number of the probiotics in the composition and prolong the validity period of the prebiotics, the efficacy is lasting, and the survival rate of the probiotics in intestinal tracts is promoted.
Description
technical field [0001] The invention belongs to the technical field of microbial additives, and in particular relates to a synbiotic composition and its preparation method and application. Background technique [0002] With economic development and lifestyle changes, obesity has become a serious public health problem. Modern people consume nutritious food and even excessive intake of high-fat and high-sugar "Western diet". This high-calorie dietary pattern promotes the frequent occurrence of chronic metabolic disorders such as obesity, insulin resistance and type II diabetes, triggering a series of health problems. Diabetes can cause neurophysiological and structural changes in the brain, including reduced synaptic plasticity in the hippocampus, increased levels of oxidative stress in the brain, and mitochondrial dysfunction, which can lead to a decline in cognitive learning ability and the risk of Alzheimer's disease Increase. Studies have found that prebiotic and probio...
Claims
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