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Polyketide synthase Preu3-delta CMeT and application thereof in preparation of orsellinic acid

A technology of polyketide synthase and moss acid, applied in the fields of molecular biology and biochemistry, can solve the problems of low yield, limited application and high price, and achieve the effect of high yield and simple process

Active Publication Date: 2021-12-24
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, commercially available orsic acid (CAS: 480-64-8) is produced by chemical synthesis, and the price is about 1,000 yuan / 100mg. The output is low and the price is high, so its application in food, medicine and other fields is restricted. limit

Method used

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  • Polyketide synthase Preu3-delta CMeT and application thereof in preparation of orsellinic acid
  • Polyketide synthase Preu3-delta CMeT and application thereof in preparation of orsellinic acid
  • Polyketide synthase Preu3-delta CMeT and application thereof in preparation of orsellinic acid

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Embodiment Construction

[0036] The present invention will be further described in detail below in conjunction with the examples.

[0037] Those skilled in the art will understand that the following examples are only for illustrating the present invention and should not be considered as limiting the scope of the present invention. If no specific technique or condition is indicated in the examples, it shall be carried out according to the technique or condition described in the literature in this field or according to the product specification. The materials or equipment used are not indicated by the manufacturer, and they are all conventional products that can be obtained through purchase.

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Abstract

The invention relates to a polyketide synthase Preu3-delta CMeT and application thereof in preparation of orsellinic acid, and belongs to the technical field of molecular biology and biochemistry. The amino acid sequence of the polyketide synthase Preu3-delta CMeT is as shown in SEQ ID NO.1, and the polyketide synthase Preu3-delta CMeT is constructed by taking polyketide synthase Preu3 derived from phorbia fungi as a material and knocking out a CMeT structural domain of the polyketide synthase Preu3-delta CMeT on the basis of a combined biosynthesis technology; the polyketide synthase Preu3-delta CMeT is transferred into saccharomyces cerevisiae to successfully obtain a mutant strain capable of efficiently producing the orsellinic acid; and the prepared orsellinic acid is taken as an object, the inhibitory activity of the orsellinic acid on clinical drug-resistant bacteria and crop pathogenic fungi is researched, and the orsellinic acid is found to have a very strong antagonistic effect on carbapenem-resistant pseudomonas aeruginosa, and the MIC is 12.5 [mu]g / mL. The polyketide synthase Preu3-delta CMeT greatly enriches the production sources of the orsellinic acid, and has important scientific value and application prospect for expanding the derivatization approach of the orsellinic acid and researching and developing a novel antibacterial agent.

Description

technical field [0001] The invention belongs to the technical field of molecular biology and biochemistry, and specifically relates to a novel "non-natural" polyketide synthase Preu3-ΔCMeT and its application in preparing orsinic acid. Background technique [0002] Fungal polyketides ( p oly k etide s , PKs) is a large class of important natural product groups with diverse chemical structures and biological activities, and is a high-quality resource library for the discovery of new drug lead compounds. Representative fungal PKs include lovastatin for lowering cholesterol, griseofulvin for fungi, rifamycin for bacteria, mycophenolic acid for immunosuppression, Actin and angiogenesis inhibitor cytochalasin E (cytochalasin E), etc. The key enzyme of PKs biosynthesis - fungal polyketide synthase ( p oly k etide s ynthase, PKS) is an iterative (iterative) type I PKS, and its domain usually includes: the initiation unit ACP transacylase ( s tarter unit A CP t ransacylase...

Claims

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Application Information

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IPC IPC(8): C12N9/10C12N15/54C12N15/81C12N1/19C12P7/42C07C51/42A01N37/40C07C65/03A01P3/00A01P1/00C12R1/865
CPCC12N9/1029C12N15/81C12P7/42C07C51/42A01N37/40C12N2800/102C07C65/03Y02A50/30
Inventor 刘庆培杨小龙张丹徐瑶
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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