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Marine aspergillus, method for producing alkaloid through fermentation and application of alkaloid

An Aspergillus and alkaloid technology, applied in the field of marine Aspergillus, can solve problems such as undiscovered applications, and achieve good promotion prospects, significant effects, and anti-marine fouling effects.

Active Publication Date: 2022-03-25
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has not yet been reported that these three types of compounds are prepared by fermentation of marine Aspergillus flavus, and the application of these three compounds in inhibiting marine biofouling has not yet been found.

Method used

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  • Marine aspergillus, method for producing alkaloid through fermentation and application of alkaloid
  • Marine aspergillus, method for producing alkaloid through fermentation and application of alkaloid
  • Marine aspergillus, method for producing alkaloid through fermentation and application of alkaloid

Examples

Experimental program
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Effect test

Embodiment 1

[0056] Embodiment 1: Marine Aspergillus (Aspergillus sp.SCAU137) bacterial strain

[0057] The marine Aspergillus sp.SCAU137 strain provided by the present invention was isolated and purified by the laboratory itself, and was preserved in the Guangdong Microbial Culture Collection Center on July 27, 2020, and its preservation number is GDMCC No: 61104. The phylogenetic tree constructed by the ITS DNA sequence of the marine Aspergillus of the present invention is as follows.

[0058] The registration number of its ITS sequence on GENBANK is: MF135500. sequence listing

[0059]

Embodiment 2

[0060] Embodiment 2: Sea Aspergillus (Aspergillus sp.SCAU137) strain fermentation obtains fermentation product

[0061] Fermentation of marine Aspergillus: Use a sterile inoculation loop to inoculate the fungus Aspergillus sp.SCAU137 on the PDA agar medium. The culture temperature is 26°C, and the plate culture time is 3 days. The inoculation loop picks a certain strain from the plate and inoculates it into the Erlenmeyer flask containing the PDB liquid medium, and cultivates it on a shaker for 2 days to obtain the seed liquid, and then adds the seed liquid to the flask containing the rice solid medium. In the large triangular Erlenmeyer flask, after static cultivation for a certain period of time, the fermentation product is obtained. The composition of described rice solid medium is 400g rice, 2g yeast extract, 2g glucose, 18g sea salt, 600mL water.

Embodiment 3

[0062] Example 3: Isolation and purification of marine Aspergillus sp.SCAU137 fermentation product

[0063] The fermented product of the rice solid medium was ultrasonically crushed and then ultrasonically extracted with acetone-water at a ratio of 4:1, then the extract was concentrated under reduced pressure until no acetone remained, and the concentrated aqueous phase was extracted with an equal volume of ethyl acetate. Concentration under reduced pressure gave 30 g of ethyl acetate extract. The above ethyl acetate extract was subjected to silica gel column chromatography, and gradient elution was performed with chloroform-methanol 95:5 to obtain fraction Fr.1; gradient elution was performed with chloroform-methanol 98:2 to obtain fraction Fr.2.

[0064] Fr.1 was subjected to normal phase silica gel column chromatography, gradient elution with chloroform-acetone system to obtain the fraction Fr.1-1, and then ODS reverse column chromatography with methanol-water 7:3 to obtain...

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Abstract

The invention discloses a marine aspergillus sp. SCAU137. The marine aspergillus sp. Has the capability of generating alkaloid through fermentation, and the alkaloid has the capability of resisting marine fouling substances. According to the anti-fouling detection method for alkaloid produced by marine aspergillus, a fouling microbial population is taken as a detection object for the first time, and the anti-fouling potential of a compound can be comprehensively detected; a part of results can be identified by naked eyes, and the compound has a remarkable marine fouling resistance effect, has a good inhibition effect on the growth of biofilm-producing fouling bacteria such as psychrobacter, alteromonas, pseudomonas, vibrio, rujgera, erythrobacter and shewanella, can be used as a microecological preparation for resisting marine fouling, has good application potential and popularization prospect, and has wide application prospects. And scientific research reference basis can be provided for research and development of environment-friendly marine biological agents.

Description

technical field [0001] The present invention relates to the field of marine biological fouling removal, in particular to a marine aspergillus (Aspergillussp.SCAU137), which is fermented with the strain, and the fermentation product is separated and purified to obtain an alkaloid. The application of the preparation and the method for rapidly detecting marine pollution by using the preparation. Background technique [0002] Biofouling refers to the accumulation of marine organisms such as algae, crustaceans, etc. anywhere on the hull, including the hull, rudder, propeller and other hull accessories, or seawater systems in the ship such as seawater suction tanks, engine cooling lines, shipboard and accessories Bioaccumulation and fouling of equipment, or mobile or fixed installations such as aquaculture facilities. In recent years, with the development of shipping, coastal defense, aquaculture and seaside power plants, the harm caused by marine biofouling has become more and m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P17/18A01N43/90A01N63/34A01P1/00C12Q1/689C12Q1/6869C12R1/66
CPCC12P17/18C12P17/182A01N43/90A01N63/34C12Q1/689C12Q1/6869C12Q2535/122
Inventor 张晓勇李一阳黄日明于宗赫刘永春吴可越
Owner SOUTH CHINA AGRI UNIV
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