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Double-layer artificial periosteum, preparation method and application thereof

An artificial bone, double-layer technology, applied in the fields of medical science, prosthesis, tissue regeneration, etc., can solve the problem that the periosteum cannot effectively inhibit osteosarcoma bone cancer, etc., and achieve the effect of preventing the growth into the bone defect area

Active Publication Date: 2022-02-11
ASIA BIOMATERIALS WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of this application is to provide a double-layer artificial periosteum and its preparation method and application to solve the problem that the existing periosteum cannot effectively inhibit osteosarcoma and treat bone cancer

Method used

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  • Double-layer artificial periosteum, preparation method and application thereof
  • Double-layer artificial periosteum, preparation method and application thereof
  • Double-layer artificial periosteum, preparation method and application thereof

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preparation example Construction

[0048] According to a typical implementation of the present invention, a method for preparing a double-layer artificial periosteum is provided, the method comprising:

[0049] S1, the preparation of dense layer, specifically include:

[0050] S1-1. Dissolving silk fibroin in lithium bromide solution or calcium chloride ternary system (calcium chloride / water / ethanol molar ratio 1:8:2) solution to obtain silk fibroin with a mass fraction of 5% to 20% protein solution.

[0051] The silk fibroin is selected from the natural high-molecular fiber protein extracted from silkworm cocoons or silk, and the mass fraction of silk fibroin in the silk fibroin solution is 5% to 20%. The reason: too high mass fraction of silk fibroin is not conducive to Degradation is slow during dissolution and use, and the low mass fraction affects the mechanical properties and physical barrier function of the membrane;

[0052] S1-2, taking the silk fibroin solution obtained in step S1-1 and injecting it...

Embodiment 1

[0094] A preparation method of double-layer artificial periosteum, the method comprising:

[0095] S1, the preparation of dense layer, specifically include:

[0096] S1-1. Dissolving silk fibroin in lithium bromide solution or calcium chloride ternary system (calcium chloride / water / ethanol molar ratio 1:8:2) solution to obtain a silk fibroin solution with a mass fraction of 5%.

[0097] S1-2. Take the silk fibroin solution obtained in step S1-1 and inject it into the mold, pre-freeze at -60°C for 12 hours, then dry the frozen body at a temperature of 10°C and a pressure of 10Pa for 48 hours, and then mold it under a pressure of 20MPa 60s to obtain dense layer film;

[0098] Thermally cross-linking the dense layer film at 110° C. for 24 hours in a vacuum oven to obtain a dense layer;

[0099] S2, preparation of selenium-doped hydroxyapatite collagen solution, specifically including:

[0100] S2-1. Dissolving type I collagen in an acetic acid solution with a mass fraction of ...

Embodiment 2

[0111] A preparation method of double-layer artificial periosteum, the method comprising:

[0112] S1, the preparation of dense layer, specifically include:

[0113] S1-1. Dissolving silk fibroin in lithium bromide solution or calcium chloride ternary system (calcium chloride / water / ethanol molar ratio 1:8:2) solution to obtain a silk fibroin solution with a mass fraction of 5%.

[0114] S1-2. Take the silk fibroin solution obtained in step S1-1 and inject it into the mold, pre-freeze at -60°C for 12 hours, then dry the frozen body at a temperature of 10°C and a pressure of 10Pa for 48 hours, and then mold it under a pressure of 20MPa 30s to obtain dense layer film;

[0115] Thermally cross-linking the dense layer film at 110° C. for 24 hours in a vacuum oven to obtain a dense layer;

[0116] S2, preparation of selenium-doped hydroxyapatite collagen solution, specifically including:

[0117] S2-1. Dissolving type I collagen in an acetic acid solution with a mass fraction of ...

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Abstract

The invention particularly relates to a double-layer artificial periosteum, a preparation method and application thereof, and belongs to the technical field of biomedical materials. The double-layer artificial periosteum is characterized in that a compact layer is prepared firstly, and then a loose layer is prepared on the compact layer. According to the invention, an artificial periosteum with a double-layer structure is constructed, wherein a compact layer and a loose layer are synergistically combined, the surface of the compact layer is smooth, the compact layer faces soft tissue during use, the physical barrier function of the periosteum can be well utilized, connective tissue is effectively prevented from growing into a bone defect area, and the regeneration function of specific tissue is played to the maximum extent; the coarse loose layer is consistent with the autogenous bone in component and structure, faces bone tissue during use, can be well attached to a bone defect surface, induces generation of new bones, effectively inhibits osteosarcoma and treats bone cancer; and the obtained double-layer artificial periosteum has good mechanical properties and degradation properties, and also has good capabilities of promoting bone healing, inhibiting osteosarcoma and treating bone cancer.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, in particular to a double-layer artificial periosteum and its preparation method and application. Background technique [0002] Periosteum is a dense connective tissue membrane that covers almost all bone surfaces except joints. It consists of an outer fibrous layer and an inner cambium. It plays an important role in bone development and fracture healing. The fibrous layer contains fibroblasts, collagen and elastic fibers, a network of nerves and microvessels, and provides the mechanical stability of the periosteum. The cambium stores a large number of different types of cells and provides a source of cells for bone formation and repair. Periosteum can provide nutrients to bone tissue through blood vessels, induce bone growth, control the direction of bone growth, etc., and play a vital role in the repair of bone tissue defects. Numerous studies have demonstrated the importance of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/22A61L27/24A61L27/12A61L27/50A61L27/56
CPCA61L27/227A61L27/24A61L27/12A61L27/50A61L27/56A61L2430/02C08L89/00
Inventor 唐三王喆周雄
Owner ASIA BIOMATERIALS WUHAN CO LTD
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