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Medicine for killing nematodes by poisoning

A technology for poisoning nematodes and drugs, applied in the directions of nematicides, biocides, biocides, etc., can solve the problems of excessive pesticide residues, human and animal poisoning, aggravating environmental pollution, etc. The effect of the nematicidal effect

Inactive Publication Date: 2022-01-04
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, chemical control is the most widely used in production, but its disadvantage is that pesticide residues in agricultural products exceed the standard, causing poisonous effects on humans and animals, and they can destroy the ozone layer (such as methyl bromide) and aggravate environmental pollution

Method used

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  • Medicine for killing nematodes by poisoning
  • Medicine for killing nematodes by poisoning

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Medicine preparation: get 10mg methyl eugenol (commercially available methyl eugenol standard product) and add 1mL volume fraction and be 5% Tween 80 aqueous solution, vortex stir with vortex agitator, be mixed with the concentration that is 10mg / mL of methyl eugenol stock solution.

[0034] (2) Dilute the methyl eugenol mother liquor prepared by step (1) with a solvent, and prepare a methyl eugenol solution with a concentration of 200mg / L, 400mg / L, 600mg / L, and 800mg / L respectively.

[0035] The preparation method of the solvent is as follows: take 1.18g of KCl and 1.5g of NaCl and add distilled water to make up to 500mL, autoclave at 121°C, place at room temperature, take 100mL and add 5mL of Tween 80 to mix evenly to obtain the solvent.

[0036] (3) Preparation of nematodes: wash the Caenorhabditis elegans adults in NGM medium with M9 buffer solution and place them in a 1.5mL centrifuge tube, centrifuge at 4000rpm to save the precipitate, wash 3 times, then add ...

Embodiment 2

[0047] (1) Medicine preparation: get 10mg methyl eugenol (commercially available methyl eugenol standard product) and add 1mL volume fraction and be 5% Tween 80 aqueous solution, vortex stir with vortex agitator, be mixed with the concentration that is 10mg / mL of methyl eugenol stock solution.

[0048] (2) Dilute the methyl eugenol mother liquor prepared in step (1) with a solvent, and prepare methyl eugenol solutions with concentrations of 42.3 mg / L, 4.23 mg / L, and 0.423 mg / L respectively.

[0049] The preparation method of the solvent is as follows: take 1.18g of KCl and 1.5g of NaCl and add distilled water to make up to 500mL, autoclave at 121°C for 20min, take 100mL and add 5mL of Tween 80 to mix evenly to obtain the solvent.

[0050] (3) Preparation of nematodes: wash the Caenorhabditis elegans adults in the plate with M9 buffer solution and place them in a 1.5mL centrifuge tube, centrifuge at 4000rpm to save the precipitate, wash 3 times, then add 1mL of lysate (1mL vol...

Embodiment 3

[0056] (1) Medicine preparation: get 10mg methyl eugenol (commercially available methyl eugenol standard product) and add 1mL volume fraction and be 5% Tween 80 aqueous solution, vortex stir with vortex agitator, be mixed with the concentration that is 10mg / mL of methyl eugenol stock solution.

[0057] (2) Dilute the methyl eugenol mother liquor prepared in step (1) with a solvent, and prepare methyl eugenol solutions with concentrations of 42.3 mg / L, 4.23 mg / L, and 0.423 mg / L respectively.

[0058] The preparation method of the solvent is as follows: take 1.18g of KCl and 1.5g of NaCl and add distilled water to make up to 500mL, autoclave at 121°C for 20min, take 100mL and add 5mL of Tween 80 to mix evenly to obtain the solvent.

[0059] (3) Preparation of nematodes: wash the Caenorhabditis elegans adults in the plate with M9 buffer solution and place them in a 1.5mL centrifuge tube, centrifuge at 4000rpm to save the precipitate, wash 3 times, then add 1mL of lysate (1mL vol...

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Abstract

The invention discloses a medicine for killing nematodes by poisoning, and belongs to the technical field of nematode prevention and control. The main component of the medicine for killing the nematodes by poisoning is methyleugenol which is a natural compound extracted from plants, has a good poisoning and killing effect on the caenorhabditis elegans, inhibits the service life, egg laying number and acetylcholin esterase activity of the caenorhabditis elegans, and has the characteristics of low toxicity, low residue and safety to human and environment. The medicine can be applied to prevention and treatment of nematode diseases of medicinal plants such as ginseng, American ginseng and pseudo-ginseng, and can also be applied to prevention and treatment of nematode diseases of field crops such as wheat, corn, rice and potatoes and prevention and treatment of plant nematode diseases of vegetable crops such as Chinese cabbage, tomato, cucumber and the like. The medicine better solves the problems of high toxicity, environmental pollution, residues in crops and the like of the existing nematicide, and improves the safety of agricultural production.

Description

technical field [0001] The invention relates to the technical field of nematode control, in particular to a medicine for killing nematodes. Background technique [0002] Methyl eugenol mainly exists in the extracts of Chinese herbal medicines such as Herba Asarum and Nutmeg, and is a known compound that can be purchased from the market. The structural formula is as shown in formula (1): [0003] [0004] Plant parasitic nematodes are one of the important pathogens in agricultural production, and they are also a highly specialized type of plant-fixed endoparasitic nematodes. It usually causes the root of the host plant to be swollen, tumor-like or root-knot-like and cause the diseased root to rot. The above-ground part shows that the plant is short, the leaves are yellow, wilting and dead, which seriously reduces the yield and quality of crops. Nematode diseases cause economic losses to agricultural production of more than 100 billion U.S. dollars per year worldwide. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N31/14A01P5/00
CPCA01N31/14
Inventor 王国健张瑞杨佳彤王英平焉学倩李伟
Owner JILIN AGRICULTURAL UNIV
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