Siphon type total homocysteine test paper and manufacturing method thereof

Abstract

The invention discloses siphon type total homocysteine test paper and a manufacturing method thereof. The test paper structurally comprises a hydrophilic cover sheet, a PET double-faced adhesive tape blood guide groove, a polyester gauze, a blood suction pad, a blood filtering film, a color developing film, a PET interlayer, a double-faced adhesive tape pad and a PET bottom plate in sequence from top to bottom. According to the invention, a test can be carried out by using fingertip blood, the siphon type design of a siphon sample adding port enables the test speed to be higher and time to be saved, the overall design saves space, sampling is convenient and small in amount, a patient does not feel pain, sampling is not depended on professionals, the test result covers all forms of homocysteine, the result sensitivity is higher and more accurate, the problem that the sum of all forms of homocysteine cannot be detected when homocysteine is detected at present is perfectly solved, the problem of interference of a dissociation agent pair is solved, and the test paper meets and is suitable for rapid and convenient detection requirements of inspection places of primary hospitals.

Description

technical field [0001] The invention relates to the technical field of test papers, in particular to a siphon type total homocysteine ​​test paper and a manufacturing method thereof. Background technique [0002] Homocysteine ​​(HCY) is a sulfur-containing amino acid and an intermediate product of methionine metabolism. It itself does not participate in protein synthesis. Homocysteine ​​cannot be synthesized in the body, but can only be converted from methionine. Human body Methionine cannot be synthesized and must be supplied by food. The main factors affecting the level of homocysteine ​​are genetics and food nutrition deficiency. Vitamin B6, vitamin B12, and folic acid are needed in the metabolism of homocysteine. If vitamin B12, vitamin B12, and B6. If the concentration of folic acid is too low, the concentration of homocysteine ​​in the blood will increase, which is common in atherosclerotic vascular disease, stroke, rheumatoid arthritis, various cancers, etc., and the ...

AI Technical Summary

Problems solved by technology

[0003] In response to the demand for rapid and convenient detection of homocysteine, none of the existing test methods reflects the dissociation process of bound HCY, so only the free state of HCY in the blood is measured, and the content of tHCY cannot be detected, but Free HCY only accounts for 1% of tHCY, which is too small to feed back more useful information in terms of results, and is of little significance for clinical diagnosis;

[0004] The patent (application number CN202010147017.5) discloses a dry-type homocysteine ​​test card and its application. This method solves the technical problem that the previous detection method cannot measure tHCY. The dissociating agent makes all the bound HCY become free. After the dissociation is completed, add enzymes to finally generate hydrogen peroxide. Under the catalysis of peroxidase, Trinder's chromogenic substrate is oxidized by hydrogen peroxide to display color changes, although This method dissociates the conjugated homocysteine, but the dissociating agent contains reducing properties and will react with hydrogen peroxide, resulting in a decrease in the concentration of the final product, while the amount of hydrogen peroxide generated is comparable to that of homocysteine Acid is proportional, so the reduction of hydrogen peroxide content will affect the detection results, the accuracy will be reduced, and the operation is cumbersome and error-prone

[0005] At present, the commonly used methods for detecting total homocysteine ​​include high-performance liquid chromatography, enzyme-linked immunosorbent assay, chemiluminescence method, biochemical method, and cyclic enzyme method. In the current international market, the methods that can be used in biochemical analysis instruments There are two kinds of HCY reagents, both of which are cycle enzyme methods, one is direct method (hydrolase cycle enzyme method), and the other is indirect method (cystathionine cycle enzyme method), in which hydrolase cycle enzyme method has high specificity, It has strong anti-interference ability and can eliminate endogenous β-cystathionine. It is suitable for various biochemical instruments. This type of product is widely used by large international companies. Reagents for the indirect method of cystathionine cycle enzyme method are easily interfered by lipase and iron reagents. The advantage of the indirect method is that the price is cheap, but it also needs to be operated on large-scale equipment such as biochemical analyzers, but none of the above methods can meet the needs of rapid and convenient detection in primary hospital inspection sites. Therefore, the present invention proposes a siphon type total homocysteine Acid test paper and its production method to solve the problems in the prior art

Images