Unlock instant, AI-driven research and patent intelligence for your innovation.

Helicobacter pylori VOC marker and application and detection system thereof

A technology of Helicobacter pylori and detection system, applied in the field of its application and detection system, Helicobacter pylori VOC marker, achieves the effects of accurate and reliable diagnosis, low cost, easy storage and transportation

Inactive Publication Date: 2021-12-31
SHANGHAI JIAO TONG UNIV
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no relevant research on volatile organic compounds in exhaled breath as markers of Helicobacter pylori infection. Therefore, it is urgent to develop an analysis method for specific markers in exhaled breath of Helicobacter pylori infected patients, and to screen out the pylori with significant differences. Helicobacter infection markers and design of corresponding detection methods

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Helicobacter pylori VOC marker and application and detection system thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Collection of breath samples

[0026] The invention screens out the VOC markers in the exhaled breath by analyzing the exhaled breath of positive and negative volunteers infected with Helicobacter pylori. None of the 30 volunteers (20 patients infected with Helicobacter pylori and 10 normal people) who provided exhalation suffered from other malignant tumors, blood diseases or metabolic system diseases. 20 patients with Helicobacter pylori infection were confirmed to be infected with Helicobacter pylori after positive C14 breath test and positive bacterial culture of gastric mucosa samples taken by gastroscopy in advance. They did not take antibiotics or other interfering drugs within one month of gas collection.

[0027] Breath samples were analyzed for each subject, using a total of 30 breath samples. Before the sample collection, each volunteer should maintain a natural and peaceful mood within 48 hours, and must not smoke or drink within 24 hours. Information such...

Embodiment 2

[0029] Examination of breath samples

[0030] The collected breath of volunteers was stored in a 4-degree refrigerator, and enriched within 2 hours. The enrichment tube was an adsorption tube that had been inertized inside. The adsorption tube was aged at 330 degrees Celsius for 30 minutes before use, and a high-purity nitrogen gas flow was continuously passed through. After the desorption tube is cooled, one end is connected to the air bag through a polytetrafluoroethylene tube, and the other end is connected to a high-precision air pump so that the direction of the air flow is consistent with the direction of the arrow marked on the adsorption tube. The flow rate of ml / min is passed through the adsorption tube, and the pumping time is 15 minutes, that is, 750 ml of gas is pre-concentrated in the enrichment tube. After enrichment, both ends are sealed with brass caps with Teflon gaskets. The adsorption tube enriched with breath samples was desorbed at a high temperature of 2...

Embodiment 3

[0033] Screening and verification of markers in breath samples

[0034] The compounds separated by the chromatographic column are detected by mass spectrometry, and the detected substances are preliminarily qualified using the NIST14 library that comes with the mass spectrometer, and the substances with a similarity of more than 90% are quantified using the peak area percentage.

[0035] Gas chromatography-mass spectrograms of exhaled breath from patients with Helicobacter pylori infection and healthy subjects and the corresponding markers of Helicobacter pylori infection such as figure 1 and shown in Table 1.

[0036] Table 1 Gastric cancer markers in the breath of Helicobacter pylori positive and negative volunteers

[0037] peak number Compound name CAS number 1 n-Hexane 110-54-3 2 ethyl acetate 141-78-6 3 Isobutanol 75-65-0 4 ethanol 64-17-5 5 Toluene 108-88-3 6 1-propanol 71-23-8 7 n-Hexanal 66-25-1 8 O...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a helicobacter pylori VOC marker as well as application and a detection system thereof. The marker is a combination of multiple or all of the following volatile organic compounds: n-hexane, ethyl acetate, isobutanol, ethanol, toluene, 1-propanol, n-hexanal, o-xylene, pyridine, nonanal, formamide and cetyl-1, 4-lactone. A diagnosis system is used for diagnosing helicobacter pylori infection by detecting the content of the helicobacter pylori VOC marker in exhaled air of a testee. Various volatile organic compounds in exhaled air are used as markers for non-label diagnosis of helicobacter pylori infection, and the application prospect in screening and timely treatment of helicobacter pylori is wide.

Description

technical field [0001] The invention relates to the technical field of non-marker detection of Helicobacter pylori, in particular to a Helicobacter pylori VOC marker, its application and a detection system. Background technique [0002] Helicobacter pylori is a common bacterial pathogen that lives in the pyloric part of the human stomach. Studies have shown that Helicobacter pylori infection can induce chronic gastritis, lead to gastric ulcer and gastric atrophy, and even develop gastric cancer in severe cases. Therefore, timely detection of Helicobacter pylori patients can effectively reduce the possibility of further deterioration and even development of gastric cancer. Volatile organic compounds (VOCs) have been shown to be a source of diagnostic information for many local and systemic diseases, such as cancer, liver disease, and diabetes. Disease-related specific compounds are produced. Some VOCs in blood can pass through extracellular or paracellular mechanism through...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/72
Inventor 崔大祥薛翠丽金涵张禹娜
Owner SHANGHAI JIAO TONG UNIV