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Application of recombinant human I-type collagen in preparation of material for promoting wound healing

A technology of collagen and healing materials, applied in the field of bioengineering, can solve the problems of complex properties and hidden dangers of animal viruses that cannot be completely eliminated, and achieve the effects of short onset time, outstanding cell repair ability, and fast wound healing

Pending Publication Date: 2022-01-11
JIANGSU JLAND BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, natural animal collagen has different molecular weights and complex properties, and the hidden dangers of animal viruses cannot be completely eliminated during the application process.

Method used

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  • Application of recombinant human I-type collagen in preparation of material for promoting wound healing
  • Application of recombinant human I-type collagen in preparation of material for promoting wound healing
  • Application of recombinant human I-type collagen in preparation of material for promoting wound healing

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Protein sequence selection

[0028] The amino acid sequence (SEQ No.1) of human type I α1 chain collagen was analyzed for hydrophobicity, and the evaluation results were as follows: figure 1 shown. The lower the hydrophobicity evaluation score, the better the hydrophilicity. According to the results of hydrophobicity analysis, amino acid fragments with low scores are selected, and these fragments are integrated into a new protein, that is, the recombinant human type I collagen of the present invention (SEQ No. 2). Hydrophobic analysis of the amino acids of recombinant human type Ⅰ collagen, the results are as follows figure 2 As shown, the hydrophobicity evaluation of all amino acids in this protein is lower than zero, indicating that the protein is very hydrophilic.

[0029] 2. Plasmid construction and linearization

[0030] The recombinant human type Ⅰ collagen was translated into base sequence (SEQ No.3), the gene Iα1 510aa was synthesized by PAS (PCR-basedAc...

Embodiment 2

[0063] In vitro cell scratch test:

[0064] 1. Plate human keratinocytes on a 12-well plate, add DMEM medium containing 10% fetal bovine serum, place in 5% CO 2 , Cultivate in a 37°C incubator until the cells adhere to the wall and completely cover the culture plate to form a monolayer of cells.

[0065] 2. Use a 10 μL sterile pipette tip to make a "one" scratch on the monolayer of cells to form an artificial wound, and wash with PBS 3 times.

[0066] 3. Dosing and incubation, set blank control group, 0.1% (w / v, g / mL, ie 1g / L) tilapia skin collagen group and 0.1% recombinant human type Ⅰ collagen group.

[0067] 4. Take pictures of the cell culture at 0h, 2h, 8h, and 24h respectively, and take three wound edge data from each well for statistical analysis.

[0068] Table 2 The width between wound edges in each group at different times

[0069]

[0070]

[0071] Table 3 Different detection time, the reduction percentage of artificial wound edge width in each group

[0...

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Abstract

The invention discloses an application of recombinant human-derived I-type collagen in preparation of a material for promoting wound healing. The amino acid sequence of the recombinant human I-type collagen is shown as SEQ No.2, which contains 510 amino acids, the theoretical molecular weight is about 55.911 kd, and the constructed recombinant human I-type collagen expression strain can effectively, stably and massively express the recombinant human I-type collagen. The recombinant human collagen has good hydrophilicity and stability, the structure of the recombinant human collagen is 100% the same as that of a corresponding part of a natural collagen gene sequence, immunological rejection is not caused when the recombinant human collagen is applied to a human body, the recombinant human collagen has the advantages of short onset time and high wound healing speed when being used as a wound healing promoting material, and the recombinant human I-type collagen is suitable for the fields of biomedical materials and cosmetics.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and relates to the application of recombinant human type I collagen ((RHCI)) in the preparation of materials for promoting wound healing. Background technique [0002] Wound repair and healing is a complex and dynamic biological process. Healing includes four steps: hemostasis, inflammatory response, granulation tissue regeneration, and matrix remodeling. The smooth progress of the healing process is an important prerequisite to ensure skin repair and functional recovery, and the role of granulation tissue and epithelial regeneration in wound healing is crucial. [0003] Collagen is the most important structural protein in vertebrates, accounting for about 25-30% of the total protein. As the most abundant protein in the extracellular matrix, collagen is the most important structural protein in connective tissue and almost all parenchyma interstitial tissues, and plays a role in stabilizin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/39A61K8/65A61L15/32A61L15/44A61P17/02A61Q19/00C07K14/78C12N15/12C12N15/81
CPCA61K38/39A61K8/65A61Q19/00A61P17/02A61L15/325A61L15/44C07K14/78C12N15/815A61L2300/412A61L2300/252
Inventor 赵健烽朱逸丽余继刚高力虎冯丽萍黄建民
Owner JIANGSU JLAND BIOTECH CO LTD
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