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Toothpaste capable of efficiently inhibiting helicobacter pylori as well as preparation method and application of toothpaste

A technology of Helicobacter pylori and toothpaste, applied in the toothpaste for efficiently inhibiting Helicobacter pylori and its preparation field, can solve problems such as large-scale production of unfavorable IgY antibodies, difficulty in large-scale culture, harsh growth conditions, etc., and achieve excellent antibacterial efficacy , low cost, strong antibacterial effect

Pending Publication Date: 2022-02-22
江苏安泰生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this application, Helicobacter pylori strains were used as the immunogen to prepare IgY antibodies. Since Helicobacter pylori has very harsh requirements on growth conditions, large-scale cultivation is difficult, which is not conducive to the large-scale production of IgY antibodies.
Moreover, Moringa is mainly planted in tropical regions, and the cost of Moringa leaves is relatively high

Method used

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  • Toothpaste capable of efficiently inhibiting helicobacter pylori as well as preparation method and application of toothpaste
  • Toothpaste capable of efficiently inhibiting helicobacter pylori as well as preparation method and application of toothpaste

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] The preparation of embodiment 1 egg yolk globulin powder

[0059] Select 20-week-old hens, mix and fully emulsify the monoepitope antigen peptide shown in SEQ ID NO: 2 with Freund's complete adjuvant in equal volumes, and treat the breast muscle and thigh root of the hen at a dose of 1ml / hen Multi-point injection; every two weeks, use the monoepitopic antigen peptide or dendritic epitope peptide and Freund's incomplete adjuvant to mix equal volumes and fully emulsify the immunogen to boost the immunization once, the immunization dose is 1ml / body, A total of 3 booster immunizations were performed; eggs were collected 2 weeks after the last booster immunization, and unimmunized eggs were collected as negative controls.

[0060] Remove the sterilized eggs, collect the yolk and puncture it, measure 10ml egg yolk liquid, add 10 times of double distilled water to dilute, mix well, adjust the pH value to 4-5, overnight at -20°C, thaw at room temperature, 12000rpm at 4°C Cent...

Embodiment 2

[0061] The preparation of embodiment 2 young barley juice powder

[0062] After cleaning and draining the fresh barley seedlings, use a vacuum freeze dryer for freeze-drying, the pre-freezing temperature is -45°C, the cold trap temperature is -37°C, the sublimation temperature is 25°C, and the vacuum degree is 170Pa; Mesh sieve; take 5.0g sample and place it in a 250ml conical flask, add 95% (v / v) ethanol solution to extract, the ratio of solid to liquid is 25:1, seal the conical flask with a sealing film and place it in a shaker In the bed, extract at 37°C and 100rpm for 60min; after the extraction is completed, centrifuge the extract at 10,000rpm at 4°C for 10min, and take the supernatant to obtain the product.

Embodiment 3

[0063] The preparation of embodiment 3 mulberry leaf juice powder

[0064] Clean and dry the fresh mulberry leaves, cut them into pieces, add pure water according to the material-to-liquid ratio of 1:30, mix well, and conduct ultrasonic treatment with ultrasonic power of 500W and ultrasonic time of 20-30min; after ultrasonic treatment, add an equal volume of absolute ethanol for Leaching, leaching at 50°C and 180rpm for 120min; centrifuging the extract at 12000rpm at 4°C for 8min, and taking the supernatant to obtain the product.

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Abstract

The toothpaste containing the helicobacter pylori specific yolk protein and the natural plant extract, provided by the invention, has a stronger antibacterial effect while cleaning teeth.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a toothpaste for efficiently inhibiting Helicobacter pylori, a preparation method thereof and an application thereof. Background technique [0002] Helicobacter pylori (H. pylori) is a Gram-negative bacillus with a high infection rate, which can cause gastritis, gastric ulcer and even gastric cancer. Only a small number of infected persons will develop clinical symptoms, while most asymptomatic carriers are often ignored, which indirectly leads to the widespread transmission of Helicobacter pylori among the population. Therefore, it is necessary to develop new biological products for the prevention and treatment of H. pylori infection. [0003] CN110507583A discloses a fresh breath toothpaste for preventing and controlling Helicobacter pylori, comprising hydrated silica, cellulose gum, sodium lauryl sulfate, Helicobacter pylori type IgY, Moringa leaf extract, sorbitol (s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/40A61P31/04C07K16/12C07K16/02A61K38/40A61K36/8998
CPCA61K39/40A61K38/40A61K36/8998A61P31/04C07K16/1203C07K16/02A61K2300/00
Inventor 闫小君罗进
Owner 江苏安泰生物技术有限公司
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