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Gel material, preparation method and application thereof

A gel material and gel technology, applied in the field of biomedical materials, can solve the problems of cross-linked hydrogel performance without endogenous polyamine and hyaluronic acid cross-linking reaction conditions, and achieve low elastic modulus loss rate , Improve the effect of thermal stability

Pending Publication Date: 2022-01-07
IMEIK TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are few reports on the use of endogenous polyamines such as spermidine as hyaluronic acid gel cross-linking agents, and there are almost no reports on the cross-linking reaction conditions of endogenous polyamines and hyaluronic acid and the cross-linking of hydrogels. performance report, in view of this, the present invention is proposed

Method used

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  • Gel material, preparation method and application thereof
  • Gel material, preparation method and application thereof
  • Gel material, preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0078] Example 1: Preparation of double-site cross-linked hydrogel of spermine and hyaluronic acid catalyzed by HATU

[0079] Weigh 3.0g of sodium hyaluronate (molecular weight 900KDa, containing 7.4mmol of hyaluronic acid repeating structural units), then add 98mL of purified water, after completely dissolving, the concentration of hyaluronic acid is 30mg / mL at this time, add to hyaluronic acid 0.03 g of spermine (0.15 mmol in moles) was added into the solution, and the content of spermine accounted for 1% of the mass of hyaluronic acid (2% of the moles of repeating structural units of hyaluronic acid). Use 6mol / L hydrochloric acid solution to adjust the pH value of the hyaluronic acid solution to about 6.20, then add and stir evenly, add 0.32mmol HATU, 213% of the molar number of spermine, continue to stir evenly, seal it and put it in a 25°C incubator for reaction 24h. After the reaction was completed, 40 mL of absolute ethanol was added, and the gel particles were pulveri...

Embodiment 2

[0080] Example 2: Preparation of three-site cross-linked hydrogel of spermidine and hyaluronic acid catalyzed by HATU

[0081] Weigh 3.0g of sodium hyaluronate (molecular weight 900KDa, containing 7.4mmol of hyaluronic acid repeating structural units), then add 98mL of purified water, after completely dissolving, the concentration of hyaluronic acid is 30mg / mL at this time, add to hyaluronic acid 0.022 g of spermidine (0.15 mmol in moles) was added to the solution, and the content of spermidine accounted for 0.7% of the mass of hyaluronic acid (2% of the moles of repeating structural units of hyaluronic acid). Add 0.48mmol HATU, which is 320% of the mole number of spermidine, stir evenly, adjust the pH value of the hyaluronic acid solution to about 5.40 with 6mol / L hydrochloric acid solution, then add it and stir well, seal it and put it in a 50°C incubator Reaction 14h. After the reaction was completed, 40 mL of absolute ethanol was added, and the gel particles were pulveriz...

Embodiment 3

[0082] Example 3: Preparation of four-site cross-linked hydrogel of spermine and hyaluronic acid catalyzed by HATU

[0083] Weigh 3.0g of sodium hyaluronate (molecular weight 900KDa, containing 7.4mmol of hyaluronic acid repeating structural units), then add 98mL of purified water, after completely dissolving, the concentration of hyaluronic acid is 30mg / mL at this time, add to hyaluronic acid 0.03 g of spermine (0.15 mmol in moles) was added into the solution, and the content of spermine accounted for 1% of the mass of hyaluronic acid (2% of the moles of repeating structural units of hyaluronic acid). Add 0.65mmol HATU, which is 433% of the molar number of spermine, stir well, adjust the pH value of the hyaluronic acid solution to about 5.30 with 6mol / L hydrochloric acid solution, continue stirring evenly, seal it and put it in a 60°C thermostat for 14h. After the reaction was completed, 40 mL of acetone was added, and the gel particles were pulverized with an IKAT25 high-she...

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Abstract

The invention provides a gel material, a preparation method and application thereof. The gel material is obtained by crosslinking endogenous polyamine and hyaluronic acid, wherein the endogenous polyamine comprises spermine and / or spermidine, and the cross-linking of the endogenous polyamine and the hyaluronic acid comprises double-site cross-linking, three-site cross-linking or four-site cross-linking. According to the invention, various properties of the gel and the degradation and release speed of polyamine are influenced by controlling reaction crosslinking sites; and after the gel is subjected to moist heat sterilization, the elastic modulus loss rate is low, the rheological property of the gel before sterilization can be effectively maintained, the thermal stability of the hyaluronic acid gel is improved to a great extent, and the usability of the gel in the fields of soft tissue filling, soft tissue repair, medical cosmetology and the like is also improved.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a gel material and its preparation method and application. Background technique [0002] Hyaluronic acid or sodium hyaluronate (hyaluronic acid, referred to as HA), also known as hyaluronic acid, is composed of D-glucuronic acid through β-1,4 glycosidic bonds and N-acetylglucosamine through β-1,3 glycosidic bonds Composed of disaccharide units glycosaminoglycans. It is widely used in the field of cosmetics or eye surgery, and can also be used as a soft tissue filler to repair wrinkles and some soft tissue defects. Hyaluronic acid is the original substance in the body, has good biocompatibility and has certain biological activity, but exogenous hyaluronic acid will be degraded by hyaluronidase in the body, resulting in shortened residence time in the body, resulting in treatment The effect is shortened and multiple injections are required to achieve the therapeutic ...

Claims

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Application Information

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IPC IPC(8): C08J3/24C08J3/075C08L5/08C08K5/17C08B37/08A61L27/52A61L27/20A61K9/06A61K47/36
CPCC08J3/24C08J3/075C08B37/0072A61L27/52A61L27/20A61K9/06A61K47/36C08J2305/08C08K5/17C08L5/08
Inventor 李睿智谷诗伟张堃
Owner IMEIK TECH DEV CO LTD