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Bone marrow mesenchymal stem cell injection as well as preparation method and application thereof

A bone marrow mesenchymal and stem cell technology, applied in the field of stem cells, can solve the problems of affecting the activity of bone marrow mesenchymal stem cells, poor biological activity of stem cells, and inability to guarantee safety, etc., to facilitate commercial production, improve viability, and improve bone quality. The effect of hypoplastic symptoms

Pending Publication Date: 2022-01-04
王啸远
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the in vitro preservation of transplanted exogenous bone marrow mesenchymal stem cells, it is easy to cause exogenous damage due to various improper preservation, and the cell survival rate is low. On the other hand, the safety after injection into the human body cannot be guaranteed, which eventually leads to poor biological activity of stem cells after injection into the body, which greatly hinders its clinical application. The efficiency of mesenchymal stem cell transplantation is one of the problems to be solved to improve the clinical application effect of bone marrow mesenchymal stem cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A bone marrow mesenchymal stem cell injection, each 100mL injection contains: bone marrow mesenchymal stem cells 5 × 10 6 1, isopimaric acid 0.3g, Chizhi polysaccharide 7mg, iRGD 0.7μg, compound amino acid 8g, sodium citrate 0.5g, sodium chloride 1.5g, the balance is deionized water; bone marrow mesenchymal stem cells are subcultured P5 generation Bone marrow mesenchymal stem cells in the logarithmic growth phase; the compound amino acid is composed of L-proline, L-serine, L-alanine, and L-arginine in a mass ratio of 1:0.5:0.8:1 .

[0028] The preparation method of the above bone marrow mesenchymal stem cell injection comprises the following steps:

[0029] (1) Take sodium chloride and add it to deionized water. After it is completely dissolved, add isopimaric acid, red sesame polysaccharide, iRGD, sodium citrate, and compound amino acids, mix evenly, and then use a filter with a pore size of 0.22 μm to filter and sterilize to obtain a mixed solution ;

[0030] (2) A...

Embodiment 2

[0032] A bone marrow mesenchymal stem cell injection, each 100mL injection contains: bone marrow mesenchymal stem cells 2 × 10 6 1, isopimaric acid 0.2g, Chizhi polysaccharide 5mg, iRGD 0.5μg, compound amino acid 5g, sodium citrate 0.3g, sodium chloride 1.2g, the balance is deionized water; bone marrow mesenchymal stem cells are subcultured P3 generation Bone marrow mesenchymal stem cells in the logarithmic growth phase; the compound amino acid is L-tryptophan, L-leucine, L-methionine, L-isoleucine, L-phenylalanine, glycine at a ratio of 0.3:0.5 :0.2:0.8:0.5:1 mass ratio composition.

[0033] The preparation method of the above-mentioned bone marrow mesenchymal stem cell injection is the same as that in Example 1.

Embodiment 3

[0035] A bone marrow mesenchymal stem cell injection, each 100mL injection contains: bone marrow mesenchymal stem cells 8 × 10 6 1, isopimaric acid 0.5g, Chizhi polysaccharide 10mg, iRGD 0.9μg, compound amino acid 10g, sodium citrate 0.7g, sodium chloride 1.8g, the balance is deionized water; bone marrow mesenchymal stem cells are subcultured P8 Bone marrow mesenchymal stem cells in the logarithmic growth phase; the compound amino acid is L-arginine, L-histidine, and L-tryptophan mixed in a mass ratio of 2:1:2.

[0036] The preparation method of the above-mentioned bone marrow mesenchymal stem cell injection is the same as that in Example 1.

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Abstract

The invention discloses a bone marrow mesenchymal stem cell injection. Every 100 mL of the injection contains 2-8*10<6> bone marrow mesenchymal stem cells, 0.2-0.5 g of isopimaric acid, 5-10 mg of ganoderma lucidum polysaccharide, 0.5-0.9 [mu]g of iRGD, 5-10 g of compound amino acid, 0.3-0.7 g of sodium citrate, 1.2-1.8 g of sodium chloride and the balance of deionized water. According to the injection provided by the invention, the isopimaric acid, ganoderma lucidum polysaccharide, iRGD and other components are added into the injection, so that the activity of mesenchymal stem cells in the injection is effectively maintained, and the survival rate of the cells is improved. The invention further provides a preparation method of the bone marrow mesenchymal stem cell injection, the operation is simple and convenient, the implementation is easy, and the commercialized production of the injection is convenient. The invention also provides an application of the bone marrow mesenchymal stem cell injection in a medicine for treating osteoporosis, the injection provided by the invention improves the activity of the bone marrow mesenchymal stem cells before transplantation, ensures that the cells with enough activity and differentiation ability play a role after transplantation, and effectively relieves the symptom of osteoporosis.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to a bone marrow mesenchymal stem cell injection and a preparation method and application thereof. Background technique [0002] Mesenchymal stem cells (MSCs) are important members of the stem cell family, derived from the mesoderm and ectoderm in the early stages of development. MSCs have the characteristics of self-replication, self-renewal, multi-directional differentiation potential, hematopoietic support and immune regulation. In a specific in vitro differentiation environment, it can be induced to differentiate into various tissue cells such as bone, cartilage, and fat, and is considered to be one of the most promising seed cells for cell therapy technology. [0003] Bone marrow mesenchymal stem cells (BMSCs) are mesenchymal stem cells derived from bone marrow. Bone marrow mesenchymal stem cells are easy to isolate and culture, have weak immune rejection, and have strong self-renewa...

Claims

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Application Information

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IPC IPC(8): A61K9/08A61K35/28A61K47/36A61K47/12A61K47/18A61P19/10
CPCA61K9/08A61K9/0019A61K35/28A61K47/36A61K47/12A61K47/18A61P19/10
Inventor 王啸远李毫
Owner 王啸远
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