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Bacterial powder composition for relieving inflammatory colitis as well as preparation method and application thereof

A composition, colitis technology, applied in the field of microorganisms, to achieve the effect of enhancing intestinal barrier function, enhancing intestinal barrier function, and reducing the number

Active Publication Date: 2022-03-11
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although there are many probiotic products that can be used to regulate the gastrointestinal tract, there are still few products that are effective in treating inflammatory colitis, and there is an urgent need to develop more probiotics that can prevent and treat inflammatory colitis. probiotic products

Method used

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  • Bacterial powder composition for relieving inflammatory colitis as well as preparation method and application thereof
  • Bacterial powder composition for relieving inflammatory colitis as well as preparation method and application thereof
  • Bacterial powder composition for relieving inflammatory colitis as well as preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The Lactobacillus paracasei JN-1 used in the present invention was preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee on June 21, 2021, and the storage address is No. 1, Beichen West Road, Chaoyang District, Beijing No. 3, the deposit number is CGMCC No.22745.

[0039] The performance test of this Lactobacillus paracasei (Lactobacillus paracasei) JN-1 is as follows:

[0040] Artificial gastrointestinal fluid resistance test of strains:

[0041] 1. Preparation of simulated saliva, gastric juice and intestinal juice:

[0042] (1) Preparation of gastrointestinal stock solution (see Table 1)

[0043] Table 1 Preparation of simulated saliva, gastric juice and intestinal juice

[0044] drug Stock solution concentration (mol / L) Gastric fluid salt concentration (mmol / L) Intestinal fluid salt concentration (mmol / L) KCl 0.5 6.9 6.8 K H 2 PO 4

0.5 0.9 0.8 NaHCO 3

1 25...

Embodiment 2

[0071] A preparation method of Lactobacillus paracasei JN-1 bacterium powder composition for alleviating inflammatory colitis is as follows:

[0072] (1) Activation of strains: Inoculate the inoculum of Lactobacillus paracasei JN-1 in the MRS solid medium, culture at 37°C for 24 hours, and obtain a single colony of the strain;

[0073] (2) Primary seed liquid preparation: Pick a single colony of Lactobacillus paracasei JN-1 in the solid medium, inoculate it in MRS liquid medium, and culture it anaerobically at 37° C. for 24 hours to obtain the primary seed liquid;

[0074] (3) Expansion culture of strains: inoculate the primary seed liquid in the liquid MRS medium with an inoculum size of 2%, and culture it anaerobically at 37° C. for 24 hours to obtain a fermentation liquid;

[0075] (4) Bacteria collection: the fermented liquid 4000rpm / min centrifugation 10min that obtains, collects thalline, washes thalline with PBS buffer solution, resuspends thalline, centrifuges to remov...

Embodiment 3

[0079] A preparation method of Lactobacillus paracasei JN-1 bacterium powder composition for alleviating inflammatory colitis is as follows:

[0080] (1) Activation of strains: Inoculate the inoculum of Lactobacillus paracasei JN-1 in the MRS solid medium, culture at 37°C for 24 hours, and obtain a single colony of the strain;

[0081] (2) Primary seed liquid preparation: Pick a single colony of Lactobacillus paracasei JN-1 in the solid medium, inoculate it in MRS liquid medium, and culture it anaerobically at 37° C. for 24 hours to obtain the primary seed liquid;

[0082] (3) Expansion of bacterial strains: Inoculate the primary seed liquid in a liquid MRS medium with an inoculum size of 3%, and culture it anaerobically at 37° C. for 22 hours to obtain a fermentation liquid;

[0083] (4) Bacteria collection: the fermented liquid 4000rpm / min centrifugation 10min that obtains, collects thalline, washes thalline with PBS buffer solution, resuspends thalline, centrifuges to remov...

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Abstract

The invention discloses a bacterial powder composition for relieving inflammatory colitis as well as a preparation method and application thereof, lactobacillus paracasei JN-1 freeze-dried bacterial powder with strong gastrointestinal digestive system resistance and bile salt tolerance is adopted, dietary fibers and vitamins are matched, and the lactobacillus paracasei JN-1 bacterial powder composition is prepared. The lactobacillus paracasei JN-1 can increase the number of beneficial bacteria and reduce the number of pathogenic bacteria, so that the homeostasis of intestinal microbiota is recovered, and cascade reaction of inflammatory factors can be reduced; dietary fibers which can be fermented by bacteria to form short-chain fatty acids are also added, so that the effects of resisting inflammation and enhancing the intestinal barrier function are achieved; in addition, vitamins with the functions of resisting inflammation, resisting oxidation, enhancing intestinal barrier function, improving immunity and the like are also added, so that the attack and progress of IBD are further reduced.

Description

technical field [0001] The invention relates to a bacterial powder composition for relieving inflammatory colitis, a preparation method and application thereof, and belongs to the technical field of microorganisms. Background technique [0002] Inflammatory colitis (Inflammatory bowel disease, IBD) is a chronic, low-mortality intestinal disease, clinically manifested as recurrent abdominal pain, diarrhea, mucus and bloody stools. IBD exists in all ages, most of which occur in adolescence and early adulthood, and the incidence of inflammatory colitis in China has reached 3 / 10,000. Studies have shown that the number of newly diagnosed IBD patients each year is expected to increase exponentially over the next decade, making inflammatory colitis one of the major public health problems. At present, the exact etiology of IBD has not been fully clarified. Some scholars believe that it may be related to intestinal microorganisms or autoimmunity. The drugs for treating inflammator...

Claims

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Application Information

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IPC IPC(8): A61K35/747A23L33/135A61P1/00A61P29/00
CPCA61K35/747A23L33/135A61P1/00A61P29/00A23V2002/00A23V2400/165A23V2200/3204
Inventor 耿燕闫建国段文慧马晓霞许正宏史劲松贾玮玮单香芬杜学贤杨树泉梁国威任怡琳
Owner JIANGNAN UNIV
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