Dry glucose test paper strip adopting Prussian blue nano-enzyme and preparation method thereof

A Prussian blue and nanozyme technology, applied in the field of clinical diagnosis, can solve the problems of high price, poor thermal stability, harsh preparation conditions, etc., and achieve the effects of convenient synthesis, good thermal stability, and improved competitiveness.

Pending Publication Date: 2022-02-01
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Purpose of the invention: the technical problem to be solved in the present invention is to provide a kind of dry glucose test strip that adopts Prussian blue nanoparticles to replace natural horseradish peroxidase, to solve the problem of using horseradish peroxidase in the prior art. Glucose test strips have poor thermal stability, high price, and harsh preparation conditions

Method used

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  • Dry glucose test paper strip adopting Prussian blue nano-enzyme and preparation method thereof
  • Dry glucose test paper strip adopting Prussian blue nano-enzyme and preparation method thereof
  • Dry glucose test paper strip adopting Prussian blue nano-enzyme and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0052] Preparation method of glucose test paper using Prussian blue artificial enzyme. In this example, Prussian blue nanoparticles, glucose oxidase and substrate are on the same reaction membrane; see figure 1

[0053] (1) Configure membrane treatment solution (1L) containing Prussian blue nanozyme:

[0054]

[0055] (2) Treat the reaction membrane with a membrane treatment solution, and fix the Prussian blue nanoparticles and other necessary reactants on the membrane; the way of soaking or marking can be adopted. The parameters for scribing are preferably 5.5 μL / cm, and the scribing speed: 50 mm / s.

[0056] (3) Place the film treated with the above substances on a rack of appropriate size, and dry it at 37° C. for 1 hour.

[0057] (4) Cut the dried reaction membrane into a suitable size, and assemble it with the blood filter membrane and the diffusion membrane to form a test strip.

Embodiment 2

[0059] Preparation method of glucose test paper using Prussian blue artificial enzyme. The materials and reagents used in this example are the same as those in Comparative Document 1. The difference is that the Prussian blue nanoparticles in this example are adsorbed on the filter membrane alone, and the glucose oxidase and the substrate are on an independent reaction membrane; see figure 2 . The filter membrane containing Prussian blue nanoparticles can be prepared and stored separately, has good stability, and can be used as a filter membrane with peroxide activity in any biochemical index that requires peroxide catalysis.

Embodiment 3

[0061] Electron microscope characterization of Prussian blue artificial enzyme test paper reaction film in embodiment 1. Figure 3-6 A blank asymmetric polyethersulfone membrane ( image 3 , Figure 4 ) and scanning electron microscope images of Prussian blue nanoparticles ( Figure 5 , Figure 6 ). It can be seen from the figure that the particle size of Prussian blue nanoparticles is around 110nm, the particle size distribution is uniform, and it is a complete cubic structure with a perfect shape, which is the basis of its nanozyme activity. The polyethersulfone membrane fiber cords are clear, and the pore size distribution is gradiently distributed from 200um in diameter on one side to 10um in the other side. This asymmetric structure facilitates the liquid sample from the upper layer with large aperture to infiltrate the membrane material, while the surface of the lower layer with small aperture is very dense and flat, which is conducive to the collection of light refl...

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Abstract

The invention discloses a dry glucose test paper strip adopting Prussian blue nano-enzyme and a preparation method thereof, and belongs to the technical field of clinical diagnosis. According to the invention, Prussian blue nano-enzyme is adopted to replace natural horse radish peroxidase commonly used in a dry chemical test paper strip; the artificially synthesized Prussian blue nano material has the characteristic of catalyzing the reaction of peroxide and a substrate; and compared with a natural enzyme, the artificial enzyme has the advantages of being easy to store, convenient to prepare, low in cost, good in thermal stability and the like, can be industrially produced on a large scale, and can meet the requirements of clinical application.

Description

technical field [0001] The invention relates to a dry glucose test strip using Prussian blue nanozyme, which belongs to the technical field of clinical diagnosis. Background technique [0002] Most routine clinical biochemical substances will produce hydrogen peroxide after a series of enzyme-catalyzed reactions, so that they can undergo a color reaction with the substrate under the action of peroxidase, and complete quantitative testing for the corresponding concentration indicators. In the emergency department or bedside diagnosis, there are high requirements for the sampling volume of the sample, the simplicity of the analysis process and the reporting time of the test results. The dry chemical method, in which substances involved in biochemical reactions are dried on layers of membranes, can satisfy fingertip blood testing. It is easy to operate, and the result can be obtained within 3 minutes. It is suitable for emergency department and bedside diagnosis. [0003] Comp...

Claims

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Application Information

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IPC IPC(8): C12Q1/54C12Q1/26
CPCC12Q1/54C12Q1/26C12Q2326/96
Inventor 童鎏顾宁苏恩本张宇陆颖琪宰云峰黄力
Owner SOUTHEAST UNIV
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