Preparation method of hydrogel material for rapid hemostasis

Abstract

The invention relates to a preparation method of a hydrogel material for rapid hemostasis, which comprises the steps of mixing cysteine and o-nitrobenzyl micromolecule condensation modified polysaccharide, acrylic acid modified gelatin, hydrogen peroxide and 1-ethyl-(3-dimethylaminopropyl) carbodiimide salt in a PBS (Phosphate Buffer Solution) to obtain a mixed solution A; enabling the mixed solution A to be subjected to a standing reaction for 12-48 h at 20-35 DEG C under nitrogen protection, and forming a polysaccharide-gelatin preliminary cross-linked hydrogel precursor solution, wherein the mixed solution A is prepared from the following components in percentage by mass: 1 to 3 percent of modified polysaccharide, 3 to 8 percent of acrylic modified gelatin, 0.2 to 0.8 percent of hydrogen peroxide and 0.05 to 0.2 percent of 1-ethyl-(3-dimethylaminopropyl) carbodiimide salt; and enabling the primarily crosslinked hydrogel precursor solution to be subjected to a crosslinking reaction under the illumination condition with the wavelength of 260-430 nm to obtain the hydrogel material. The hydrogel material is high in gel forming speed and high in gel mechanical strength, and can be used for rapid hemostasis of arteries and the like.

Description

technical field [0001] The invention belongs to the technical field of medical hemostatic materials, and in particular relates to a preparation method of a hydrogel material for rapid hemostasis. Background technique [0002] Uncontrolled bleeding in the medical field of trauma surgery is the leading cause of the highest fatality rate. The main reason for the inability to effectively stop bleeding is that repairing aortic rupture and suturing hemostasis for penetrating heart injuries are extremely difficult surgical challenges. However, this cannot be performed outside the surgical unit and has great limitations. With recent advances in materials science, many experimental chemical agents have been tested for rapid wound sealing, such as fibrin glue, gelatin, collagen, oxidized cellulose, zeolites, peptides, polymers, and hydrogels, however, although emerging Some materials are suitable for hemostasis and sealing of aortic and cardiac trauma, but most of them have low hemos...

AI Technical Summary

Problems solved by technology

The main reason for the inability to effectively stop the bleeding is that the repair of the aortic rupture and the suturing of the penetrating heart to stop the bleeding are extremely difficult surgical challenges. However, this cannot be performed outside the surgical unit and has great limitations.

With recent advances in materials science, many experimental chemical agents have been tested for rapid wound sealing, such as fibrin glue, gelatin, collagen, oxidized cellulose, zeolites, peptides, polymers, and hydrogels, however, although emerging Some materials are suitable for hemostasis and sealing of aortic and cardiac trauma, but most of them have low hemostatic performance, slow hemostatic speed, poor adhesion on wet tissue surfaces, weak or inflexible adhesion, and are difficult to apply in biological tissues that are refractory to degradation and produce toxic substances

Images