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Preparation method of calcium carbonate nano-drug

A nanomedicine, calcium carbonate technology, applied in the field of preparation of calcium carbonate nanomedicine, can solve problems such as unstable bioavailability, achieve the effect of facilitating cell phagocytosis, ensuring biocompatibility, and simple preparation method

Pending Publication Date: 2022-02-18
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the shortcomings of easy degradation, instability and limited bioavailability, the application of GSDMD protein in the field of cancer therapy has only made limited progress, so it is imminent to construct a nano-delivery system that can stabilize GSDMD protein

Method used

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  • Preparation method of calcium carbonate nano-drug
  • Preparation method of calcium carbonate nano-drug
  • Preparation method of calcium carbonate nano-drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: preparing a kind of nano-calcium carbonate particle not loaded with medicine comprises the following steps.

[0027] (1) Dissolve 60 mg of calcium chloride in 20 mL of deionized water to prepare a 3 mg / mL calcium chloride solution; dissolve 200 mg of sodium carbonate in 1 L of deionized water to prepare a 0.2 mg / mL sodium carbonate solution.

[0028] (2) First put the dialysis bag with a molecular weight cut-off of 100kDa, MD24 (US spectrumlabs, product number 131417) into the dialysis bag with a molecular weight cut-off of 100kDa, MD31 (US spectrumlabs, product number 131420,), and then add Fill the space between the two dialysis bags with 10 mL of deionized water, and finally transfer the calcium chloride solution to the inner dialysis bag. Put the double dialysis system into the sodium carbonate solution prepared above for dialysis for 12 hours.

[0029] (3) In order to remove free calcium chloride and sodium carbonate, put the crude product that has b...

Embodiment 2

[0031] Embodiment 2: preparing a kind of nano-calcium carbonate particle not loaded with medicine comprises the following steps.

[0032] (1) Dissolve 60 mg of calcium chloride in 20 mL of deionized water to prepare a 3 mg / mL calcium chloride solution; dissolve 200 mg of sodium carbonate in 1 L of deionized water to prepare a 0.2 mg / mL sodium carbonate solution.

[0033] (2) First put the dialysis bag with a molecular weight cut-off of 100kDa, MD24 (US spectrumlabs, product number 131417) into the dialysis bag with a molecular weight cut-off of 100kDa, MD31 (US spectrumlabs, product number 131420), and then add 10mL between the two dialysis bags Deionized water 3, finally transfer the calcium chloride solution to the inner dialysis bag. Put the double dialysis system into the above prepared sodium carbonate solution for dialysis for 24 hours.

[0034] (3) In order to remove free calcium chloride and sodium carbonate, the crude product dialyzed in step (2) was continued to be ...

Embodiment 3

[0036]Embodiment 3: the calcium carbonate that prepares a kind of calcium-loaded bovine serum albumin (bovine serum albumin is produced from Aladdin) there medicine comprises the following steps (because pyroptosis protein and bovine serum albumin molecular weight are similar, similar in structure, before loading pyroptosis protein Use calcium carbonate-loaded bovine serum albumin for preliminary experiments). The protein is fluorescently labeled with FITC. The labeling process: first dissolve 2g of bovine serum albumin in 10mL of deionized water, 10mg of FITC in 1mL of deionized water, then slowly add the FITC solution into the bovine serum albumin solution and stir in the dark 8h. After the reaction was completed, the mixed solution was transferred into a dialysis bag with a molecular weight cut-off of 1W, and dialyzed in a beaker to remove free FITC, and dialyzed for more than 12 hours. Take the liquid in the beaker for UV absorption test, if there is no absorption peak at...

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Abstract

The invention provides a preparation method of a calcium carbonate nano-drug, and the method comprises the following steps of: uniformly mixing a calcium chloride aqueous solution and a protein drug, pouring the obtained mixed solution into a dialysis bag A, then putting the dialysis bag A into a dialysis bag B larger than the dialysis bag A, filling the space between the dialysis bag A and the dialysis bag B with deionized water, performing sealing, then placing the dialysis bags in a sodium carbonate aqueous solution, conducting dialyzing while stirring, and conducting post-treatment on the obtained reaction liquid to obtain the calcium carbonate nano-drug. The calcium carbonate nanoparticles are prepared by using a method of controlling ion diffusion by using a double-layer dialysis bag, the preparation method is simple, the particle size is uniform, substances such as a surfactant and a polymer for controlling the growth of calcium carbonate grains are not used, and the biocompatibility of the nano calcium carbonate is ensured to the greatest extent.

Description

technical field [0001] The invention relates to the preparation of calcium carbonate nano medicine. Background technique [0002] Among inorganic materials, materials such as calcium carbonate, calcium phosphate, silicon dioxide, and iron oxide can be used as drug carriers due to their excellent properties and are widely used in the field of medicine. Compared with other inorganic materials, CaCO 3 Nanoparticles have a variety of size, porosity, crystallization and morphology, so they have unique advantages as drug carriers. In addition, calcium carbonate is pH-sensitive, and it remains stable in a neutral environment, but it will produce a certain degree of microdissolution and destruction of the structure in a weak acid environment, and the pH of the tumor tissue microenvironment is lower than that of normal tissue microenvironment, so The drug carrier based on calcium carbonate has certain low pH responsiveness. However, in conventional chemical synthesis, calcium carb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/02A61K38/17A61K38/38B82Y5/00B82Y40/00
CPCA61K47/02A61K38/1709A61K38/385B82Y5/00B82Y40/00
Inventor 吴丹张占魁胡青莲
Owner ZHEJIANG UNIV OF TECH
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