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Application of fermentation liquor of alternaria alternata JTF001 in inhibition of seed germination of Orobanche aegyptiaca

A technology of JTF001 and Alternaria, which is applied in the field of inhibiting the germination of gualiedang seeds, can solve the problems of unclear function and excessive fermentation liquid, and achieve a significant inhibitory effect

Active Publication Date: 2022-02-08
INST OF PLANT PROTECTION OF XINJIANG ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Our previous research found that the crude extract of Alternaria alternata can prevent and control the germination of gualistang seeds, but there are many components in its fermentation broth, and it is not clear which components play a role

Method used

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  • Application of fermentation liquor of alternaria alternata JTF001 in inhibition of seed germination of Orobanche aegyptiaca
  • Application of fermentation liquor of alternaria alternata JTF001 in inhibition of seed germination of Orobanche aegyptiaca
  • Application of fermentation liquor of alternaria alternata JTF001 in inhibition of seed germination of Orobanche aegyptiaca

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Experimental program
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Effect test

Embodiment 1

[0038] The extraction method of embodiment 1 active substance

[0039] (1) Solid fermentation of Alternaria alternata JTF001

[0040] The preserved Alternaria strain was transferred to PDA medium and cultured at 28°C for 5 days. Take the fungus cake and add it into a semi-solid seed solution in a triangular flask containing a semi-solid medium (recipe: peptone 10g, maltose 40g, yeast extract powder 10g, agar 4g and distilled water 1000mL), and heat it at 28°C, 180r min -1 Under shaking culture for 48h. Add 5 mL of semi-solid seed liquid to rice solid medium (recipe: put 80 g of rice, 1.2 g of yeast powder and 120 mL of distilled water in a 500 mL Erlenmeyer flask, soak overnight and then sterilize at 121 °C for 30 min), and incubate at 28 °C for 15 days .

[0041] (2) Extraction of crude extract

[0042] Mechanically stir the rice solid culture medium, add 240mL ethyl acetate to each bottle, soak for 24h, sonicate for 1h, and extract twice. After the ethyl acetate soaking...

Embodiment 2

[0051] Example 2 Structure Identification of Compounds LF6-2 and LF12-29-3

[0052] Compound LF6-2: Pale yellow oily solid, easily soluble in methanol and deuterated acetone. The [M-H] of the compound according to the mass spectrum - The molecular ion peak is 197.00 ( Figure 5 ), and its molecular formula is inferred to be C 10 h 15 NO 3 , further through its H NMR spectrum ( 1 H-NMR) and carbon spectrum ( 13 C-NMR) and the known compound Tenuazonic acid reported in the literature (T Rosett.1957,67:390-400) are compared and analyzed, and its structural formula is determined as shown in formula I, and its chemical name is 3-acetyl-5-second-butyl Base-4-hydroxy-3-pyrrolin-2-ol.

[0053]

[0054] Compound LF12-29-3: Pale yellow oily solid, easily soluble in methanol and deuterated acetone. The [M+H]+molecular ion of compound according to mass spectrometry is divided into 292.00 ( Figure 10 ), and its molecular formula is inferred to be C 15 h 16 o 6 , and further...

Embodiment 3

[0063] Example 3 Determination of the Inhibitory Effect of Natural Compounds LF6-2 and LF12-29-3 on Gualidang Seed Germination

[0064] a. Sterilization of melon seeds: sterilize melon seeds in 75% alcohol for 1.5 minutes, then transfer to 1% sodium hypochlorite for 12 minutes, and dry them on sterile filter paper for later use.

[0065] b. Solution preparation: The solution in the NMR tube was blown with nitrogen, and after drying, the measured masses of LF6-2 and LF12-29-3 were 20.00 mg and 7.00 mg, respectively. Add 100 μL of methanol to dissolve, then add sterile water to make 8mM mother solution, and then dilute it to 4mM, 2mM and 1mM.

[0066] c. Determination of germination inhibition of gualiedang seeds: add about 40 sterilized and dried gualiedang seeds to each well of a 24-well plate, add 0.5mL strigolactone (GR24) and 0.5mL of 8mM and 4mM respectively to the treatment group , 2mM and 1mM compound solutions, the final concentrations of LF6-2 and LF12-29-3 compounds ...

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Abstract

The invention provides application of fermentation liquor of alternaria alternata JTF001 in inhibition of seed germination of Orobanche aegyptiaca. The fermentation liquor of the alternaria alternata JTF001 comprises 3-acetyl-5-sec-butyl-4-hydroxy-3-pyrrolin-2-one, and / or 2,3,4,5'-tetrahydro-2,3,7-trihydroxy-9-methoxy-4'-methyl-dibenzo-6-pyrone. The invention discloses the effect of 3-acetyl-5-sec-butyl-4-hydroxy-3-pyrrolin-2-one and 2,3,4,5'-tetrahydro-2,3,7-trihydroxy-9-methoxy-4'-methyl-dibenzo-6-pyrone in fermentation liquor of alternaria alternata JTF001 on inhibition of seed germination of Orobanche aegyptiaca for the first time, and the two compounds can be directly used for preventing and treating Orobanche aegyptiaca, and the inhibiting effect on Orobanche aegyptiaca is obvious.

Description

technical field [0001] The invention relates to the technical field of applied agricultural microorganisms and biological control, in particular to the application of the fermented liquid of Alternaria alternata JTF001 in inhibiting the germination of gualiedang seeds. Background technique [0002] Guaretang (Orabanche aegyptaica) is a whole parasitic higher plant, which is widely distributed in the Mediterranean region, western Asia and eastern Europe. Grass. Gualiedang in my country is mainly distributed in Xinjiang, Jilin, Gansu, Heilongjiang, Hebei, Shandong, Shanxi, Shaanxi, Liaoning, Qinghai, Inner Mongolia, Sichuan and other provinces. my country's Xinjiang is an area where the distribution and damage of melons are serious, and it can cause more than half of the production reduction of melons, watermelons and tomatoes. Due to the parasitic nature of Gualedang root, its harm to the host mainly occurs in the underground growth period before emergence, so the common we...

Claims

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Application Information

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IPC IPC(8): A01N63/30A01N43/36A01N43/16A01P13/00C07D207/38C07D311/80
CPCA01N63/30A01N43/36A01N43/16C07D207/38C07D311/80
Inventor 罗文芳许建军何伟李彦谢丙炎周军辉孔繁阳
Owner INST OF PLANT PROTECTION OF XINJIANG ACADEMY OF AGRI SCI
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