Preparation method of electrochemical luminescence aptamer sensor for detecting lead in water
An aptamer sensor, electrochemical technology, applied in the direction of chemiluminescence/bioluminescence, electrochemical variables of materials, scientific instruments, etc., can solve the problems of complex sample processing, limited wide application, wide detection range, etc.
Pending Publication Date: 2022-01-07
SHANDONG UNIV OF TECH
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[0003] Heavy metal detection methods: At present, heavy metal detection methods mainly include gas chromatography, high performance liquid chromatography, atomic absorption spectrometry, spectrophotometry, atomic emission spectrometry, inductively coupled plasma mass spectrometry, colorimetry and other traditional methods. Although heavy metal detection methods are relatively mature in application, high in sensitivity and accuracy, low in detection limit, and wide in detection range, etc., the instruments are expensive, time-consuming, complicated in sample processing, inconvenient to carry, unable to continuously monitor and measure on-site, etc. limit its wide application
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[0025] (1) Preparation of MXene@Au: 12 mL of 0.5 mg / mL MXene was ultrasonically dispersed for 10 min, and then 60 μL of 1% chloroauric acid (HAuCl 4 . 3H 2 O), after reacting for 30 min, add 50 μL of 0.16 mol / L sodium borohydride (NaBH 4 ) and 1.6 mol / L sodium hydroxide (NaOH) (volume ratio of the two = 1:1), sonicate for 30 min, centrifuge at 10,000 rpm for 8 min with a centrifuge, and wash with ultrapure water for 3 times , vacuum-dried at 60°C, and prepared as a 5 mg / mL solution.
[0026] (2) Preparation of bipyridyl ruthenium: Dissolve chitosan in 1% acetic acid solution to prepare a 0.05% chitosan solution, add bipyridyl ruthenium to prepare a 1 mM solution, sonicate for 10 min, and mix well .
[0027] (3) Preparation of nano-gold solution: soak all glass containers that may be used in the experiment in aqua regia (volume ratio of nitric acid to hydrochloric acid = 1:3) for 24 h, wash with ultrapure water 5 times, and dry until use. Measure 200 mL of ultrapure water, ...
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The invention discloses preparation and use methods of an electrochemical luminescence aptamer sensor for detecting lead ions in water, which are characterized in that a switch type electrochemical luminescence biosensor for detecting lead ions in water is constructed by utilizing the conductivity and the high specific surface area of a titanium carbide and gold compound, the pi-pi conjugate strong adsorption effect of bipyridyl ruthenium, the strong catalytic effect of a gold and silicon dioxide compound and the shearing recognition effect of the lead ions on an aptamer. The sensor can judge whether detected water contains target ions or not according to the change of the electrochemiluminescence peak value of the lead ions in the detection result. The sensor has the advantages that the sensor is low in cost and simple in detection operation; the lead heavy metal can be qualitatively and quantitatively determined; and the sensor has high sensitivity and low detection limit, and provides a thought for detection of other ions and small molecular substances.
Description
technical field [0001] The invention relates to an electrochemiluminescence aptasensor, a preparation method and a use method of the sensor, and belongs to the technical field of electrochemiluminescence aptasensor. Background technique [0002] Water in nature is an indispensable condition for biological survival. With the acceleration of my country's industrialization process and the improvement of agricultural production technology, water pollution is becoming more and more serious; heavy metal pollution in water comes from a wide range of sources, mainly from industrial pollution, agricultural pollution and domestic pollution. Increasing water pollution has posed a major threat to human survival and has become a major obstacle to human health, economic and social sustainable development. Heavy metal pollution to the water environment not only harms animals and plants, but also directly affects the quality of drinking water for human life and directly or indirectly threat...
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IPC IPC(8): G01N27/327G01N27/49G01N21/76
CPCG01N27/3277G01N27/49G01N21/76
Inventor 张妍妍翟鸿国王悦孙霞郭业民
Owner SHANDONG UNIV OF TECH