Lactobacillus plantarum SD21, screening method and application thereof
A technology of Lactobacillus plantarum, SD21, applied in the field of microorganisms, can solve problems such as unfavorable oral health, imbalance of microbial system, enhanced bacterial resistance, etc., and achieve the effect of good application potential, high safety, and high commercial value.
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Embodiment 1
[0060] The isolation of embodiment 1 lactobacillus plantarum SD21 bacterial strain
[0061] From the farm pickle samples aseptically collected in Daliangshan, Sichuan, 5g of different samples were taken by the coating plate method, put into sterile homogeneous bags, marked, and 45mL of 0.85% normal saline was added respectively, and then patted and mixed completely. Then draw 100 μL of sample for 10-fold serial serial dilution, and draw the dilution factor to be 10 -3 、10 -4 、10 -5 、10 -6 100 μL of the sample, coated in 2.5% CaCO 3 MRS plates were cultured upside down at 37°C for 24 hours. Pick colonies with good growth and large calcilytic circle (such as figure 1 ), separated and purified repeatedly by plate streak separation method (such as figure 2 ), until a single colony was obtained, the isolated strain was named SD21, numbered, and stored in glycerol at -80°C.
Embodiment 2
[0062] Identification of embodiment 2 Lactobacillus plantarum SD21 bacterial strain
[0063] (1) Physiological and biochemical tests
[0064] Gram staining (such as image 3 ) and catalase test, and its physiological and biochemical indicators were measured, and the test results were compared with "Bergey's Systematic Bacteriology Manual Eighth Edition" for preliminary identification of bacterial species. The test shows that the Gram stain of the screened strain SD21 is purple and positive. The cell shape is rod-shaped, catalase and oxidase are negative, and there is no spore formation. The physical and chemical test results are shown in Table 2.
[0065] Table 2 shows the cell morphology and physicochemical test results of Lactobacillus plantarum SD21
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[0068] Note: + means positive, and this substrate can be used for fermentation; - means negative.
[0069] (2) 16S rRNA identification
[0070] According to the instructions of the bacterial gene D...
Embodiment 3
[0084] The mensuration of the growth curve of embodiment 3 Lactobacillus plantarum SD21
[0085] (1) Preparation of fermentation broth
[0086] Lactobacillus plantarum SD21 stored at -80°C was inoculated into MRS liquid medium, cultured at 37°C for 24 hours, and continuously transferred twice to complete the activation of the strain. Then the activated Lactobacillus plantarum SD21 was inoculated in 200mL MRS liquid medium at a volume of 2%, shaken evenly, cultured at 37°C for 24h, centrifuged at 4°C, 10 000r / min for 15min to obtain the fermentation broth.
[0087] (2) Determination of growth curve
[0088] Take the Lactobacillus plantarum SD21 activated for one generation, inoculate it in 200mL MRS liquid medium at a volume of 2%, oscillate evenly, place it on a shaker at 37°C at 180r / min for cultivation, take samples every 1h, measure its OD600 nm, and draw Growth curve of Lactobacillus plantarum SD21. from Figure 5 It can be obtained that Lactobacillus plantarum SD21 en...
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