Preparation method of surface-energy-controllable aluminum hydroxide nano adjuvant

A kind of aluminum oxyhydroxide and nanotechnology, which is applied in the field of preparation of aluminum oxyhydroxide materials, can solve the problems of lack of systematic research on the adjuvant effect of surface properties, etc., and achieve the effect of low raw material price, easy operation and simple process

Pending Publication Date: 2022-02-11
DALIAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, for the AlOOH adjuvant, the regulation of its specific surface properties and its influence on the adjuvant effect are still lacking in systematic exploration.

Method used

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  • Preparation method of surface-energy-controllable aluminum hydroxide nano adjuvant
  • Preparation method of surface-energy-controllable aluminum hydroxide nano adjuvant
  • Preparation method of surface-energy-controllable aluminum hydroxide nano adjuvant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] This example provides a method for preparing an AlOOH nano-adjuvant with controllable surface free energy. The specific steps are as follows:

[0040] Weigh 4.1799g of aluminum nitrate nonahydrate and dissolve it in deionized water at room temperature to prepare a clear and transparent aluminum nitrate solution with an aluminum concentration of 70 mg / mL, which is continuously stirred at a speed of 400 rpm. At room temperature, slowly add ethylenediamine dropwise into the aluminum nitrate solution (dropping rate 20 μL / min), and it can be observed that a white jelly is produced during the process. During this process, a pH meter is used to monitor the pH of the reaction system in real time. When it is observed that the pH values ​​of the reaction system reach the specified values ​​(4, 5, 6), stop adding ethylenediamine dropwise and continue stirring for 15 minutes to ensure that the reaction system is mixed evenly and the pH is stable. Afterwards, the mixtures with diffe...

Embodiment 2

[0045] This embodiment provides a mouse intramuscular injection model and implementation method for evaluating the immune effect of engineering AlOOH nano adjuvants. The specific implementation steps are as follows:

[0046] Prepare the vaccine preparation under aseptic conditions, the AlOOH nano adjuvant obtained in Example 1 and the hepatitis B surface antigen (HBsAg) are subjected to equal volume oscillation adsorption, and the final vaccine preparation concentration is preferably 1 mg / mLAl, 40 μg / mL HBsAg. After the adjuvant antigen was fully adsorbed, the mice were immunized intramuscularly with a volume of 50 μL each, and boosted with the same method and dose on the 21st day. 42 days after the initial immunization, the mice were killed, the mouse serum was separated, and the antibody titers (including total IgG, IgG 1 ) levels are measured, such as Figure 5 . Among them, the mice in the ctrl group were only injected with an equal volume of normal saline, and the mice ...

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Abstract

The invention relates to the field of biological medicine, and particularly discloses a preparation method of a surface-energy-controllable aluminum hydroxide nano adjuvant. The method is based on a hydrothermal synthesis method, the specific orientation of crystal growth is controlled by changing the pH value of a precipitation system, and finally the purpose of controlling the surface free energy of the AlOOH nano material is achieved. The method is simple to operate, raw materials are low in price, and reaction products are good in stability and repeatability. The prepared hydroxyl alumina nano material is uniform in morphology and dispersion, and has a good prospect in vaccine preparation and production. Besides, the vaccine preparation formula based on the AlOOH adjuvant and the immune effect verification method provided by the invention are simple and high in operability, and a good strategy is provided for engineering development and further clinical transformation of the vaccine preparation.

Description

technical field [0001] The invention relates to a preparation method of an aluminum oxyhydroxide material, in particular to an aluminum oxyhydroxide (AlOOH) nano adjuvant with controllable surface energy, a preparation method thereof and biological effects thereof. Background technique [0002] The emergence of vaccines has greatly curbed the invasion of infectious diseases on human society. Among them, as a main component of vaccines, adjuvants play an important role in reducing the dosage of antigens, reducing the number of vaccinations, improving the immunogenicity of antigens and improving the immune efficacy of vaccines. Among them, aluminum oxyhydroxide adjuvant is still the most widely used inorganic adjuvant due to its good biological safety and the ability to effectively enhance the immune response. However, there are still many vacancies in the research on aluminum salt adjuvant, its physical and chemical properties are not clear, and the specific mechanism in the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/00A61K39/215A61K39/29A61P31/14C01F7/34C01F7/36
CPCA61K39/39A61K39/00A61K39/12A61P31/14A61P31/20A61P1/16C01F7/34C01F7/36A61K2039/5258A61K2039/5252A61K2039/54A61K2039/55505C12N2770/20034C12N2730/10134C01P2004/10C01P2006/22A61K2300/00
Inventor 孙冰冰梁智慧王馨
Owner DALIAN UNIV OF TECH
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