Preparation method and application of novel electrochemical sensor for detecting chlorogenic acid

An electrochemical and sensor technology, which is used in the detection of agricultural products, cosmetics, food, nano-functional materials and electrochemical sensing to achieve high sensitivity, good electrocatalysis, and low detection limit.

Pending Publication Date: 2022-01-18
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, there is no ZIF-8@Ag / MWCNTs combination method and a method for successfully and efficiently preparing ZIF-8@Ag / MWCNTs composite materials, and the MOFs composite modified electrode is applied to chlorogenic acid and samples containing chlorogenic acid also not reported

Method used

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  • Preparation method and application of novel electrochemical sensor for detecting chlorogenic acid
  • Preparation method and application of novel electrochemical sensor for detecting chlorogenic acid
  • Preparation method and application of novel electrochemical sensor for detecting chlorogenic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: the preparation of electrochemical sensor

[0025] (1) Put 30–50 mg of multi-walled carbon nanotubes in 60 mL of mixed acid (concentrated nitric acid:concentrated sulfuric acid=1:(1–3)), continuously sonicate for 8–10 h to carboxylate the carbon nanotubes, and then use The product mixture was washed repeatedly until neutral by a suction filtration device, and dried in a vacuum oven for 24 h to obtain carboxylated multi-walled carbon nanotubes, which were set aside.

[0026] (2) ZIF-8@Ag was prepared by the following method: 200-300 mg of high-purity zinc acetate and 1-8 g of high-purity 2-methylimidazole were placed in 60-100 mL of ethanol and stirred evenly, keeping While stirring, pour the zinc acetate solution into the 2-methylimidazole solution, stop stirring after 0.5-1 h, and let the emulsion stand at room temperature for 24 h. The ZIF-8 crystals in the emulsion were separated by centrifugation, washed several times with ethanol, and dried in a vacu...

Embodiment 2

[0029] Embodiment 2: Comparison of Modified Electrodes

[0030] Such as image 3 shown in K 3 [Fe(CN) 6 ] / K 4 [Fe(CN) 6] system as a probe, the cyclic voltammetry behavior (CV) of the bare glassy carbon electrode and each modified electrode was tested. The sensor prepared in Example 1 was relatively bare GCE, ZIF-8 / GCE, and MWCNTs modified glassy The cyclic voltammetry response of -8@Ag / MWCNTs / GCE presents the highest current signal. Such as Figure 4 As shown, the chlorogenic acid buffer was scanned with GCE and ZIF-8@Ag / MWCNTs / GCE respectively, and it was found that compared with the bare electrode, the current in the cyclic voltammetry curve of ZIF-8@Ag / MWCNTs / GCE The signal is significantly enhanced.

Embodiment 3

[0031] Embodiment 3: the detection of chlorogenic acid

[0032] The chlorogenic acid stock solution was prepared by dissolving the chlorogenic acid standard in ethanol, and the chlorogenic acid solutions with different concentrations were obtained by gradient dilution using PBS buffer solution with pH=6 for electrochemical detection. Connect the three-electrode system to an electrochemical workstation, and use differential pulse voltammetry to determine chlorogenic acid, such as Figure 5 As shown, the oxidation peak current intensity has a linear relationship with the concentration of chlorogenic acid in the following range: 0.05 μmol~10 μmol / L. The linear relationship is:

[0033]

[0034] Such as Figure 6 As shown, the new modified electrode has a good linear relationship to chlorogenic acid, R 2 =0.99812, and has a wider linear range and a lower detection limit of 0.0138 μmol.

[0035] Example 3: Anti-interference test of ZIF-8@Ag / MWCNTs / GCE

[0036] Taking the ±5...

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Abstract

The invention provides a preparation method of an electrochemical sensor based on an MWCNTs/ZIF-8 (at) Ag composite material, and is applied to detection of a phenolic antioxidant chlorogenic acid. A carboxylated carbon nanotube and a ZIF-8 (at) Ag composite material are respectively prepared, the carboxylated carbon nanotube is fixed on a glassy carbon electrode by chitosan to serve as a substrate material, ZIF-8 (at) Ag is superposed and fixed on the substrate material to obtain a modified working electrode, and the electrochemical sensor prepared according to the method can be accurately, simply, conveniently and quickly used for sensitive detection of chlorogenic acid.

Description

technical field [0001] The invention relates to the technical field of nano functional materials and electrochemical sensing, and belongs to the detection technical field of food, agricultural products, cosmetics and other fields. Background technique [0002] Chlorogenic acid (CGA) is a widely distributed antioxidant substance with a significant phenolic structure. It is a combination of caffeic acid and quinic acid and is distributed in various fruits and vegetables. Chlorogenic acid has antioxidant, anticancer, anti-inflammatory and antiviral activities, so it has been widely used in health care, medical and food processing industries. Due to the important functional properties of chlorogenic acid, the detection and analysis of chlorogenic acid has attracted more and more attention in recent years. At present, the common detection methods for chlorogenic acid include colorimetric method, high performance liquid chromatography, combined method, fluorescent sensor method, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30
CPCG01N27/26G01N27/30
Inventor 周泉城盛桂华董雷超王红磊南希骏嵇威厉佳怡
Owner SHANDONG UNIV OF TECH
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