Facial mask essence containing hyaluronic acid and preparation method thereof
A technology of hyaluronic acid and essence, which can be used in medical preparations containing active ingredients, preparations for skin care, pharmaceutical formulas, etc., can solve problems such as the decrease in the stability of plant antimicrobial peptides
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Embodiment 1
[0024] The formula composition and preparation method of embodiment 1 facial mask essence
[0025] The preparation methods of the facial mask essence samples in this application are all the same and only have different formulations. The formulations of all samples are shown in Table 1:
[0026] Table 1 Formula table of facial mask essence sample
[0027]
[0028] Note: "-" means that no certain substance has been added; among them, the essence added with plant defensins precipitated during the test, because plant defensins have strong cations, hyaluronic acid has negative charges, and the electrostatic interaction between the two Sincerely.
[0029] Among them, the facial mask essence of the present invention is the No. 3 sample formula, and the preparation method is as follows: 73.04% deionized water, 0.25% reduced glutathione, 0.5% hyaluronic acid, 0.3% plant defensin, 0.1% penetrating Add pentasodium acid, 0.4% trehalose and other auxiliary materials in turn into the b...
Embodiment 2
[0031] Embodiment 2 antibacterial and anticorrosion performance detection
[0032] Preparation of mixed bacterial solution for testing: Inoculate various strains in a suitable medium, culture bacteria at 37°C for 48 hours, and fungi at 28°C for 72 hours. Select an appropriate amount of colonies in sterilized saline to make a certain concentration of mixed bacteria (the number of colonies is 10 8 CFU / mL or so) or mixed fungal suspension (the number of colonies is less than 10 7 CFU / mL or so), store at 4°C for later use.
[0033] Method for testing the effectiveness of preservatives using a one-time microbiological challenge test. Weigh 100 mL of No. 1-3 sample essence in Example 1, add 1 mL of mixed bacteria or mixed fungal suspension, and then fully mix. Guarantee that in the sample added to the bacterial suspension, the bacterial content per milliliter of the sample is at 10 6 About CFU / mL, in the sample added to the fungal suspension, the bacterial content per gram of sa...
Embodiment 3
[0040] Embodiment 3 detects the transdermal absorption of GSH in the isolated skin
[0041] 3.1 Standard curve establishment (HPLC method)
[0042] Chromatographic conditions: Chromatographic column: Agilent Zorbax SB-Aq, mobile phase is binary gradient mobile phase system A: 0.025% potassium dihydrogen phosphate (pH3.8); B: methanol, flow rate 1.0mL / min, injection volume 20μL, column temperature 30°C, detection wavelength: 210nm.
[0043] Preparation of standard solution: Accurately weigh 10mg of GSH, put it in a 10mL measuring bottle, dissolve the blank skin receiving solution and dilute to the mark, shake well, and prepare a stock solution. Precisely measure 0.1, 0.5, 2.5, 5.0, 10.0mL stock solutions respectively, place them in 10mL measuring bottles, and use blank skin receiving solution to make up volume to obtain 1.0, 5.0, 25.0, 50.0, 100.0ug / mL reference substance solutions.
[0044] Standard curve establishment: Accurately measure 1 mL of the above-mentioned referenc...
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