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Preparation method of millet protein and millet protein hydrolysate and application of millet protein hydrolysate

A protein hydrolyzate, millet technology, applied in the preparation methods of peptides, chemical instruments and methods, hydrolyzed protein components, etc., can solve the problems of unknown prevention and treatment of IBD, application research, etc., to enhance the barrier function of intestinal epithelial cells, no Side effects, effects of suppressing expression

Pending Publication Date: 2022-03-01
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether the intake of millet protein hydrolyzate can prevent and treat the progress of IBD remains unknown
Previous studies have shown that regular consumption of millet and other grain porridge has an auxiliary effect on enteritis, but there is no research on its application as a drug

Method used

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  • Preparation method of millet protein and millet protein hydrolysate and application of millet protein hydrolysate
  • Preparation method of millet protein and millet protein hydrolysate and application of millet protein hydrolysate
  • Preparation method of millet protein and millet protein hydrolysate and application of millet protein hydrolysate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Preparation of millet protein

[0032] The millet is first ground and degreased.

[0033] (1) Grind millet with an ultrafine pulverizer, pass the obtained millet flour through a 60-mesh sieve, weigh 100 g of millet flour and n-hexane according to a material-to-liquid ratio of 1:10 (w / v), mix it with ultrasonic extraction for 1 hour, centrifuge at 2000 g, discard supernatant, and repeat the above operation once for the remaining residue, and place the collected residue in a fume hood to air-dry naturally to obtain defatted millet flour.

[0034] (2) Weigh the defatted millet flour obtained by the method in (1) and ultrapure water according to the ratio of material to liquid 1:6 (w / v), mix and fully stir, adjust to pH=7.0 with 1mol / L NaOH, add 30U / L g of α-amylase, hydrolyze in a water bath at 50°C for 2 hours, and keep stirring during the period to make the system uniform. After the reaction, the enzyme was inactivated in a boiling water bath for 20 minutes, cooled ...

Embodiment 2

[0042]The preparation of embodiment 2 millet protein hydrolyzate

[0043] (1) Weigh the millet protein A prepared previously and mix it with ultrapure water according to the material-to-liquid ratio of 1:50 (w / v),

[0044] (2) Adjust pH to 2.0 with 1mol / L HCL.

[0045] (3) Add pepsin (500U / mg) according to enzyme / substrate=1:70.

[0046] (4) Enzymolysis in a water bath at 37°C for 2 hours.

[0047] (5) Inactivate the enzyme activity in a boiling water bath for 20 minutes, and cool to room temperature naturally.

[0048] (6) Adjust pH to 7.5 with 1mol / L NaOH, and add trypsin (4×USP) according to enzyme / substrate = 1:10.

[0049] (7) Enzymolysis in water bath at 37°C for 2 hours.

[0050] (8) The enzyme was inactivated in a boiling water bath for 20 minutes, and cooled to room temperature naturally.

[0051] (9) The hydrolyzed sample was centrifuged at 8000 g for 20 min, and the supernatant was collected.

[0052] (10) freeze-drying to obtain millet protein hydrolyzate D, ...

Embodiment 3

[0076] Example 3 Effect of Millet Protein Hydrolyzate on Apparent Physiological Indexes of Colitis Mice

[0077] Thirty-six 4-week-old SPF male BALB / c mice were raised in the Animal Breeding Center of Nankai University, feeding conditions: 25±2°C, relative humidity 50±5%, 12-hour cycle light, free access to water and food, related experimental operations Comply with the ethical requirements of animal experimentation. The common feed was fed for 1 week to adapt to the environment, and the experiment officially started from the second week.

[0078] In this experiment, a mouse model of colitis induced by 3% DSS was used. After a one-week adaptation period, 36 mice were randomly divided into normal group (NM), model group (DSS), and millet protein treatment group (FMPH), with 12 mice in each group and 4 mice in each cage. After one week of adaptation, the mice in the FHPH group were given 400 mg / kg·day millet protein hydrolyzate solution, and the mice in the normal group and th...

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Abstract

The invention discloses a preparation method of millet protein, and further discloses a method for preparing millet protein hydrolysate by using the millet protein through hydrolysis, and application of the millet protein hydrolysate to preparation of medicines for treating or improving colitis. The millet protein hydrolysate has the beneficial effects that the millet is a cereal food with homology of medicine and food, the extracted millet protein hydrolysate is used for preparing medicines for treating or improving colitis and intestinal barrier function impairment, can effectively inhibit expression of intestinal proinflammatory cytokines and enhance the intestinal epithelial cell barrier function, and is remarkable in effect, free of toxicity and free of side effects after being taken for a long time.

Description

technical field [0001] The invention belongs to the technical field of using plant extracts to prepare medicines, in particular to a preparation method of millet protein hydrolyzate and medicines for treating or improving colonic inflammation. Background technique [0002] Inflammatory bowel disease (IBD) is a chronic gastrointestinal disease characterized by diarrhea, abdominal pain, and blood in the stool, which seriously affects the quality of life of patients. IBD includes two subtypes, ulcerative colitis (UC) and Crohn's disease (CD). Epidemiological studies have shown that with the increasing prevalence year by year, IBD has become one of the major gastrointestinal diseases in the western world and even in Asia, Africa and South America. To date, although the exact mechanism of IBD pathogenesis is not fully understood, abnormal immune response and disruption of intestinal barrier function are considered to be the main causes of IBD. Gut barrier function is maintained...

Claims

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Application Information

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IPC IPC(8): C07K1/14C12P21/06A61K38/01A61P1/00
CPCC07K1/145C12P21/06A61K38/011A61P1/00
Inventor 王硕张博崴许樱川王津
Owner NANKAI UNIV
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