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Method for measuring content of resistant starch in rice in batches

A technology for resistant starch and rice, which is applied in the preparation of test samples, biochemical equipment and methods, and determination/inspection of microorganisms, etc. and other problems, to achieve the effect of saving measurement costs, realizing batch operations, and taking a long time.

Active Publication Date: 2022-03-04
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the process of dissolving resistant starch in KOH solution, the starch emulsification will be caused by vortex mixing; rice RS will form insoluble starch lumps, which will affect the measurement effect; this solution also has high cost and cannot measure rice RS content in large quantities. technical flaws

Method used

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  • Method for measuring content of resistant starch in rice in batches
  • Method for measuring content of resistant starch in rice in batches
  • Method for measuring content of resistant starch in rice in batches

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Experimental program
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Embodiment 1

[0050] Embodiment 1, a kind of method for measuring rice resistant starch content in batches:

[0051] 1. Materials and Instruments

[0052] Sample rice noodles to be tested; constant temperature oscillating shaker, desktop high-speed refrigerated centrifuge, mini centrifuge, oven, steel ball (diameter 6mm), automatic sample rapid grinder, micro vortex mixer, UV-visible spectrophotometer.

[0053] 2. Reagent preparation

[0054] ①Sodium maleate buffer solution (0.1M, pH6.0): Take 11.6g sodium maleate (AR, ≥99.0%, HPLC, CASNO: 110-16-7), dissolve it in 800mL distilled water, and dissolve it with sodium hydroxide (4M) to adjust the pH to 6.0, then add 0.3 g of calcium chloride, dilute to 1 L with distilled water, and store at 4°C for later use.

[0055] ②AMG Diluent 1 (300U / mL): Take 0.4mL AMG solution (Megazyme Company, Cat. No. K-RSTAR, containing enzyme 3300U / mL) in a centrifuge tube, and use the above-mentioned sodium maleate buffer solution (0.1M, pH6.0 ) to 4.4mL and st...

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Abstract

The invention discloses a method for measuring the content of resistant starch in rice in batches, which utilizes a double-enzyme hydrolysis method to measure the content of the resistant starch in the rice, that is, firstly, non-resistant starch is hydrolyzed into glucose by pancreatic alpha-amylase, then the resistant starch is dissolved by steel balls and KOH, then the resistant starch is hydrolyzed into glucose by an amyloglucosidase (AMG) solution, and finally, the content of the resistant starch in the rice is measured. And determining the glucose content by using a GOPOD method, so as to calculate the content of the resistant starch according to the standard curve. Compared with a traditional AOAC method which is long in time consumption, low in efficiency, high in cost and the like, the method for measuring the content of the resistant starch in the rice in batches has the advantages of being low in cost, good in repeatability, high in accuracy, capable of achieving batch operation and the like.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection of resistant starch content in rice, and in particular relates to a method for detecting the content of resistant starch in rice. Background technique [0002] Rice is an extremely important food crop. More than 60% of the population in my country takes rice as a staple food, and more than 1 / 3 of the world's population takes rice as a staple food. The GI value of rice varieties varies from 48 to 93. Rice and its products are mainly sold in It is digested by amylase in the stomach and small intestine, and the final product is glucose, so rice is considered a high glycemic index (Glycemicindex, GI) food; [0003] Resistant starch (RS) is a general term for starch and starch degradation products that can avoid the hydrolysis of pancreatic α-amylase in the healthy small intestine, which are not digested and absorbed, and were named by Englyst in 1983. RS has similar physiological functi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N1/28C12Q1/40C12Q1/54
CPCG01N21/78G01N1/28G01N1/286C12Q1/40C12Q1/54G01N2001/2866G01N2333/934
Inventor 罗嘉锐竺正航郝晴文杨维丰姚惠珂
Owner SOUTH CHINA AGRI UNIV
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