Method for preparing guided tissue regeneration membrane through combination of electrostatic spinning and electrostatic spraying

A technology that guides tissue regeneration and electrostatic spraying. It is used in electrospinning, textiles and papermaking, and pharmaceutical formulations. It can solve unseen problems, achieve good biological activity and biodegradability, regulate cell morphology, and excellent permeability. Effect

Active Publication Date: 2022-03-04
BEIJING ALLGENS MEDICAL SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there are related reports on the use of type I collagen and mineralized collagen nanoparticles to obtain a loose layer through freeze-drying and compounding (for example, see Chinese patent application CN110420359A), but there is no known electrospinning of type I collagen and simultaneous electrostatic spinning A report on evenly distributing mineralized glue nanoparticles in collagen fibers by spraying

Method used

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  • Method for preparing guided tissue regeneration membrane through combination of electrostatic spinning and electrostatic spraying
  • Method for preparing guided tissue regeneration membrane through combination of electrostatic spinning and electrostatic spraying
  • Method for preparing guided tissue regeneration membrane through combination of electrostatic spinning and electrostatic spraying

Examples

Experimental program
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Effect test

preparation example Construction

[0071] 1. Preparation of Mineralized Collagen Powder

[0072] 1.1. Dissolve type I collagen in any one of hydrochloric acid, nitric acid or acetic acid to prepare an acid solution of type I collagen. The concentration of type I collagen contained in the prepared type I collagen acid solution is 0.01-0.2g / mL;

[0073] 1.2. Add calcium salt solution dropwise to the type I collagen acid solution, wherein the amount of calcium ions added is 0.1-2 mol of calcium ions corresponding to each gram of type I collagen;

[0074] 1.3. Add phosphoric acid solution dropwise in the solution obtained in step 1.2, wherein the molar ratio of the amount of phosphate ions added to the amount of calcium ions added in step 1.2 is Ca / P=1 / 1~2 / 1;

[0075] 1.4. Add NaOH solution dropwise to the solution obtained in step 1.3 to form a mixed solution, and adjust the pH value to 6-8;

[0076] 1.5. After the mixed solution obtained in step 1.4 was left to stand for 4-12 hours, the precipitate was centr...

Embodiment 1

[0105] 1. Preparation of Mineralized Collagen Powder

[0106] 1.1. Dissolve type I collagen in hydrochloric acid, and the concentration of type I collagen contained in the prepared type I collagen acid solution is 0.1g / mL;

[0107] 1.2. Add a calcium salt solution dropwise to the type I collagen acid solution, wherein the amount of calcium ions added is 1 mol of calcium ions corresponding to each gram of type I collagen;

[0108] 1.3, drop phosphoric acid solution in the solution gained in step 1.2, wherein the mol ratio of the addition of phosphate ions to the addition of calcium ions in step 1.2 is Ca / P=1.5 / 1;

[0109] 1.4. Add NaOH solution dropwise to the solution obtained in step 1.3 to form a mixed solution, and adjust the pH value to 6.5;

[0110] 1.5. After the mixed solution obtained in step 1.4 was left to stand for 8 hours, the precipitate was centrifuged at a speed of 4000 r / min, and then air-dried at 60°C for 60 hours to obtain mineralized collagen particles; ...

Embodiment 2

[0138] Embodiment 2 is basically the same as Embodiment 1, the difference is:

[0139] In the preparation of type I collagen electrospinning solution in step 2.2:

[0140] Using trifluoroacetic acid as a solvent, weigh type Ⅰ collagen and add it into it, stir and dissolve overnight, to prepare 8w / v% type Ⅰ collagen electrospinning solution.

[0141] In the preparation of the nano-mineralized collagen electrostatic spray liquid in step 2.3:

[0142] Weigh the mineralized collagen powder and add it to methanol solution (methanol water solution with a volume percentage of 35%) to obtain a 6w / v% suspension, and ultrasonically treat the suspension at a frequency of 20kHz for 1h to obtain mineral Collagen electrostatic spray solution.

[0143] In the preparation of the rough layer in step 3.3:

[0144] Fix the syringe containing the mineralized collagen electrostatic spray solution on the micro-syringe pump. The inner diameter of the syringe is 14.9 mm, and each is connected with...

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Abstract

The invention relates to a method for preparing a guided tissue regeneration membrane by combining electrostatic spinning and electrostatic spraying. The method comprises the following steps: carrying out electrostatic spinning on a PLCL electrostatic spinning solution to prepare a smooth layer; the PLCL electrostatic spinning solution and the I-type collagen electrostatic spinning solution are subjected to electrostatic spinning opposite spinning, and a middle layer is prepared on the surface of the smooth layer; the I-type collagen electrostatic spinning solution is subjected to electrostatic spinning, meanwhile, the mineralized collagen electrostatic spraying solution is subjected to electrostatic spraying, a rough layer is prepared on the surface of the middle layer, and then the guided tissue regeneration membrane is prepared through drying and cross-linking. The prepared guided tissue regeneration membrane can play an isolation barrier role, has certain guided tissue regeneration performance, is beneficial to adhesion, proliferation and differentiation of cells, can provide space for cell crawling along with degradation of collagen, and is excellent in mechanical performance, operability and the like.

Description

technical field [0001] The invention belongs to the technical field of medical device materials, and in particular relates to a method for preparing a guiding tissue regeneration membrane by combining electrospinning and electrostatic spraying. Background technique [0002] Dental implants have become an important means of repairing dentition defects and loss, but due to the natural atrophy of the alveolar ridge, trauma, periodontal disease and other reasons, the lack of bone mass reduces the success rate of implant restoration and limits the effectiveness of dental implants. The scope of application, and the application of guided tissue regeneration (GTR) can effectively solve the problems of insufficient bone mass and alveolar bone defect. This technology is to place a barrier membrane in the bone defect to prevent fibroblasts from entering the bone defect, and at the same time, it should be able to promote the adhesion, proliferation and bone tissue regeneration of osteob...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/04A61L31/06A61L31/14D04H1/728D01D5/00
CPCA61L31/06A61L31/044A61L31/148A61L31/042A61L31/047D04H1/728D01D5/0007A61L2400/12C08L67/04C08L89/00
Inventor 崔云宋天喜胡艳丽何志敏朱金亮胡刚仇志烨吴晶晶
Owner BEIJING ALLGENS MEDICAL SCI & TECH
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