Bone marrow mesenchymal stem cell injection as well as preparation method and application thereof

A bone marrow mesenchymal and stem cell technology, applied in the field of stem cells, can solve the problems of affecting the activity of bone marrow mesenchymal stem cells, poor biological activity of stem cells, and inability to guarantee safety, etc., to facilitate commercial production, improve viability, and improve bone quality. The effect of hypoplastic symptoms

Pending Publication Date: 2022-01-04
王啸远
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the in vitro preservation of transplanted exogenous bone marrow mesenchymal stem cells, it is easy to cause exogenous damage due to various improper preservation, and the cell survival rate is low. On the other hand, the safety after injection into the human body cannot be guaranteed, which e

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0026] Example 1

[0027] Inter bone marrow mesenchymal stem cell injection, each injection containing 100mL: Mesenchymal Stem Cells 5 × 10 6 A, isopimaric acid 0.3g, Ganoderma lucidum polysaccharides 7mg, iRGD 0.7μg, an amino acid compound 8g, sodium citrate 0.5g, NaCl 1.5g, balance deionized water; mesenchymal stem cells are subcultured on behalf of P5 bone marrow in the logarithmic growth phase mesenchymal stem cells; the amino acid compound is L- proline, L- serine, L- alanine, L- arginine three amino acid of 1: 0.5: 0.8: 1 mass ratio of composition .

[0028] Among the above-described method for preparing bone marrow mesenchymal stem cell injection, comprising the steps of:

[0029] (1) Sodium chloride was added to take the deionized water was added until complete dissolution isopimaric acid, Ganoderma lucidum polysaccharides, of iRGD, sodium citrate, complex amino acid after mixing of pore size 0.22μm filter sterilized by filtration, to give a mixed solution ;

[0030] Mixed...

Example Embodiment

[0031] Example 2

[0032] Inter bone marrow mesenchymal stem cell injection, each injection containing 100mL: bone marrow mesenchymal stem cells 2 × 10 6 A, isopimaric acid 0.2g, Ganoderma lucidum polysaccharides 5mg, iRGD 0.5μg, an amino acid compound 5g, sodium citrate 0.3g, NaCl 1.2g, balance deionized water; mesenchymal stem cells are subcultured P3 of generations bone marrow in the logarithmic growth phase mesenchymal stem cells; compound amino acid is L- tryptophan, L- leucine, L- methionine, L- isoleucine, L- phenylalanine, glycine 0.3: 0.5 : 0.2: 0.8: 0.5: 1 mass ratio of the composition.

[0033] Among the above-described method for preparing bone marrow mesenchymal stem cell injection the same as in Example 1.

Example Embodiment

[0034] Example 3

[0035] Inter bone marrow mesenchymal stem cell injection, each injection containing 100mL: Mesenchymal Stem Cells 8 × 10 6 A, isopimaric acid 0.5g, Ganoderma lucidum polysaccharides 10mg, iRGD 0.9μg, an amino acid compound 10g, sodium citrate 0.7 g, NaCl 1.8g, balance deionized water; mesenchymal stem cells are subcultured on behalf of P8 bone marrow in the logarithmic growth phase mesenchymal stem cells; the amino acid compound is L- arginine, L- histidine, L- tryptophan to 2: 1: 2 by mass ratio.

[0036] Among the above-described method for preparing bone marrow mesenchymal stem cell injection the same as in Example 1.

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PUM

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Abstract

The invention discloses a bone marrow mesenchymal stem cell injection. Every 100 mL of the injection contains 2-8*10<6> bone marrow mesenchymal stem cells, 0.2-0.5 g of isopimaric acid, 5-10 mg of ganoderma lucidum polysaccharide, 0.5-0.9 [mu]g of iRGD, 5-10 g of compound amino acid, 0.3-0.7 g of sodium citrate, 1.2-1.8 g of sodium chloride and the balance of deionized water. According to the injection provided by the invention, the isopimaric acid, ganoderma lucidum polysaccharide, iRGD and other components are added into the injection, so that the activity of mesenchymal stem cells in the injection is effectively maintained, and the survival rate of the cells is improved. The invention further provides a preparation method of the bone marrow mesenchymal stem cell injection, the operation is simple and convenient, the implementation is easy, and the commercialized production of the injection is convenient. The invention also provides an application of the bone marrow mesenchymal stem cell injection in a medicine for treating osteoporosis, the injection provided by the invention improves the activity of the bone marrow mesenchymal stem cells before transplantation, ensures that the cells with enough activity and differentiation ability play a role after transplantation, and effectively relieves the symptom of osteoporosis.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to a bone marrow mesenchymal stem cell injection and a preparation method and application thereof. Background technique [0002] Mesenchymal stem cells (MSCs) are important members of the stem cell family, derived from the mesoderm and ectoderm in the early stages of development. MSCs have the characteristics of self-replication, self-renewal, multi-directional differentiation potential, hematopoietic support and immune regulation. In a specific in vitro differentiation environment, it can be induced to differentiate into various tissue cells such as bone, cartilage, and fat, and is considered to be one of the most promising seed cells for cell therapy technology. [0003] Bone marrow mesenchymal stem cells (BMSCs) are mesenchymal stem cells derived from bone marrow. Bone marrow mesenchymal stem cells are easy to isolate and culture, have weak immune rejection, and have strong self-renewa...

Claims

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Application Information

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IPC IPC(8): A61K9/08A61K35/28A61K47/36A61K47/12A61K47/18A61P19/10
CPCA61K9/08A61K9/0019A61K35/28A61K47/36A61K47/12A61K47/18A61P19/10
Inventor 王啸远李毫
Owner 王啸远
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