Rice BC20 mutant gene, recombinant vector, transformant and application of rice BC20 mutant gene, recombinant vector and transformant in preparation of rice brittle stem mutant

Abstract

The invention provides a rice BC20 mutant gene, a recombinant vector, a transformant and application of the rice BC20 mutant gene, the recombinant vector and the transformant in preparation of a rice brittle stem mutant. The mutant gene comprises a mutant bc20-1, wherein the nucleotide sequence of the mutant bc20-1 is as shown in SEQ ID NO: 5; and a mutant bc20-2, wherein the nucleotide sequence of the mutant bc20-2 is as shown in SEQ ID NO: 6. The mutant bc20-1 and bc20-2 plants show that the plants are short and small, the tillering is reduced, the mechanical strength is reduced, the stalks are easy to break, the rice grain shape is reduced, and the like. The further observation of wild type and mutant bc20-1 and bc20-2 plant stem thick-wall cells through a transmission electron microscope finds that the cell wall thickness of the mutant stem thick-wall cells is obviously thinner than that of the wild type plant stem thick-wall cells. Straw of the mutants is easy to smash, and the mutant has important significance in agricultural production, renewable resource utilization and the like in China.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a rice BC20 mutant gene, a recombinant vector, a transformant and the application thereof in preparing a rice brittle stem mutant. Background technique [0002] The cell wall is a natural nanoscale network structure, mainly composed of polysaccharide polymers such as cellulose, hemicellulose and pectin, lignin and a small amount of glycoproteins and minerals. This complex structure envelops plant cells and provides them with different shapes, sizes and physicochemical properties that allow them to function in different organs and stages of development. At the same time, the cell wall has a variety of biological functions, including determining plant morphogenesis and structure, providing mechanical support, transferring water and nutrients, and resisting biotic and abiotic stresses, etc. In addition, plant cell walls can also be used in animal feed, building materials, biofuels, tex...

AI Technical Summary

Problems solved by technology

For example, the BC1 gene encodes a glycosylphosphatidylinositol-anchored protein, which is related to the synthesis of cell walls, and its mutation leads to abnormal cell swelling, decreased cellulose content, and increased lignin content; BC3 gene encodes a membrane-associated dynein, which may be involved in Disrupts the endocytic pathway and post-Golgi membrane trafficking, and its mutations interfere with normal cellulose biosynthesis and membrane trafficking of secondary cell walls

Images