Ganoderma lucidum strain GZ and application thereof

A technology of Ganoderma lucidum and bacterial strains, which is applied in the field of Ganoderma lucidum strains, can solve the problems of difficulty in meeting the market requirements of medicine and health food, low levels of polysaccharides and triterpenes, and achieve real-time and on-site detection, shortened cultivation period, and detection methods simple effect

Active Publication Date: 2022-02-25
GUIZHOU INST OF SOIL & FERTILIZER +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are still relatively few ganoderma strains used for artificial cultivation in the Chinese market, and the content of polysaccharides and triterpenes in the fruiting bodies of ganoderma lucidum in the current strains is relatively low, which is difficult to meet the market requirements for people's medicinal and health food

Method used

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  • Ganoderma lucidum strain GZ and application thereof
  • Ganoderma lucidum strain GZ and application thereof
  • Ganoderma lucidum strain GZ and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039] The collection of embodiment 1 ganoderma lucidum strain GZ of the present invention, separation domestication process:

[0040] In August 2016, the inventor discovered a wild Ganoderma lucidum in Kuankuanshui Nature Reserve, Suiyang, Guizhou. The fruiting body tissue of wild Ganoderma lucidum is separated to obtain mycelia (bacteria), and then the isolated strains are subjected to a fruiting test, and then the fruiting bodies with good growth are separated, and the strains with good growth are screened to continue cultivation and domestication. After several generations of cultivation, a Ganoderma lucidum strain with fast mycelial growth and neat fruiting was selected and named: GZ.

Embodiment 2

[0041]The classification identification of embodiment 2 bacterial strain Ganoderma lucidum strain GZ of the present invention:

[0042] (1) Morphological identification of Ganoderma lucidum bacterial strain GZ of the present invention:

[0043] Fruiting body annual, with lateral stalk, corky. The cap is flat, semicircular or fan-shaped, and the size of the cap is 6.1-10.9 cm. The surface of the cap is yellowish brown when it is fresh, with obvious luster, concentric rings, and blunt edges. The surface of the pores is white when fresh, light brown when dry. The flesh is light brown, from light to dark from top to bottom. According to the photographs and corresponding feature descriptions of Ganoderma lucidum (Ganoderma lucidum) on the 990th page of "Chinese Large-scale Fungal Resources Illustrated Book" (Edited by Li Yu, Zhongyuan Farmer's Publishing House, published in 2015), the morphological description of Ganoderma lucidum strain GZ of the present invention is similar to ...

Embodiment 3

[0051] The cultivation test of embodiment 3 ganoderma lucidum bacterial strain GZ of the present invention:

[0052] (1) Mother species cultivation: inoculate the ganoderma lucidum strain GZ strain on the mother species culture medium, and cultivate for 8 to 10 days at 22-25° C., relative air humidity of 70%, and dark conditions to obtain the ganoderma lucidum strain GZ mother species; The preparation method of the mother seed medium: peel 200g of potatoes and cut them into 1.5cm squares, put them into 800mL water and boil for 20min, filter through 3 layers of gauze, take the extract, add 20g of glucose, 18g of agar, potassium dihydrogen phosphate 2g, magnesium sulfate 1g, vitamin B 1 1g, mix well and dilute to 1000mL, sterilize at 121°C for 30min; pour into a plate with a diameter of 90mm, and set aside.

[0053] (2) Original seed culture: Inoculate the GZ mother seed obtained in step (1) on the original seed medium according to the ratio of 1:35, and cultivate it for 30 day...

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Abstract

The invention belongs to the field of edible mushrooms, and particularly discloses a Ganoderma Lucidum strain GZ with an accession number of CCTCC NO: M2019248. The invention further discloses application of the strain in the aspects of food, health care products, beverages and the like. The Ganoderma lucidum strain GZ is attractive in appearance and high in sporocarp commodity value, and the varieties of cultivated Ganoderma lucidum are enriched; secondly, the cultivation period of the strain is shortened by 5-130 days compared with the cultivation period of an existing known ganoderma lucidum strain, and the cultivation period is greatly shortened; and besides, the polysaccharide content of the strain is as high as 1.7%, the content of triterpene and sterol is as high as 1.6%, and the strain is high in medicinal value, can be used for preparing health-care food for improving human immunity or anti-tumor medicines, and is very wide in application prospect. The invention also discloses a molecular marker of the strain. The molecular marker has strong specificity to the strain GZ, and an identification result is accurate and reliable; and a detection method is simple and rapid, and is suitable for real-time and field detection.

Description

technical field [0001] The invention belongs to the field of edible fungi, and in particular relates to a ganoderma strain, and also relates to the application of the ganoderma strain. Background technique [0002] Ganoderma lucidum (also known as Ruicao, Shenzhi, Xiancao, Yaocao, Huanyangcao, Lin Zhongling, Ganoderma lucidum, Wannian mushroom, Lingcao, Chizhi, Danzhi or Qiongzhen, etc.) belongs to the polyporaceae Ganoderma lucidum ( Ganoderma lucidum). Ganoderma lucidum has high medicinal value. For example, Ganoderma lucidum polysaccharides can improve immunity, lower blood sugar, lower blood fat, anti-oxidation, anti-aging and anti-tumor effects; triterpenoids in Ganoderma lucidum can purify blood and protect liver function; A variety of preparations have sedative, anticonvulsant, cardiotonic, antiarrhythmic, antihypertensive, antitussive and antiasthmatic effects; Ganoderma lucidum also has anticoagulation, inhibition of platelet aggregation and antiallergic effects. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12Q1/6895C12N15/11A61K36/074A61P35/00A61P37/04A23L31/00A23L33/00A23L2/52A01G18/00A01G18/20A01G18/40C12R1/645
CPCC12Q1/6895A61K36/074A61P35/00A61P37/04A23L31/00A23L33/00A23L2/52A01G18/00A01G18/20A01G18/40A23V2002/00A23V2250/208A23V2200/324
Inventor 杨杰仲王晓敏杜慕云张邦喜陈旭朱森林杨珍陈波张钦语魏善元唐玲杨仁德
Owner GUIZHOU INST OF SOIL & FERTILIZER
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