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Decomposed leavening agent and application thereof

A starter and decomposing technology, applied in the field of starter, can solve problems such as environmental pollution, corrosion, secondary pollution of waste liquid, etc.

Active Publication Date: 2021-12-21
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its limitations are: the chemical absorption method needs to add a series of chemical reagents, which will cause corrosion to sewage treatment structures such as deodorization devices and various pipelines; when using strong acid or strong alkali, it is necessary to consider operational safety factors, and the waste liquid produced after absorption prone to secondary pollution
So many discarded mussel shells accumulate or go to the nearby sea area or beach, which will cause serious environmental pollution. Expanding the resource utilization of discarded mussel shells has become an urgent problem to be solved

Method used

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  • Decomposed leavening agent and application thereof
  • Decomposed leavening agent and application thereof
  • Decomposed leavening agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Screening and Identification of Pseudomonas sp. LZ4-5

[0038] 1.1 Screening method

[0039] (1) Sample collection

[0040] Sediment sample collection: Laizhou Bay collection, with 0.05m 2The stainless steel GrayO'Hara box-type mud sampler was used to collect seawater sediment samples in selected survey stations, to remove samples that may be contaminated on the surface, and to collect sediments approximately below the seabed surface with a sterile 60mL syringe (no luer end). 5cm samples, immediately transferred to a sterile ziplock bag for low temperature storage. The samples were stored in dry ice at low temperature on the sampling ship, and the sulfur-oxidizing bacteria were isolated after returning to the laboratory.

[0041] (2) Isolation, purification and preservation of sulfur oxidizing bacteria

[0042] Screening culture: take 1g of sediment sample, add sterilized seawater, dilute 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 , and then draw 100ml of the dilute...

Embodiment 2

[0051] 2.1 Culture method of strain

[0052] 1) Use 2216E medium to cultivate (purchase finished medium) LZ4-5 strain, and the culture condition is 30°C.

[0053] 2.2 Analysis of intermediate metabolites in the degradation process of sodium sulfide

[0054] Transfer the strain to be tested to a plate with sodium thiosulfate as the substrate;

[0055] Inoculate the purified degrading strain with 5% inoculum in the inorganic salt basic culture medium with thiosulfate as the substrate to verify whether it has degradation ability. If turbidity occurs, the bacterial solution can be used in the next step operate.

[0056] Put 15mL of bacterial solution into a conical flask filled with 150mL of culture medium (when the bacterial solution OD600nm=0.22, the concentration of the bacterial solution is about 0.44*10^9cfu / ml), and place it on a shaker at 30°C and 160r / min Cultivate, take samples regularly during the cultivation process (take samples from the shaker every 12 hours and pr...

Embodiment 3

[0072] 3.1 Ratio of fermentation raw materials

[0073] The list of raw materials is shown in Table 3-1 below.

[0074] Table 3-1 List of raw materials for aerobic fermentation of shell powder

[0075]

[0076] Other supporting conditions required for aerobic fermentation of sludge and mussel shells

[0077] ①The fermentation pile is 13 meters long, 4 meters wide, and 1.6±0.1 meters high, about 60m 3 Fermentation volume, the material weight of a single pile after mixing is 38-40 tons;

[0078] ② The fermentation air duct uses 2 air ducts, and the ventilation length of the 2 air ducts is 10 meters;

[0079] ③Use paint to draw the boundary of the fermentation area, confirm the distance between the piles, and the inside of the paint line is the actual length of the fermentation pile;

[0080] ④ The power demand of the control system is three-phase 380V, and the maximum power of a single unit is 3.7KW.

[0081] Fermentation material configuration

[0082] 1) Use a throwin...

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Abstract

The invention relates to the technical field of leavening agents, and discloses Pseudomonas sp. LZ4-5 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.21761. The invention also discloses a decomposed leavening agent, and the decomposition leavening agent contains the pseudomonas sp. LZ4-5. The invention also discloses an application of the Pseudomonas sp. LZ4-5 in preparation of a sludge and mussel shell combined film-mulching aerobic fermentation decomposed fertilizer. The invention further discloses the sludge and mussel shell combined film-mulching aerobic fermentation decomposed fertilizer prepared by applying the decomposed leavening agent. A proper amount of the decomposed leavening agent is added into a total fermentation material obtained through sludge and mussel shell combined film-mulching and aerobic fermentation decomposition, organic matter and mussel shell powder in the sludge can be effectively degraded, and the decomposed organic fertilizer completely meeting the index requirements of products such as organic fertilizer is obtained; and meanwhile, the problem of resource utilization of Zichan city sludge and the problem of ecological environment of Zichan city mussel shells are solved, and a new method is provided for resource utilization of mussel shells.

Description

technical field [0001] The invention relates to the technical field of starters, in particular to a decomposed starter and its application. Background technique [0002] With my country's increasing emphasis on environmental protection and huge investment in sewage treatment, urban sewage has been effectively collected and treated, and a large amount of municipal sludge has been produced in the process. Sludge contains pathogens, parasites, heavy metals, refractory organic matter, carcinogenic organic matter and other toxic substances, which can cause serious environmental pollution. At the same time, urban sludge is rich in N, P, K, Ca and other elements. The sludge after harmless treatment can improve the physical and chemical properties of the soil, increase the yield of crops, and has certain agricultural application value. The treatment and disposal of sludge includes sludge treatment and sludge disposal, with the purpose of harmless treatment of sludge and avoiding ha...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C05F17/20C05F17/80C05G1/00C12R1/38C12R1/125
CPCC12N1/20C12N1/16C05F17/20C05F17/80C05C9/00C05F1/005C05F5/00C05F5/002C05F7/00Y02W30/40
Inventor 刘瑞志陈唯李子成谷秀张宇轩孟庆佳王丽平
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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