Deep eutectic solvent for degrading algal polysaccharides as well as preparation method and application of deep eutectic solvent

Abstract

The embodiment of the invention discloses a deep eutectic solvent for degrading algal polysaccharides as well as a preparation method and application of the deep eutectic solvent, and belongs to the technical field of deep processing of algal polysaccharides. The deep eutectic solvent is formed by mixing a hydrogen bond acceptor and a hydrogen bond donor, and the dosage of the hydrogen bond acceptor is 30-40% of the mass of the eutecticevaporate solvent; wherein the hydrogen bond acceptor is choline chloride, and the hydrogen bond donor is composed of glucose, urea and citric acid in a mass ratio of (24-30): (25-35): (5-11). The deep eutectic solvent has the advantages of natural source, safety, no toxicity, biodegradability and the like, meets the industrial development direction and the environmental protection requirement, and meanwhile, when the deep eutectic solvent is used for degrading the algal polysaccharides, the molecular weight of algal polysaccharide degradation products is controllable by controlling degradation conditions, and a novel method is provided for preparation of algal polysaccharide degradation products with specific molecular weight.

Description

technical field [0001] The embodiment of the present invention relates to the technical field of deep processing of seaweed polysaccharides, in particular to a deep eutectic solvent for degrading seaweed polysaccharides, its preparation method and application. Background technique [0002] Seaweed is rich in polysaccharides, including alginic acid, agar, carrageenan, fucoidan (also known as fucoidan), Laminaran (containing β-1.3-glucan structure), Ulvans (ulva polysaccharide, rich in sulfated mouse Li sugar), Porphyran (seaweed polysaccharide), etc. Seaweed polysaccharides that have been industrially produced on a large scale include sodium alginate (structure is polyuronic acid sodium salt), agar (structure is galactose-anhydrogalactose polymer), karaage Gum (structure is polygalactose sulfate), fucoidan (structure is sulfated fucoidan), and other seaweed polysaccharides are sold as research samples but have not yet been industrialized. At present, seaweed polysaccharides ...

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Problems solved by technology

However, the above methods all need to dissolve the polysaccharide in water (water phase) and then degrade it. For example, the concentration of seaweed polysaccharide in the water phase in CN112920289A is 2.5~10mg / mL, which is only 0.25%~1% by mass percentage; Junjun Yan et al. (Int J Biol Macromol.2020 Oct 1;160:288-295) When preparing new agarobiose by enzymatic hydrolysis of agar, the concentration of agar in aqueous solution is only 0.5%~1.0%, w / v; for industrial production, this Substrate concentration is not ideal

The reason for the low concentration of polysaccharide in aqueous solution is that high-concentration polysaccharide aqueous solution has high viscosity, and it is difficult to carry out process operations such as stirring and pumping of high-viscosity solution, so it can only be configured as a low-concentration polysaccharide aqueous solution, resulting in system efficiency. lower

In addition, after the polysaccharide is degraded in the water phase, a large amount of water needs to be removed to separate the degradation product from the water phase. The removal of water generally uses evaporation or membrane treatment, which has high energy consumption, long time consumption, and attenuation of the flux of the membrane element. And other issues

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