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Antibacterial peptide composition and application thereof

A technology of antimicrobial peptides and compositions, which is applied in the field of antimicrobial peptide compositions, can solve the problems of large cationic antibacterial peptides, limited effects, and toxic effects, and achieve good antibacterial activity, improved antibacterial efficiency, and good application prospects

Active Publication Date: 2022-03-08
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, antimicrobial peptides still face many unresolved problems as antibacterial drugs: some cationic antimicrobial peptides can produce greater toxicity to mammalian cells; the amphipathic structure requires a certain number of basic amino acids in the amino acid sequence, and the The property that permanent amino acids are easily hydrolyzed by proteases greatly limits their effects as drugs after they enter the human body

Method used

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  • Antibacterial peptide composition and application thereof
  • Antibacterial peptide composition and application thereof
  • Antibacterial peptide composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Configuration of MAMP

[0019] Chemically synthesized polypeptides MAMP-01 (97.7% pure), MAMP-02 (95.4% pure) and MAMP-03 (97.6% pure) were custom-made in Shanghai Jietide Technology Co., Ltd. To configure antimicrobial peptide combination MAMP: 1 g MAMP-01+10 g MAMP-02+20 g MAMP-03. Taking Escherichia coli and Porphyromonas gingivalis among Gram-negative bacteria, Staphylococcus aureus and Streptococcus mutans among Gram-positive bacteria, and Candida albicans as target strains, the antibacterial peptide composition MAMP was investigated. Antibacterial activity.

[0020] (2) Strain and recovery

[0021] Escherichia coli K12, Staphylococcus aureus, Porphyromonas gingivalis (P.g., ATCC33277), Streptococcus mutans (M.s., ATCC25175) and Candida albicans , ATCC10231) were purchased from the China Strain Resource Bank Collection Center, and were stored at -80 °C by the glycerol preservation method. Before the experiment, Escherichia coli was inoculated in LB broth me...

Embodiment 2

[0031] Configuration of MAMP: antimicrobial peptide composition MAMP: 1 g MAMP-01 + 5 g MAMP-03.

[0032] Other experimental methods are the same as in Example 1.

[0033] The antibacterial results for the five kinds of bacteria are shown in Table 2. The results show that the antimicrobial peptide composition MAMP has inhibitory activity against Gram-negative, Gram-positive bacteria and fungi, and the inhibitory activity for the above five kinds of bacteria is significantly better than that of single bacteria. Antimicrobial Peptides.

[0034] Table 2 Antibacterial results of antimicrobial peptide composition MAMP

[0035]

Embodiment 3

[0037] Configuration of MAMP: antimicrobial peptide composition MAMP: 1 g MAMP-01 + 6 g MAMP-02 + 15 g MAMP-03.

[0038] Other experimental methods are the same as in Example 1.

[0039] The antibacterial results for five kinds of bacteria are shown in Table 3. The results show that the antimicrobial peptide composition MAMP has inhibitory activity against Gram-negative, Gram-positive bacteria and fungi, and the inhibitory activity for the above five kinds of bacteria is significantly better than that of single Antimicrobial Peptides.

[0040] Table 3 Antibacterial results of antimicrobial peptide composition MAMP

[0041]

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Abstract

The invention relates to an antibacterial peptide composition MAMP. The composition is composed of three antibacterial peptides MAMP-01, MAMP-02 and MAMP-03, wherein the three antibacterial peptides MAMP-01, MAMP-02 and MAMP-03 are from hydrolyzed milk protein. The amino acid sequence of the MAMP-01 is Ala-Ala-Ser-Asp-Ile-Ser-Leu-Leu (AASDISLL), the amino acid sequence of the MAMP-02 is Leu-Asp-Ala-Gln-Ser-Asp-Pro-Leu-Arg (LDAQSAPLR), and the amino acid sequence of the MAMP-03 is Leu-Asp-Thr-Asp-Tyr-Lys-Lys-Tyr (LDTDYKKY), the amino acid sequence of the MAMP-03 is shown in the specification, the amino acid sequence of the MAMP-03 is shown in the specification, and the amino acid sequence of the MAMP-03 is shown in the specification. The composition MAMP has wide inhibitory activity on gram-positive bacteria, gram-negative bacteria and fungi, including escherichia coli, staphylococcus aureus, porphyromonas gingivalis, streptococcus mutans, candida albicans and the like. The compound has the advantages of natural and safe raw materials and low production cost, and can be applied to medicines, foods and daily necessities as an active component for inhibiting microbial growth.

Description

technical field [0001] The present invention relates to an antibacterial peptide composition MAMP derived from hydrolyzed milk protein and its application. Background technique [0002] Antibiotics are drugs that can resist pathogenic microorganisms and are the largest class of antibacterial and anti-inflammatory drugs. Antibiotics are substances produced by bacteria, fungi or other microorganisms in the process of life. They have the effect of inhibiting or killing pathogenic microorganisms such as bacteria and fungi. Therefore, they are widely used as anti-infective drugs for various infectious diseases. The remarkable effectiveness of antibiotics in treating infections has also led to bacterial resistance, making the treatment of some infectious diseases more difficult. Antimicrobial peptides, as a new type of antibiotics, have broad-spectrum antibacterial activities, and will not easily make microorganisms resistant to resistance, and have broad application prospects. ...

Claims

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Application Information

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IPC IPC(8): C07K7/06A61K38/08A61K8/64A23L33/18A61P1/02A61P17/00A61P31/04A61P31/10A61Q11/00A61Q17/00
CPCC07K7/06A61K38/08A61K8/64A61Q11/00A61Q17/005A61P31/04A61P31/10A61P1/02A61P17/00A23L33/18A23V2002/00A23V2200/318A23V2200/30A23V2250/5424Y02A50/30
Inventor 靳艳王方军杨建
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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