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Clostridium butyricum for feed additive and application of clostridium butyricum

A technology of Clostridium butyricum and additives, applied in the direction of application, microbe-based methods, bacteria, etc., can solve problems such as environmental pollution, low utilization rate of rice flour wastewater, waste of resources, etc., to reduce production costs and improve animal feed conversion rate , Improve the effect of comprehensive benefits

Pending Publication Date: 2022-03-25
南宁市拜欧生物工程有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies in the prior art, the present invention provides a Clostridium butyricum used as a feed additive and its application, which solves the problem that the utilization rate of rice flour wastewater in the production process is extremely low, and most of the rice flour wastewater and passion fruit peel It is directly discharged and thrown away as waste, causing environmental pollution and waste of resources

Method used

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  • Clostridium butyricum for feed additive and application of clostridium butyricum
  • Clostridium butyricum for feed additive and application of clostridium butyricum
  • Clostridium butyricum for feed additive and application of clostridium butyricum

Examples

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Embodiment 1

[0025] In this example, the inoculation ratio of Clostridium butyricum was investigated, and different inoculation ratios of the seed liquid of Clostridium butyricum were inserted into the fermentation medium for fermentation experiments. The inoculation ratios were 2%, 4%, 6%, 8%, and 10%, the number of colonies of the bacterial agent was measured, and the results are shown in Table 1.

[0026] Table 1 The impact of different bacterial species ratios on the number of live bacteria in Clostridium butyricum inoculum

[0027]

Embodiment 2

[0029] The present invention investigates a kind of Clostridium butyricum and its application for feeding additives, inserts the compound micro-ecological probiotic agent into the fermentation medium with the inoculation amount of 6% (V / V), and the fermentation temperature is 25 °C, 30 °C, 32 °C, 35 °C, 37 °C, 40 °C, measure the total number of colonies, cfu / g, total acid (calculated as lactic acid), %, the results are shown in Table 2. Therefore, the optimum fermentation temperature is 35°C.

[0030] Table 2 Effects of different fermentation temperatures on the physical and chemical indicators of fermentation products

[0031]

[0032]

Embodiment 3

[0034] The present invention investigates a Clostridium butyricum used as a feed additive and its application, and compares the following three culture media: (1) Culture medium A: soluble starch 5~10g / L, corn flour 20~ 30g / L, passion fruit peel 10~20g / L, manganese sulfate 0.01~0.1g / L, magnesium sulfate 0.1~0.5g / L, yeast powder 10~20g / L, dipotassium hydrogen phosphate 0.1~1g / L, FeSO41~ 2g / L, rice flour wastewater instead of water as liquid supplement; (2) Medium B: soluble starch 5-10g / L, corn flour 20-30g / L, manganese sulfate 0.01-0.1g / L, magnesium sulfate 0.1-0.5g / L, yeast powder 10-20g / L, dipotassium hydrogen phosphate 0.1-1g / L, rice flour wastewater instead of water as liquid supplement; (3) medium C: soluble starch 5-10g / L, corn flour 20-30g / L L, manganese sulfate 0.01~0.1g / L, magnesium sulfate 0.1~0.5g / L, yeast powder 10~20g / L, dipotassium hydrogen phosphate 0.1~1g / L, FeSO41~2g / L, water as liquid supplement; determination The total number of colonies of the fermentatio...

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Abstract

The invention relates to the technical field of microbial feed additives and preparation, and discloses clostridium butyricum for feed additives and application of the clostridium butyricum, the preservation number of the clostridium butyricum is CGMCC No.22880, and the clostridium butyricum is preserved in Beijing Microbiological Culture Collection Center. According to the clostridium butyricum for the feed additive and the application of the clostridium butyricum, a fermentation strain is innovated, the clostridium butyricum is selected as a feed probiotic agent, and the clostridium butyricum adapts to various conditions and has the effects of adjusting animal intestinal microorganism balance, improving animal feed conversion rate, effectively improving organism immunity and the like; the passion fruit peel and the rice flour wastewater are used for fermentation culture, so that the production cost is greatly reduced, waste is turned into wealth, the comprehensive benefits of production are improved, and the problems that the utilization rate of the rice flour wastewater in the production process is extremely low, most of the rice flour wastewater and the passion fruit peel are directly discharged and thrown away as wastes, and environmental pollution and resource waste are caused are solved.

Description

technical field [0001] The invention relates to the technical field of microbial feed additives and preparation thereof, in particular to a Clostridium butyricum used as a feed additive and an application thereof. Background technique [0002] Probiotics are a kind of live bacterial preparations that can improve the growth performance of animals by regulating the microbial balance in the intestinal tract of animals, promoting the growth and reproduction of normal flora in the gastrointestinal tract of animals, and inhibiting the growth and reproduction of pathogenic bacteria. At the same time, probiotics can replace antibiotics As a veterinary drug and feed additive, it has been widely valued and applied. Clostridium butyricum can improve the balance of the host's intestinal bacterial ecosystem, and effectively decompose the anti-nutritional factors in the bean dregs, so that the nutrients in the fermented passion fruit peel and rice flour wastewater are more balanced. [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/145
CPCC12N1/20A23K10/18
Inventor 韦朝英苏智兴李晓明周志强李从罗石林陆迪
Owner 南宁市拜欧生物工程有限责任公司
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